Search Results - "Bingle, Wade H"

Caulobacter crescentus Synthesizes an S-Layer-Editing Metalloprotease Possessing a Domain Sharing Sequence Similarity with Its Paracrystalline S-Layer Protein by Umelo-Njaka, Elizabeth, Bingle, Wade H, Borchani, Faten, Le, Khai D, Awram, Peter, Blake, Theo, Nomellini, John F, Smit, John

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V. Functions of S-layers by Beveridge, Terrance J, Pouwels, Peter H, Sára, Margit, Kotiranta, Anja, Lounatmaa, Kari, Kari, Kirsti, Kerosuo, Eero, Haapasalo, Markus, Egelseer, Eva M, Schocher, Ingrid, Sleytr, Uwe B, Morelli, Lorenzo, Callegari, Maria-Luisa, Nomellini, John F, Bingle, Wade H, Smit, John, Leibovitz, Emmanuelle, Lemaire, Marc, Miras, Isabelle, Salamitou, Sylvie, Béguin, Pierre, Ohayon, Hélène, Gounon, Pierre, Matuschek, Markus, Sahm, Kerstin, Bahl, Hubert, Grogono-Thomas, Rosemary, Dworkin, Joel, Blaser, Martin J, Woodland, Ralph M, Newell, Diane G, Kessel, Martin, Koval, Susan F

“…Although S-layers are being increasingly identified on Bacteria and Archaea, it is enigmatic that in most cases S-layer function continues to elude us. In a…”
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Evaluation of a new system for developing particulate enzymes based on the surface (S)-layer protein (RsaA) of Caulobacter crescentus: Fusion with the β-1,4-glycanase (Cex) from the cellulolytic bacterium Cellulomonas fimi yields a robust, catalytically active product by Duncan, G, Tarling, C.A, Bingle, W.H, Nomellini, J.F, Yamage, M, Dorocicz, I.R, Withers, S.G, Smit, J

“…Immobilized biocatalysts, including particulate enzymes, represent an attractive tool for research and industrial applications because they combine the…”
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Expression and testing of Pseudomonas aeruginosa vaccine candidate proteins prepared with the Caulobacter crescentus S-layer protein expression system by Umelo-Njaka, Elizabeth, Nomellini, John F., Bingle, Wade H., Glasier, Linda G.M., Irvin, Randall T., Smit, John

“…A novel bacterial protein secretion system was used to produce vaccine candidates against Pseudomonas aeruginosa. The surface protein (RsaA) of Caulobacter…”
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Evaluation of a New System for Developing Particulate Enzymes Based on the Surface (S)-Layer Protein (RsaA) of Caulobacter crescentus: Fusion With the b-1,4-Glycanase (Cex) From the Cellulolytic Bacterium Cellulomonas fimi Yields a Robust, Catalytically Active Product by Duncan, Gillian, Tarling, Chris A., Bingle, Wade H., Nomellini, John F., Yamage, Mat, Dorocicz, Irene R., Withers, Stephen G., Smit, John

“…Immobilized biocatalysts, including particulate enzymes, represent an attractive tool for research and industrial applications because they combine the…”
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The extreme N-terminus of the Caulobacter crescentus surface-layer protein directs export of passenger proteins from the cytoplasm but is not required for secretion of the native protein by Bingle, W H, Le, K D, Smit, J

“…The paracrystalline surface layer (S-layer) of Caulobacter crescentus is composed of a single protein (RsaA, 1026 amino acids) that associates noncovalently…”
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Scientific Literacy for Decisionmaking and the Social Construction of Scientific Knowledge by Bingle, Wade H, Gaskell, P. James

“…Explores two ways of critically examining scientific knowledge in the context of a current socioscientific dispute: the unsterilized spacecraft in NASA's…”
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Alkaline phosphatase and a cellulase reporter protein are not exported from the cytoplasm when fused to large N-terminal portions of the Caulobacter crescentus surface (S)-layer protein by Bingle, W H, Smit, J

“…Using a gene fusion approach, hybrid proteins were created by linking alkaline phosphatase (PhoA) or a cellulase reporter (delta CenA) to four large N-terminal…”
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V. Functions of S-layers by Beveridge, Terrance J., Pouwels, Peter H., Sára, Margit, Kotiranta, Anja, Lounatmaa, Kari, Kari, Kirsti, Kerosuo, Eero, Haapasalo, Markus, Egelseer, Eva M., Schocher, Ingrid, Sleytr, Uwe B., Morelli, Lorenzo, Callegari, Maria-Luisa, Nomellini, John F., Bingle, Wade H., Smit, John, Leibovitz, Emmanuelle, Lemaire, Marc, Miras, Isabelle, Salamitou, Sylvie, Béguin, Pierre, Ohayon, Hélène, Gounon, Pierre, Matuschek, Markus, Sahm, Kerstin, Bahl, Hubert, Grogono-Thomas, Rosemary, Dworkin, Joel, Blaser, Martin J., Woodland, Ralph M., Newell, Diane G., Kessel, Martin, Koval, Susan F.

