A public genome-scale lentiviral expression library of human ORFs

Two sequence-verified, clonal, publicly available collections of human open reading frames are reported. One collection is in a lentiviral vector for expression in mammalian cells; the other is in the Gateway vector system. Functional characterization of the human genome requires tools for systemati...

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Bibliographic Details
Published in:	Nature methods Vol. 8; no. 8; pp. 659 - 661
Main Authors:	Yang, Xiaoping, Boehm, Jesse S, Yang, Xinping, Salehi-Ashtiani, Kourosh, Hao, Tong, Shen, Yun, Lubonja, Rakela, Thomas, Sapana R, Alkan, Ozan, Bhimdi, Tashfeen, Green, Thomas M, Johannessen, Cory M, Silver, Serena J, Nguyen, Cindy, Murray, Ryan R, Hieronymus, Haley, Balcha, Dawit, Fan, Changyu, Lin, Chenwei, Ghamsari, Lila, Vidal, Marc, Hahn, William C, Hill, David E, Root, David E
Format:	Journal Article
Language:	English
Published:	New York Nature Publishing Group US 01-08-2011 Nature Publishing Group
Subjects:	631/1647/666/2261 631/208/726/1912 631/326/596/2561 Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology brief-communication Cellular biology Cloning, Molecular - methods Gene expression Genetic aspects Genetic Vectors - genetics Genomic Library Genomics Humans Lentivirus - genetics Life Sciences Nucleotide sequence Open Reading Frames Physiological aspects Proteomics United States
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Summary:	Two sequence-verified, clonal, publicly available collections of human open reading frames are reported. One collection is in a lentiviral vector for expression in mammalian cells; the other is in the Gateway vector system. Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work.
ISSN:	1548-7091 1548-7105
DOI:	10.1038/nmeth.1638