Search Results - "BioTechniques"

PCR past, present and future by Zhu, Hanliang, Zhang, Haoqing, Xu, Ying, Laššáková, Soňa, Korabečná, Marie, Neužil, Pavel

“…PCR has become one of the most valuable techniques currently used in bioscience, diagnostics and forensic science. Here we review the history of PCR…”
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Recent advances in photo-crosslinkable hydrogels for biomedical applications by Choi, Jane Ru, Yong, Kar Wey, Choi, Jean Yu, Cowie, Alistair C

“…Photo-crosslinkable hydrogels have recently attracted significant scientific interest. Their properties can be manipulated in a spatiotemporal manner through…”
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Enhancing colorimetric loop-mediated isothermal amplification speed and sensitivity with guanidine chloride by Zhang, Yinhua, Ren, Guoping, Buss, Jackson, Barry, Andrew J, Patton, Gregory C, Tanner, Nathan A

“…Loop-mediated isothermal amplification (LAMP) is a versatile technique for detection of target DNA and RNA, enabling rapid molecular diagnostic assays with…”
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Library construction for next-generation sequencing: overviews and challenges by Head, Steven R, Komori, H Kiyomi, LaMere, Sarah A, Whisenant, Thomas, Van Nieuwerburgh, Filip, Salomon, Daniel R, Ordoukhanian, Phillip

“…High-throughput sequencing, also known as next-generation sequencing (NGS), has revolutionized genomic research. In recent years, NGS technology has steadily…”
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Molecularly imprinted polymers in biological applications by El-Schich, Zahra, Zhang, Yuecheng, Feith, Marek, Beyer, Sarah, Sternbæk, Louise, Ohlsson, Lars, Stollenwerk, Maria, Wingren, Anette Gjörloff

“…Molecularly imprinted polymers (MIPs) are currently widely used and further developed for biological applications. The MIP synthesis procedure is a key…”
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Western blotting (immunoblotting): history, theory, uses, protocol and problems by Sule, Rasheed, Rivera, Gabriela, Gomes, Aldrin V

“…Western blotting (immunoblotting) is a powerful and commonly used technique that is capable of detecting or semiquantifying an individual protein from complex…”
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Overlooked benefits of using polyclonal antibodies by Ascoli, Carl A, Aggeler, Birte

“…The benefits of polyclonal antibodies as tools for assay-specific target discovery and detection are numerous. As the future of basic research, diagnostics and…”
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Adenovirus vector system: construction, history and therapeutic applications by Syyam, Anum, Nawaz, Amjad, Ijaz, Aroosa, Sajjad, Umar, Fazil, Anila, Irfan, Sofia, Muzaffar, Aleeha, Shahid, Muhammad, Idrees, Muhammad, Malik, Kausar, Afzal, Samia

“…Since the isolation of adenovirus (AdV) in 1953, AdVs have been used as vectors for various therapeutic purposes, such as gene therapy in cancers and other…”
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Flow cytometry: past and future by Robinson, J Paul

“…Flow cytometry is a single-cell technology that measures scatter and fluorescence to establish a set of unique cellular properties. Flow cytometry is used in…”
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Optimization of staining with SYTO 9/propidium iodide: interplay, kinetics and impact on Brevibacillus brevis by Deng, Ying, Wang, Lili, Chen, Yujia, Long, Yan

“…Fluorophores SYTO 9 and propidium iodide (PI) are extensively applied in medicine, food industry and environmental monitoring to assess the viability of…”
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Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue by Goto, Motoki, Honda, Eiichi, Ogura, Atsuo, Nomoto, Akio, Hanaki, Ken-Ichi

“…Loop-mediated isothermal amplification (LAMP), a novel gene amplification method, enables the synthesis of larger amounts of both DNA and a visible…”
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Visiting "noncodarnia" by Perkel, Jeffrey M

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Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids by Bryksin, Anton V, Matsumura, Ichiro

“…Here we describe a straightforward, efficient, and reliable way to clone an insert of choice into a plasmid of choice without restriction endonucleases or T4…”
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ImageJ for microscopy by Collins, Tony J

“…ImageJ is an essential tool for us that fulfills most of our routine image processing and analysis requirements. The near-comprehensive range of import filters…”
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Twenty-five years of quantitative PCR for gene expression analysis by VanGuilder, Heather D, Vrana, Kent E, Freeman, Willard M

“…Following its invention 25 years ago, PCR has been adapted for numerous molecular biology applications. Gene expression analysis by reverse-transcription…”
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Western blotting: a powerful staple in scientific and biomedical research by Begum, Habeebunnisa, Murugesan, Periyasamy, Tangutur, Anjana Devi

“…Western blotting (WB), also known as immunoblotting, is a well-known molecular biology method that biologists often use to investigate many features of the…”
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Development of multiplexed reverse-transcription loop-mediated isothermal amplification for detection of SARS-CoV-2 and influenza viral RNA by Zhang, Yinhua, Tanner, Nathan A

“…The ongoing pandemic has demonstrated the utility of widespread surveillance and diagnostic detection of the novel SARS-CoV-2. Reverse-transcription…”
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TScratch: a novel and simple software tool for automated analysis of monolayer wound healing assays by Gebäck, Tobias, Schulz, Martin Michael Peter, Koumoutsakos, Petros, Detmar, Michael

“…Cell migration plays a major role in development, physiology, and disease, and is frequently evaluated in vitro by the monolayer wound healing assay. The assay…”
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Rapid quantification of cellular proliferation and migration using ImageJ by Venter, Niesler, CU

“…Cellular proliferation and migration are crucial during development, regeneration and disease. Methods to quantify these processes are available; however, many…”
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Comparison of fluorescent intercalating dyes for quantitative loop-mediated isothermal amplification (qLAMP) by Oscorbin, Igor P, Belousova, Ekaterina A, Zakabunin, Aleksandr I, Boyarskikh, Ulyana A, Filipenko, Maksim L

“…Real-time or quantitative loop-mediated isothermal amplification (qLAMP) is a promising technique for the accurate detection of pathogens in organisms and the…”
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