Search Results - "Bharathi, Anekella"

Report of the 2019 NIST-FDA workshop on standards for next generation sequencing detection of viral adventitious agents in biologics and biomanufacturing by Cleveland, Megan H., Anekella, Bharathi, Brewer, Michael, Chin, Pei-Ju, Couch, Heather, Delwart, Eric, Huggett, Jim, Jackson, Scott, Martin, Javier, Monpoeho, Serge, Morrison, Tom, Ng, Siemon H.S., Ussery, David, Khan, Arifa S.

“…Adventitious virus testing assures product safety by demonstrating the absence of viruses that could be unintentionally introduced during the manufacturing…”
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Abstract 471: Reference materials for tumor mutational burden transcriptome profiling by Butler, Matthew G., Konigshofer, Yves, Dickens, Jessica, Anekella, Bharathi

“…Immune Checkpoint Inhibitor (ICI) therapies continue to revolutionize treatment of advanced malignancies. However, identification of individuals who respond to…”
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A Mutation in the Schizosaccharomyces pombe rae1 Gene Causes Defects in Poly(A)+ RNA Export and in the Cytoskeleton (∗) by Brown, Julie A., Bharathi, Anekella, Ghosh, Anil, Whalen, William, Fitzgerald, Ellen, Dhar, Ravi

“…A collection of fission yeast Schizosaccharomyces pombe conditional mutants was screened for defective nucleocytoplasmic transport of poly(A)+ RNA by…”
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Abstract 2232: Optimizing Performance of Whole Transcriptome RNA-Seq Reference by Huang, Catherine, Butler, Matthew G., Konigshofer, Yves, Dickens, Jessica, Vemula, Rajeswari, Clement, Omoshile, Anekella, Bharathi

“…Purpose: Whole transcriptome RNA-sequencing (RNA-Seq) has emerged as one of the most effective methods for detection of genomic rearrangements in cancer…”
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Abstract 1385: Patient-derived xenografts as validation material for NGS assays: Analysis of genetic homogeneity by Konigshofer, Yves, Anekella, Bharathi, Garlick, Russell

“…Full process controls and validation materials for next generation sequencing (NGS)-based molecular diagnostics in Oncology that mimic a biopsy sample and are…”
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Abstract 1982: Development of reference material for blood tumor mutational burden measurement by Butler, Matthew G., Konigshofer, Yves, Clement, Omoshile, Anfora, Andrew, Brudzewsky, Dan, Anekella, Bharathi, Garlick, Russell

“…Tumor mutational burden (TMB) is a measurement of the somatic mutations acquired by a tumor genome that cause nonsynonymous changes in proteins. TMB is a…”
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Abstract 1322: Development and performance of a formalin-damaged multiplexed DNA tumor mutation FFPE reference material by Lowe, Dana J. Ruminski, Philkana, Deepika, Huang, Catherine, Butler, Matthew G., Clement, Omoshile, Anekella, Bharathi

“…Tumor profiling often begins with a tissue biopsy that is formalin fixed and paraffin embedded (FFPE), a process that introduces various kinds of damage in the…”
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Abstract 3394: Controls to detect limit of detection for BRAF V600E as an actionable mutation of interest by NextGen Sequencing and qPCR by Chisholm, Cristine L., Garlick, Russell, Anekella, Bharathi

“…Background: Molecularly targeted therapy across tumor types has great potential to benefit cancer patients. In order to validate the assays being used to…”
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Abstract 738: Highly multiplexed and precisely calibrated reference materials for copy number variation detection by Huang, Catherine, Konigshofer, Yves, Dickens, Jessica, Anekella, Bharathi

“…Introduction: Genomic instability is a hallmark of cancer, and copy number variation (CNV), including gene duplication and deletion, can be a key driver of…”
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The human RAE1 gene is a functional homologue of Schizosaccharomyces pombe rae1 gene involved in nuclear export of Poly(A) + RNA by Bharathi, Anekella, Ghosh, Anil, A. Whalen, William, Ho Yoon, Jin, Pu, Robert, Dasso, Mary, Dhar, Ravi

