Pseudomonas aeruginosa responds to exogenous polyunsaturated fatty acids (PUFAs) by modifying phospholipid composition, membrane permeability, and phenotypes associated with virulence

Pseudomonas aeruginosa, a common opportunistic pathogen, is known to cause infections in a variety of compromised human tissues. An emerging mechanism for microbial survival is the incorporation of exogenous fatty acids to alter the cell's membrane phospholipid profile. With these findings, we...

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Bibliographic Details
Published in:	BMC microbiology Vol. 18; no. 1; p. 117
Main Authors:	Baker, Lyssa Y, Hobby, Chelsea R, Siv, Andrew W, Bible, William C, Glennon, Michael S, Anderson, Derek M, Symes, Steven J, Giles, David K
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 14-09-2018 BioMed Central BMC
Subjects:	Amides Antibiotics Antimicrobial peptides Bacteria Bacterial cell walls Biofilm Biofilms Chromatography Colistin Cystic fibrosis Drug resistance Ethidium bromide Fatty acids Gene expression Gram-positive bacteria Health aspects Human tissues Imipenem Lipids Liquid chromatography Mass spectrometry Mass spectroscopy Membrane permeability Metabolism Microorganisms Motility Nosocomial infections Opportunist infection Pathogens Peptide antibiotics Peptides Permeability Phenotypes Phosphatidylethanolamine Phosphatidylglycerol Phospholipid composition Phospholipids Physiological aspects Physiology Polymyxin B Polyunsaturated fatty acids Proteins Pseudomonas aeruginosa Studies Survival Swimming Thin layer chromatography Unsaturated fatty acids Virulence Virulence (Microbiology) Biofilm Pseudomonas aeruginosa Phospholipids Motility Fatty acids Antimicrobial peptides
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Summary:	Pseudomonas aeruginosa, a common opportunistic pathogen, is known to cause infections in a variety of compromised human tissues. An emerging mechanism for microbial survival is the incorporation of exogenous fatty acids to alter the cell's membrane phospholipid profile. With these findings, we show that exogenous fatty acid exposure leads to changes in bacterial membrane phospholipid structure, membrane permeability, virulence phenotypes and consequent stress responses that may influence survival and persistence of Pseudomonas aeruginosa. Thin-layer chromatography and ultra performance liquid chromatography / ESI-mass spectrometry indicated alteration of bacterial phospholipid profiles following growth in the presence of polyunsaturated fatty acids (PUFAs) (ranging in carbon length and unsaturation). The exogenously supplied fatty acids were incorporated into the major bacterial phospholipids phosphatidylethanolamine and phosphatidylglycerol. The incorporation of fatty acids increased membrane permeability as judged by both accumulation and exclusion of ethidium bromide. Individual fatty acids were identified as modifying resistance to the cyclic peptide antibiotics polymyxin B and colistin, but not the beta-lactam imipenem. Biofilm formation was increased by several PUFAs and significant fluctuations in swimming motility were observed. Our results emphasize the relevance and complexity of exogenous fatty acids in the membrane physiology and pathobiology of a medically important pathogen. P. aeruginosa exhibits versatility with regard to utilization of and response to exogenous fatty acids, perhaps revealing potential strategies for prevention and control of infection.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1471-2180 1471-2180
DOI:	10.1186/s12866-018-1259-8