Search Results - "Bienert, Michael"

Solid-phase peptide synthesis: from standard procedures to the synthesis of difficult sequences by Coin, Irene, Beyermann, Michael, Bienert, Michael

“…This protocol for solid-phase peptide synthesis (SPPS) is based on the widely used Fmoc/tBu strategy, activation of the carboxyl groups by aminium-derived…”
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Synthesis of Biologically Active Peptide Nucleic Acid−Peptide Conjugates by Sortase-Mediated Ligation by Pritz, Stephan, Wolf, Yvonne, Kraetke, Oliver, Klose, Jana, Bienert, Michael, Beyermann, Michael

“…Sortase A is a transpeptidase that cleaves at a pentapeptide-motif and subsequently transfers the acyl component to a nucleophile containing N-terminal…”
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Optimization of the antimicrobial activity of magainin peptides by modification of charge by Dathe, Margitta, Nikolenko, Heike, Meyer, Jana, Beyermann, Michael, Bienert, Michael

“…Investigation of magainin II amide analogs with cationic charges ranging between +3 and +7 showed that enhancement of the peptide charge up to a threshold…”
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Depsipeptide Methodology for Solid-Phase Peptide Synthesis:  Circumventing Side Reactions and Development of an Automated Technique via Depsidipeptide Units by Coin, Irene, Dölling, Rudolf, Krause, Eberhard, Bienert, Michael, Beyermann, Michael, Sferdean, Calin Dan, Carpino, Louis A

“…The depsipeptide technique is a recently developed method for peptide synthesis which is applicable to difficult sequences when the synthetic difficulty arises…”
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Solid-Phase Synthesis of a Cyclodepsipeptide: Cotransin by Coin, Irene, Beerbaum, Monika, Schmieder, Peter, Bienert, Michael, Beyermann, Michael

“…The first solid-phase synthesis of cotransina cyclic depsipeptide having high pharmacological potentialwas achieved, by a proper choice of coupling reagents…”
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General aspects of peptide selectivity towards lipid bilayers and cell membranes studied by variation of the structural parameters of amphipathic helical model peptides by Dathe, Margitta, Meyer, Jana, Beyermann, Michael, Maul, Björn, Hoischen, Christian, Bienert, Michael

“…Model compounds of modified hydrophobicity ( H), hydrophobic moment ( μ) and angle subtended by charged residues ( Φ) were synthesized to define the general…”
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A Critical Reassessment of Penetratin Translocation Across Lipid Membranes by Bárány-Wallje, Elsa, Keller, Sandro, Serowy, Steffen, Geibel, Sebastian, Pohl, Peter, Bienert, Michael, Dathe, Margitta

“…Penetratin is a short, basic cell-penetrating peptide able to induce cellular uptake of a vast variety of large, hydrophilic cargos. We have reassessed the…”
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Phosphopeptide analysis by positive and negative ion matrix-assisted laser desorption/ionization mass spectrometry by Janek, Katharina, Wenschuh, Holger, Bienert, Michael, Krause, Eberhard

“…This article describes a simple procedure for the detection of phosphorylated peptides by comparable positive and negative ion mode matrix‐assisted laser…”
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Hydrophobicity, hydrophobic moment and angle subtended by charged residues modulate antibacterial and haemolytic activity of amphipathic helical peptides by Dathe, Margitta, Wieprecht, Torsten, Nikolenko, Heike, Handel, Liselotte, Maloy, W.Lee, MacDonald, Dorothy L, Beyermann, Michael, Bienert, Michael

“…The hydrophobicity (H), hydrophobic moment (μ) and the angle subtended by the positively charged helix face (Φ) of a set of model and magainin 2 amide peptides…”
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Evidence for extensive non-endocytotic translocation of peptide nucleic acids across mammalian plasma membranes by Oehlke, Johannes, Turner, Yvonne, Pritz, Stephan, Bienert, Michael

“…The ability of peptide nucleic acids (PNA) to enter and to cross filter-grown MDCK, HEK and CHO cells was studied by means of a protocol based on capillary…”
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The depsipeptide technique applied to peptide segment condensation: Scope and limitations by Coin, Irene, Schmieder, Peter, Bienert, Michael, Beyermann, Michael

