Identification of Glyoxalase-I as a Protein Marker in a Mouse Model of Extremes in Trait Anxiety

For >15 generations, CD1 mice have been selectively and bidirectionally bred for either high-anxiety-related behavior (HAB-M) or low-anxiety-related behavior (LAB-M) on the elevated plus-maze. Independent of gender, HAB-M were more anxious than LAB-M animals in a variety of additional tests, incl...

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Bibliographic Details
Published in:	The Journal of neuroscience Vol. 25; no. 17; pp. 4375 - 4384
Main Authors:	Kromer, Simone A, Kessler, Melanie S, Milfay, Dale, Birg, Isabel N, Bunck, Mirjam, Czibere, Ludwig, Panhuysen, Markus, Putz, Benno, Deussing, Jan M, Holsboer, Florian, Landgraf, Rainer, Turck, Christoph W
Format:	Journal Article
Language:	English
Published:	United States Soc Neuroscience 27-04-2005 Society for Neuroscience
Subjects:	Analysis of Variance Animals Animals, Newborn Anti-Anxiety Agents - administration & dosage Anxiety Disorders - diagnosis Anxiety Disorders - drug therapy Anxiety Disorders - enzymology Anxiety Disorders - genetics Avoidance Learning - drug effects Avoidance Learning - physiology Behavior, Animal Behavioral/Systems/Cognitive Biomarkers - metabolism Blotting, Western - methods Brain - drug effects Brain - metabolism Breeding - methods Diazepam - administration & dosage Disease Models, Animal Electrophoresis, Gel, Two-Dimensional - methods Exploratory Behavior - drug effects Exploratory Behavior - physiology Female Hindlimb Suspension - physiology Lactoylglutathione Lyase - isolation & purification Lactoylglutathione Lyase - metabolism Locomotion - genetics Male Mass Spectrometry - methods Mice Microarray Analysis - methods Phenotype Predictive Value of Tests Proteomics - methods Reaction Time - physiology Reproducibility of Results Sex Factors Spatial Behavior - drug effects Spatial Behavior - physiology Statistics as Topic Swimming Time Factors Vocalization, Animal - drug effects Vocalization, Animal - physiology
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Summary:	For >15 generations, CD1 mice have been selectively and bidirectionally bred for either high-anxiety-related behavior (HAB-M) or low-anxiety-related behavior (LAB-M) on the elevated plus-maze. Independent of gender, HAB-M were more anxious than LAB-M animals in a variety of additional tests, including those reflecting risk assessment behaviors and ultrasound vocalization, with unselected CD1 "normal" control (NAB-M) and cross-mated (CM-M) mice displaying intermediate behavioral scores in most cases. Furthermore, in both the forced-swim and tail-suspension tests, LAB-M animals showed lower scores of immobility than did HAB-M and NAB-M animals, indicative of a reduced depression-like behavior. Using proteomic and microarray analyses, glyoxalase-I was identified as a protein marker, which is consistently expressed to a higher extent in LAB-M than in HAB-M mice in several brain areas. The same phenotype-dependent difference was found in red blood cells with NAB-M and CM-M animals showing intermediate expression profiles of glyoxalase-I. Additional studies will examine whether glyoxalase-I has an impact beyond that of a biomarker to predict the genetic predisposition to anxiety- and depression-like behavior.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0270-6474 1529-2401
DOI:	10.1523/JNEUROSCI.0115-05.2005