Production and purification of alkaline xylanases

This work investigated more than 500 colonies with xylanolytic activity which were able to grow in a medium containing corn xylan as the only carbon source. Out of the 500 colonies, 22 microorganisms were also able to grow in birchwood xylan and were cellulase-free producers. The xylanase activity w...

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Bibliographic Details
Published in:	Bioresource technology Vol. 68; no. 1; pp. 49 - 53
Main Authors:	Duarte, M.C.T., Portugal, E.P., Ponezi, A.N., Bim, M.A., Tagliari, C.V., Franco, T.T.
Format:	Journal Article Conference Proceeding
Language:	English
Published:	Oxford Elsevier Ltd 01-04-1999 Elsevier Science
Subjects:	Aalkaline xylanases Aqueous two-phase systems Bacillus pumilus Bacteria Biological and medical sciences Biotechnology Catalyst activity Downstream processing Enzyme engineering Enzyme purification Fermentation Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Phosphates Polyethylene glycols Polysaccharides Production of selected enzymes Protein partitioning Purification xylan Bacillus pumilus Aalkaline xylanases Aqueous two-phase systems Protein partitioning Enzyme purification Downstream processing Culture medium Purification Enzyme Xylan Vegetal by product Lignocellulosics Bacillaceae Fermentation Sodium chloride Xylanolytic enzyme Ethylene oxide polymer Screening Bacillales Enzymatic activity Microorganism culture Bacteria Corn by product Two phase medium Polysaccharide
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Summary:	This work investigated more than 500 colonies with xylanolytic activity which were able to grow in a medium containing corn xylan as the only carbon source. Out of the 500 colonies, 22 microorganisms were also able to grow in birchwood xylan and were cellulase-free producers. The xylanase activity was studied at pH 10.0 and pH 5.0. It was observed that the three best producers of alkaline xylanase yielded enzyme levels in the range of 2.6 to 4.0 U/ml. Enzyme levels of 1.0 to 1.25 U/ml were achieved by four other microorganisms. Conversely, there were three microorganisms that produced a xylanase which was mostly active at pH 5.0. There was just one microorganism able to produce an enzyme active at pH 10.0. The alkaline xylanases from crude fermentation broth were extracted in aqueous two-phase systems (ATPS) composed of 16% polyethyleneglycol (PEG 6000) and 8% phosphate salt. A purification factor of 57 and a 41% yield of enzyme activity were achieved for the system containing 16% PEG 6000, 8% K 2HPO 4 and 12% NaCl.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0960-8524 1873-2976
DOI:	10.1016/S0960-8524(98)00079-0