Verifying reference intervals for coagulation tests by using stored data

Verifying reference intervals for coagulation tests by using stored data

Abstract Objective: The purpose of the study was to verify the reference intervals for prothrombin time (PT) and activated partial thromboplastin time (APTT), using stored data of ambulatory pre-op subjects with exclusion of certain clinics, according to age and sex. Materials and Methods: Results o...

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Bibliographic Details
Published in:Scandinavian journal of clinical and laboratory investigation Vol. 71; no. 8; pp. 647 - 652
Main Authors: Aral, Hale, Usta, Murat, Cilingirturk, Ahmet M., Inal, Berrin B., Bilgi, Pinar T., Guvenen, Guvenc
Format: Journal Article
Language:English
Published: Colchester Informa Healthcare 01-12-2011
Taylor & Francis
Informa
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Algorithms
Biological and medical sciences
Blood Coagulation
Blood coagulation tests
Clinical Laboratory Techniques - standards
Clinical Laboratory Techniques - statistics & numerical data
data storage
Databases, Factual
Female
hematology
Humans
Investigative techniques, diagnostic techniques (general aspects)
Male
Medical sciences
Middle Aged
Outpatients
Partial Thromboplastin Time - standards
Partial Thromboplastin Time - statistics & numerical data
Prothrombin Time - standards
Prothrombin Time - statistics & numerical data
reference ranges
Reference Values
Sex Factors
statistical data analysis
Blood coagulation
Reference value
Data analysis
Statistical analysis
Hematology
Conservation
reference ranges
Standards
data storage
Storage
Coagulation test
Clinical biology
Blood coagulation tests
statistical data analysis
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Summary:Abstract Objective: The purpose of the study was to verify the reference intervals for prothrombin time (PT) and activated partial thromboplastin time (APTT), using stored data of ambulatory pre-op subjects with exclusion of certain clinics, according to age and sex. Materials and Methods: Results of test requests (13,600 PT and 14,083 APTT) of subjects aged 15-80 made from outpatient clinics of surgical departments before surgical interventions in 2008 were retrieved from the electronic medical record. Thromborel S and Actin (Dade Behring, Germany) were used on the Sysmex® CA-1500 coagulation analyzer. Extreme values were determined by using Horn's algorithm after Box-Cox transformation, and the upper and lower reference limits were determined as the 2.5th and 97.5th percentiles of the cleaned data. Results: The values outside the interval of PT data 10.5-17.0 seconds and the interval of APTT data 20.6-35.8 seconds were excluded from the analysis. There were significant differences among age subsets of PT measurements ( p < 0.0001) and of APTT measurements ( p < 0.0001). Accordingly, the data were tested for gender differences and a significant difference was found in PT ( p = 0.002). APPT results did not differ statistically between men and women. Conclusion: Although we found values different from the limits stated in the kit insert, it would be better to confirm our findings with the direct method, especially in APTT for patients under the age of 40 and over the age of 59, and also for sex differences in PT.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0036-5513
1502-7686
DOI:10.3109/00365513.2011.618546