Molecular characterization and expression analysis of Manduca sexta allatotropin

Juvenile hormones (JH) are a class of regulatory sesquiterpenoids that control metamorphosis in immature insects and reproduction in adult insects. The regulation of JH synthesis by the corpora allata (CA), a pair of endocrine glands with nervous connections to the brain, is achieved by a complex in...

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Bibliographic Details
Published in:	European journal of biochemistry Vol. 239; no. 3; pp. 588 - 596
Main Authors:	Taylor, P.A. III, Bhatt, T.R, Horodyski, F.M
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Science Ltd 01-08-1996
Subjects:	allatotropin Alternative Splicing Amino Acid Sequence animal breeding animal genetics animal physiology animal reproduction Animals arthropod pests arthropods Base Sequence DNA, Complementary - genetics entomology genbank/u62100 genbank/u62101 genbank/u62102 Gene Expression Genes, Insect Genomic Library in situ hybridization Insect Hormones - genetics Insect Hormones - isolation & purification juvenile hormone Manduca - genetics Manduca sexta Molecular Sequence Data Nerve Tissue - chemistry neuropeptides Neuropeptides - genetics Neuropeptides - isolation & purification plant health plant pests Polymerase Chain Reaction Protein Precursors - genetics RNA, Messenger - genetics Sequence Analysis, DNA Tissue Distribution
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Summary:	Juvenile hormones (JH) are a class of regulatory sesquiterpenoids that control metamorphosis in immature insects and reproduction in adult insects. The regulation of JH synthesis by the corpora allata (CA), a pair of endocrine glands with nervous connections to the brain, is achieved by a complex interplay of stimulatory and inhibitory factors mediated in part by the brain. The neuropeptide, allatotropin (Mas AT), was recently isolated and sequenced from the brain of the tobacco hornworm Manduca sexta. Mas AT is a 13-residue amidated peptide that activates JH synthesis in adult, but not larval, lepidopteran CA. A 23-nucleotide degenerate oligonucleotide was designed based on the peptide sequence and was used to isolate the Mas AT genomic clone. The Mas AT gene is expressed as three mRNAs which differ from one another by alternative splicing. These mRNAs are predicted to encode three distinct prohormones, each containing Mas AT. A restriction fragment from the genomic clone was then used to isolate the cDNA clone. In situ hybridization and immunohistochemistry studies show that Mas AT is expressed in both the central and enteric nervous systems. Cells expressing Mas AT were identified in the brain, frontal ganglion and terminal ganglion.
Bibliography:	The novel nucleotide sequence data published here have been submitted to the GenBank sequence data bank and are available under accession numbers U62100, U62101, U62102. Note. ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0014-2956 1432-1033
DOI:	10.1111/j.1432-1033.1996.0588u.x