“…Abstract Although S-layers are being increasingly identified on Bacteria and Archaea, it is enigmatic that in most cases S-layer function continues to elude…”
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An "all-purpose" cellulase reporter for gene fusion studies and application to the paracrystalline surface (S)-layer protein of Caulobacter crescentus by Bingle, W H, Kurtz, Jr, H D, Smit, J

“…The secreted endoglucanase (CenA) from the Gram-positive bacterium Cellulomonas fimi and a deletion derivative (delta CenA) lacking the N-terminal leader…”
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Secretion of the Caulobacter crescentus S-layer protein: further localization of the C-terminal secretion signal and its use for secretion of recombinant proteins by Bingle, W H, Nomellini, J F, Smit, J

“…The secretion signal of the Caulobacter crescentus S-layer protein (RsaA) was localized to the C-terminal 82 amino acids of the molecule. Protein yield studies…”
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Cell‐surface display of a Pseudomonas aeruginosa strain K pilin peptide within the paracrystalline S‐layer of Caulobacter crescentus by Bingle, Wade H., Nomellini, John F., Smit, John

“…The paracrystalline surface (S)‐layer of Caulobacter crescentus is composed of a single secreted protein (RsaA) that interlocks in a hexagonal pattern to…”
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Evaluation of a new system for developing particulate enzymes based on the surface (S)-layer protein (RsaA) of Caulobacter crescentus: Fusion with the [beta]-1,4-glycanase (cex) from the cellulolytic bacterium cellulomonas fimi yields a robust, catalytically active product by Duncan, Gilian, Tarling, Chris A, Bingle, Wade H, Nomellini, John F, Yamage, Mat, Dorocicz, Irene R, Withers, Stephen G, Smit, John

“…Immobilized biocatalysts, including particulate enzymes, represent an attractive tool for research and industrial applications because they combine the…”
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Secretion of the Caulobacter crescentusS-Layer Protein: Further Localization of the C-Terminal Secretion Signal and Its Use for Secretion of Recombinant Proteins by Wade H. Bingle, John F. Nomellini, John Smit

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Secretion of the Caulobacter crescentus S-layer protein: Further localization of the C-terminal secretion signal and its use for secretion of recombinant patterns by Bingle, Wade H, Nomellini, John F, Smit, John

“…The secretion signal of the Caulobacter crescentus S-layer protein (RsaA) was localized to the C-terminal 82 amino acids of the molecule. Protein yield studies…”
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Linker mutagenesis of the Caulobacter crescentus S-layer protein: toward a definition of an N-terminal anchoring region and a C-terminal secretion signal and the potential for heterologous protein secretion by Bingle, W H, Nomellini, J F, Smit, J

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Linker mutagensis of the Caulobacter crescentus S-layer protein: Toward a definition of an N-terminal anchoring region and a C-terminal secretion signal and the potential for heterologous protein secretion by Bingle, Wade H, Nomellini, John F, Smit, John

“…Bingle et al use linker insertion mutagenesis of the Caulobacter crescentus S-layer protein gene to more precisely identify functional regions of the protein…”
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V. Functions of S‐layers1 by Beveridge, Terrance J, Pouwels, Peter H, Sára, Margit, Kotiranta, Anja, Lounatmaa, Kari, Kari, Kirsti, Kerosuo, Eero, Haapasalo, Markus, Egelseer, Eva M, Schocher, Ingrid, Sleytr, Uwe B, Morelli, Lorenzo, Callegari, Maria‐Luisa, Nomellini, John F, Bingle, Wade H, Smit, John, Leibovitz, Emmanuelle, Lemaire, Marc, Miras, Isabelle, Salamitou, Sylvie, Béguin, Pierre, Ohayon, Hélène, Gounon, Pierre, Matuschek, Markus, Sahm, Kerstin, Bahl, Hubert, Grogono‐Thomas, Rosemary, Dworkin, Joel, Blaser, Martin J, Woodland, Ralph M, Newell, Diane G, Kessel, Martin, Koval, Susan F

“…Although S‐layers are being increasingly identified on Bacteria and Archaea, it is enigmatic that in most cases S‐layer function continues to elude us. In a…”
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Transformation of Azotobacter vinelandii OP with a broad host range plasmid containing a cloned chromosomal nif-DNA marker by Bingle, W H

“…The non-nitrogen-fixing (Nif-) strain UW10 of Azotobacter vinelandii OP (UW) was naturally induced to competence and transformed with broad host range plasmid…”
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High-level expression vectors for Caulobacter crescentus incorporating the transcription/translation initiation regions of the paracrystalline surface-layer-protein gene by Bingle, W H, Smit, J

“…A number of plasmid vectors were constructed for high-level gene expression in the dimorphic gram-negative bacterium Caulobacter crescentus. These vectors…”
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