Tuning Lipase Reaction for Production of Fatty Acids from Oil

Tuning Lipase Reaction for Production of Fatty Acids from Oil

Fats or oils are split partially or completely to obtain fatty acids that find wide applications in oleo-chemical industries. Lipase-mediated complete splitting (hydrolysis) of oils is a green process having great potential to replace the traditional methods of oil splitting. However, cost of lipase...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology Vol. 180; no. 3; pp. 504 - 515
Main Authors: Odaneth, Annamma A., Vadgama, Rajeshkumar N., Bhat, Anuradha D., Lali, Arvind M.
Format: Journal Article
Language:English
Published: New York Springer US 01-10-2016
Springer Nature B.V
Subjects:
Biochemistry
Biotechnology
Catalysis
Chemical reactions
Chemistry
Chemistry and Materials Science
Chromatography, High Pressure Liquid
Fatty acids
Fatty Acids - biosynthesis
Hydrolysis
Lipase - metabolism
Oils & fats
Oils - metabolism
Solvents - chemistry
Triglycerides - metabolism
Water - chemistry
Hydrolysis of oil
Monophasic reaction medium
1,3-Regioselective lipase
Free fatty acid yield
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Summary:Fats or oils are split partially or completely to obtain fatty acids that find wide applications in oleo-chemical industries. Lipase-mediated complete splitting (hydrolysis) of oils is a green process having great potential to replace the traditional methods of oil splitting. However, cost of lipases, mechanistic kinetic equilibrium and associated operational limitations prove to be deterrents for scale up of the enzymatic oil splitting process. In the present study, we demonstrate the use of immobilised 1,3-regioselective lipase (HyLIP) for complete hydrolysis of oil in monophasic reaction medium. Incorporation of a polar organic solvent ( tert -butanol, 1:5, v / v ) homogenises the oil-water mixture and contributes positively towards complete hydrolysis. The monophasic oil hydrolysis reaction with optimised water concentration (0.05 %, v / v ) gave Free Fatty Acid (FFA) yield of 88 % (HyLIP and Novozym-435) and 66 % (TLIM and RMIM). Smart reaction engineering and modification of the reaction intermediates to favourable substrate lead to ∼99 % degree of hydrolysis of triglycerides with ∼90 % FFA yield using 1,3-regioselective lipase. The present work becomes basic platform for developing technologies for synthesis of fatty acids, monoglycerides, diglycerides and glycerol.
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ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-016-2113-9