Photodynamic therapy for American cutaneous leishmaniasis: The efficacy of methylene blue in hamsters experimentally infected with Leishmania (Leishmania) amazonensis

[Display omitted] . ► PDT promoted a decrease in the thickness of infected footpad in experimental ACL. ► PDT promoted reduction in the parasitic load in experimental ACL. ► MB+LED in ACL by Leishmania amazonensis shows a strong photodynamic effect. ► PDT using MB+LED is very promising in ACL caused...

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Published in:	Experimental parasitology Vol. 128; no. 4; pp. 353 - 356
Main Authors:	Peloi, Lílian Somenci, Biondo, Carlos Eduardo Guerino, Kimura, Elza, Politi, Mario José, Lonardoni, Maria Valdrinez Campana, Aristides, Sandra Mara Alessi, Dorea, Regina Coeli Cunha, Hioka, Noboru, Silveira, Thaís Gomes Verzignassi
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier Inc 01-08-2011 Elsevier
Subjects:	Animals Biological and medical sciences Cricetinae Cutaneous leishmaniasis Enzyme Inhibitors - standards Enzyme Inhibitors - therapeutic use Fundamental and applied biological sciences. Psychology hamsters Leishmania amazonensis Leishmania mexicana - drug effects Leishmaniasis, Cutaneous - drug therapy Life cycle. Host-agent relationship. Pathogenesis Light-Emitting-Diode lymph nodes Lymph Nodes - parasitology Male Mesocricetus Methylene Blue Methylene Blue - standards Methylene Blue - therapeutic use parasite load parasitology patients Photochemotherapy - methods Photochemotherapy - standards Photodynamic Therapy Protozoa Spleen - parasitology therapeutics Cutaneous leishmaniasis Methylene Blue Photodynamic Therapy Light-Emitting-Diode Leishmania amazonensis Kinetoplastida Protozoa Rodentia Parasite Light emitting diode Vertebrata Mammalia Hamster
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Summary:	[Display omitted] . ► PDT promoted a decrease in the thickness of infected footpad in experimental ACL. ► PDT promoted reduction in the parasitic load in experimental ACL. ► MB+LED in ACL by Leishmania amazonensis shows a strong photodynamic effect. ► PDT using MB+LED is very promising in ACL caused by Leishmania amazonensis. The aim of this study was to investigate the effectiveness of Photodynamic Therapy (PDT) using Methylene Blue (MB) as the photosensitizing compound and a Light-Emitting Diode (LED) in American cutaneous leishmaniasis (ACL). Hamsters were experimentally infected with Leishmania (Leishmania) amazonensis. After the development of the lesions in the footpad, the animals were treated with MB three times a week for 3months. Ten minutes after each application of MB, the lesions were irradiated with LED for 1h. The lesions were evaluated weekly by the measurement of the hamster footpad thickness. At the end of the treatment the parasitic load was quantified in the regional lymph node of the hamsters. The treatment promoted a decrease in the thickness of infected footpad (P=0.0001) and reduction in the parasitic load in the regional lymph node (P=0.0007) of the animals from group treated with MB+LED. PDT using MB+LED in ACL caused by L. amazonensis shows a strong photodynamic effect. This therapy is very promising, once it is an inexpensive system and the own patient can apply it in their wound and in their house without the need of technical assistance.
Bibliography:	http://dx.doi.org/10.1016/j.exppara.2011.04.009 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0014-4894 1090-2449
DOI:	10.1016/j.exppara.2011.04.009