“…A promising application of the depsipeptide technique has recently been proposed to provide ideal conditions for segment condensation, in that coupling of…”
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Regulation of the Coupling to Different G Proteins of Rat Corticotropin-releasing Factor Receptor Type 1 in Human Embryonic Kidney 293 Cells by Wietfeld, Doreen, Heinrich, Nadja, Furkert, Jens, Fechner, Klaus, Beyermann, Michael, Bienert, Michael, Berger, Hartmut

“…The regulation of G protein activation by the rat corticotropin-releasing factor receptor type 1 (rCRFR1) in human embryonic kidney (HEK)293 (HEK-rCRFR1) cell…”
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Late endosomal/lysosomal targeting and lack of recycling of the ligand-occupied endothelin B receptor by Oksche, A, Boese, G, Horstmeyer, A, Furkert, J, Beyermann, M, Bienert, M, Rosenthal, W

“…A fusion protein consisting of the endothelin B (ET(B)) receptor and the enhanced green fluorescent protein (EGFP) in conjunction with Cyanin3- or…”
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Influence of the Angle Subtended by the Positively Charged Helix Face on the Membrane Activity of Amphipathic, Antibacterial Peptides by Wieprecht, Torsten, Dathe, Margitta, Epand, Richard M, Beyermann, Michael, Krause, Eberhard, Maloy, W. Lee, MacDonald, Dorothy L, Bienert, Michael

“…To investigate the influence of the angle subtended by the positively charged helix face on membrane activity, six amphipathic α-helical peptides with angles…”
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Modulation of membrane activity of amphipathic, antibacterial peptides by slight modifications of the hydrophobic moment by Wieprecht, Torsten, Dathe, Margitta, Krause, Eberhard, Beyermann, Michael, Maloy, W.Lee, MacDonald, Dorothy L, Bienert, Michael

“…Starting from the sequences of magainin 2 analogs, peptides with slightly increased hydrophobic moment ( μ) but retained other structural parameters were…”
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Complex Polyfluoride Additives in Fmoc-Amino Acid Fluoride Coupling Processes. Enhanced Reactivity and Avoidance of Stereomutation by Carpino, Louis A, Ionescu, Dumitru, El-Faham, Ayman, Beyermann, Michael, Henklein, Peter, Hanay, Christiane, Wenschuh, Holger, Bienert, Michael

“…Isolated Fmoc amino acid fluorides have previously been shown to be among the most efficient reagents for peptide bond formation. Now, it has been found that…”
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A Role for a Helical Connector between Two Receptor Binding Sites of a Long-chain Peptide Hormone by Beyermann, Michael, Rothemund, Sven, Heinrich, Nadja, Fechner, Klaus, Furkert, Jens, Dathe, Margitta, Winter, Rüdiger, Krause, Eberhard, Bienert, Michael

“…The conformational freedom of single-chain peptide hormones, such as the 41-amino acid hormone corticotropin releasing factor (CRF), is a major obstacle to the…”
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Location of an Amphipathic .alpha.-Helix in Peptides Using Reversed-Phase HPLC Retention Behavior of D-Amino Acid Analogs by Krause, Eberhard, Beyermann, Michael, Dathe, Margitta, Rothemund, Sven, Bienert, Michael

“…The reversed-phase HPLC retention behavior of D-amino acid replacement sets of an amphipathic model peptide, neuropeptide Y, and corticotropin releasing factor…”
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Peptide Leucine Arginine, a Potent Immunomodulatory Peptide Isolated and Structurally Characterized from the Skin of the Northern Leopard Frog, Rana pipiens by Salmon, Amanda L., Cross, Laurence J.M., Irvine, Alexandra E., Lappin, Terry R.J., Dathe, Margitta, Krause, Gerd, Canning, Paul, Thim, Lars, Beyermann, Michael, Rothemund, Sven, Bienert, Michael, Shaw, Chris

“…On the basis of histamine release from rat peritoneal mast cells, an octadecapeptide was isolated from the skin extract of the Northern Leopard frog (Rana…”
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Comparison of the Effects of 5- and 6-HOAt on Model Peptide Coupling Reactions Relative to the Cases for the 4- and 7-Isomers by Carpino, Louis A, Imazumi, Hideko, Foxman, Bruce M, Vela, Michael J, Henklein, Peter, El-Faham, Ayman, Klose, Jana, Bienert, Michael

“…Synthesis of 5- and 6-HOAt has completed the full set of the four HOAt isomers derived from HOBt by insertion of a single nitrogen atom in the benzenoid…”
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