“…A Schizosaccharomyces pombe temperature-sensitive mutant, rae1-1, was previously identified by us as being defective in nuclear export of Poly(A) + RNA when…”
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Abstract 1709: Consistent performance of highly multiplexed RNA fusion reference materials across different NGS assays in a multi-lab study by Lowe, Dana J. Ruminski, Philkana, Deepika, Huang, Catherine, Clement, Omoshile, Anfora, Andrew, Brudzewsky, Dan, Anekella, Bharathi

“…Introduction: Gene fusions, usually the result of chromosomal rearrangements, are frequently associated with many cancer types, and hence clinically…”
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Abstract 3167: Improving and standardizing TMB assay performance by Butler, Matthew G., Konigshofer, Yves, Nguyen, Lequan, Kalotra, Shikha, Clement, Omo, Garlick, Russell K., Anekella, Bharathi

“…Determining and using the most effective and safest treatment is of great importance in cancer disease management. Checkpoint inhibitors that target immune…”
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Abstract 1364: A Non-Small Cell Lung Cancer Reference Material by Tomson, Farol L., Huang, Catherine, Clement, Omo, Garlick, Russell, Anekella, Bharathi

“…Lung cancer is the leading cause of cancer death in the United States as well as globally. The major diagnosis (~85%) of lung cancer patients is non-small cell…”
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Abstract 1978: Reference materials for measurable residual disease (MRD) monitoring by Konigshofer, Yves, Butler, Matthew G., Dickens, Jessica, Clement, Omoshile, Anfora, Andrew, Brudzewsky, Dan, Anekella, Bharathi, Garlick, Russell K.

“…Here we describe reference materials for measurable residual disease (MRD) assay design and validation as well as initial data from testing. The analytical…”
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Abstract 4315: NGS-based reference materials for fusion and somatic variant detection in myeloid cancers by Tomson, Farol Lovell, Kamineni, Praveena, Huang, Catherine, Dickens, Jessica, Brudzewsky, Dan, Clement, Omoshile, Santhanam, Ram, Anekella, Bharathi

“…The large range of genetic aberrations and genes involved Myeloid malignancies, clonal diseases of hematopoietic progenitor cells which can lead to…”
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Abstract 3961: Methodological considerations in the preparation of biomimetic reference materials for ctDNA assays by Harkins, Seth B., Tomson, Farol L., Anekella, Bharathi, Garlick, Russell

“…Clinical utilization of cell-free DNA (cfDNA) has become commonplace for NIPS testing and has recently been more widely deployed to quantitate cell-free…”
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Abstract 4758: Use of highly multiplexed reference materials to facilitate validation of a clinical NGS tumor fusion RNA assay by Huang, Catherine, Barua, Subit, Philkana, Deepika, Garlick, Russell, Anekella, Bharathi, Fernandes, Helen

“…Introduction: Next Generation Sequencing assays for detection of tumor RNA fusions must undergo rigorous validation before clinical implementation. Validations…”
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Abstract 1421: Flexible tools for the development and performance verification of customized target enrichment panels by Hendrickson, Cynthia L., Mauceli, Evan, Barry, Andrew, Patel, Kruti, Huang, Catherine, Adams, Scott, Anekella, Bharathi

“…Implementation of next generation sequencing for variant identification and discovery presents difficulties both in the selection of genomic loci for inclusion…”
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Abstract 4540: Development of well characterized breast, lung, and brain cancer copy number variation reference materials by Lowe, Dana Ruminski, Dickens, Jessica, Huang, Catherine, Konigshofer, Yves, Anekella, Bharathi

“…Introduction: Copy number variation (CNV), including gene amplification and deletion, can be a key driver of oncogenesis. Pathogenic CNVs are often associated…”
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Abstract 1627: Custom VariantFlex technology allows rapid design and testing of reference materials for tumor genotyping assays by Huang, Catherine, Santhanam, Ram, Brudzewsky, Dan, Brown, Trevor, Garlick, Russell, Anekella, Bharathi

“…Introduction: Development and validation of tumor genotyping assays requires highly characterized reference materials for effective assessment of accuracy,…”
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