Assessment of homozygosity in transgenic plants using selectable markers

Production of homozygous transgenic plants is a prerequisite for the phenotypic analysis and/or for the commercial release of transgenic plants for cultivation. Here we present a simple protocol for the selection of homozygous transgenics using antibiotics as a selectable marker. The protocol has be...

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Bibliographic Details
Published in:	STAR protocols Vol. 4; no. 1; p. 102031
Main Authors:	Kammar, Supriya S., Bhat, Ajay R., Hegde, Subrahmanya, Rajulu, Charukesi, Mohan, Thotegowdanapalya C.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 17-03-2023 Elsevier
Subjects:	Genetics Molecular Biology Plant Sciences Protocol Molecular Biology Genetics Plant Sciences
Online Access:	Get full text
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Summary:	Production of homozygous transgenic plants is a prerequisite for the phenotypic analysis and/or for the commercial release of transgenic plants for cultivation. Here we present a simple protocol for the selection of homozygous transgenics using antibiotics as a selectable marker. The protocol has been used to select homozygous rice transgenic plants using hygromycin antibiotic. However, the described protocol can be used for selction of homozygous in any transgenic plants using a appropriate selectable marker. For complete details on the use and execution of this protocol, please refer to Passricha et al. (2016).1 [Display omitted] •Generation of transgenic plants using agrobacterium-mediated transformation•Selection of transformed plants on antibiotic medium•Confirmation of transgenics by using polymerase chain reaction•Identification of stable homozygous transgenic plants Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Production of homozygous transgenic plants is a prerequisite for the phenotypic analysis and/or for the commercial release of transgenic plants for cultivation. Here we present a simple protocol for the selection of homozygous transgenics using antibiotics as a selectable marker. The protocol has been used to select homozygous rice transgenic plants using hygromycin antibiotic. However, the described protocol can be used for selction of homozygous in any transgenic plants using a appropriate selectable marker.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Technical contact: mohantc@jssuni.edu.in These authors contributed equally Lead contact
ISSN:	2666-1667 2666-1667
DOI:	10.1016/j.xpro.2022.102031