Association between decreases in type V collagen and apoptosis in mouse lung chemical carcinogenesis: a preliminary model to study cancer cell behavior

The importance of type V collagen and its relationships with other types of collagen and with vascular and epithelial apoptosis were studied in a model of chemical carcinogenesis in the mouse lung. Two groups of male Balb/c mice were studied: a) animals that received two intraperitoneal doses of 3 g...

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Bibliographic Details
Published in:	Clinics (São Paulo, Brazil) Vol. 65; no. 4; pp. 425 - 432
Main Authors:	Parra, Edwin Roger, Bielecki, Leonardo Cavallari, Ribeiro, José Mauro da Fonseca Pestana, de Andrade Balsalobre, Fernando, Teodoro, Walcy R., Capelozzi, Vera Luiza
Format:	Journal Article
Language:	English
Published:	Brazil Elsevier España, S.L.U 01-04-2010 Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo Faculdade de Medicina / USP Elsevier España
Subjects:	Animals Apoptosis - physiology Biomarkers, Tumor - metabolism Carcinogens Caspase 9 - metabolism Clinical Sciences Collagen Type V - analysis Collagen Type V - metabolism Disease Models, Animal Experimental lung cancer Extracellular Matrix Immunofluorescence Imunohistochemistry Lung Neoplasms - chemically induced Lung Neoplasms - pathology Male MEDICINE, GENERAL & INTERNAL Mice Mice, Inbred BALB C Morphometry Type V collagen Urethane Imunohistochemistry Type V collagen Immunofluorescence Morphometry Experimental lung cancer
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Summary:	The importance of type V collagen and its relationships with other types of collagen and with vascular and epithelial apoptosis were studied in a model of chemical carcinogenesis in the mouse lung. Two groups of male Balb/c mice were studied: a) animals that received two intraperitoneal doses of 3 g/kg urethane carcinogen (urethane group = 24); and b) animals submitted to a sham procedure, comparable to the test group (control group = 7). Both groups were sacrificed after 120 days. In situ detection of apoptosis, immunohistochemistry, immunofluorescence and histomorphometry were used to evaluate the fraction occupied by the tumor, vascular and epithelial apoptosis, and type V, III and I collagen fibers in the lung parenchyma from both groups. The lung parenchyma from the urethane group showed low fractions of vascular and epithelial apoptosis as well as reduced type V collagen fibers when compared to the control group. A significant direct association was found between type V and III collagen fibers and epithelial apoptosis, type V collagen fibers and vascular apoptosis, and type V and type I collagen fibers. The results show that a direct link between low amounts of type V collagen and decreased cell apoptosis may favor cancer cell growth in the mouse lung after chemical carcinogenesis, suggesting that strategies aimed at preventing decreased type V collagen synthesis or local responses to reduced apoptosis may have a greater impact in lung cancer control.
ISSN:	1807-5932 1980-5322 1980-5322
DOI:	10.1590/S1807-59322010000400012