Biaxial cell stimulation: A mechanical validation

Abstract To analyse mechanotransduction resulting from tensile loading under defined conditions, various devices for in vitro cell stimulation have been developed. This work aimed to determine the strain distribution on the membrane of a commercially available device and its consistency with rising...

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Bibliographic Details
Published in:	Journal of biomechanics Vol. 42; no. 11; pp. 1692 - 1696
Main Authors:	Bieler, F.H, Ott, C.E, Thompson, M.S, Seidel, R, Ahrens, S, Epari, D.R, Wilkening, U, Schaser, K.D, Mundlos, S, Duda, G.N
Format:	Journal Article
Language:	English
Published:	United States Elsevier Ltd 07-08-2009 Elsevier Limited
Subjects:	Animals Biaxial tensile strain BioFlex Biomechanical Phenomena Bone Marrow Cells - cytology Cell Adhesion Cell Culture Techniques - methods Cell deformation Cells, Cultured Chickens Compressive Strength Digital image correlation Elasticity Equipment Design Fluorescent Dyes - pharmacology Membranes Microscopy, Fluorescence - methods Physical Medicine and Rehabilitation Programming languages Silicones - chemistry Software Stress, Mechanical Studies Tensile Strength BioFlex Digital image correlation Cell deformation Biaxial tensile strain
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Summary:	Abstract To analyse mechanotransduction resulting from tensile loading under defined conditions, various devices for in vitro cell stimulation have been developed. This work aimed to determine the strain distribution on the membrane of a commercially available device and its consistency with rising cycle numbers, as well as the amount of strain transferred to adherent cells. The strains and their behaviour within the stimulation device were determined using digital image correlation (DIC). The strain transferred to cells was measured on eGFP-transfected bone marrow-derived cells imaged with a fluorescence microscope. The analysis was performed by determining the coordinates of prominent positions on the cells, calculating vectors between the coordinates and their length changes with increasing applied tensile strain. The stimulation device was found to apply homogeneous (mean of standard deviations approx. 2% of mean strain) and reproducible strains in the central well area. However, on average, only half of the applied strain was transferred to the bone marrow-derived cells. Furthermore, the strain measured within the device increased significantly with an increasing number of cycles while the membrane's Young's modulus decreased, indicating permanent changes in the material during extended use. Thus, strain magnitudes do not match the system readout and results require careful interpretation, especially at high cycle numbers.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0021-9290 1873-2380
DOI:	10.1016/j.jbiomech.2009.04.013