Differential expression patterns of N-acetylglucosaminyl transferases and polylactosamines in uterine lesions

Polylactosamine (polyLacNAc) is a fundamental structure in glycoconjugates and it is expressed in specific cells/tissues associated with the development and carcinogenesis. β1,3-N-acetylglucosaminyl transferases (β3GnTs) play an important role in polyLacNAc synthesis, however the roles of these glyc...

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Published in:	European journal of histochemistry Vol. 58; no. 2; p. 2334
Main Authors:	Clark, A T R, Guimarães da Costa, V M L, Bandeira Costa, L, Bezerra Cavalcanti, C L, De Melo Rêgo, M J B, Beltrão, E I C
Format:	Journal Article
Language:	English
Published:	Italy PAGEPress Publications 26-05-2014 PAGEPress Publications, Pavia, Italy
Subjects:	Adult Aged Aged, 80 and over Amino Sugars - biosynthesis cancer, lectin, N-acetylglucosaminyltransferase, poly-N-acetyllactosamine Carcinoma, Squamous Cell - enzymology Carcinoma, Squamous Cell - pathology Cervical Intraepithelial Neoplasia - enzymology Cervical Intraepithelial Neoplasia - pathology Female Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Humans Middle Aged N-Acetylglucosaminyltransferases - biosynthesis Neoplasm Proteins - biosynthesis Original Paper Polysaccharides - biosynthesis Uterine Cervical Neoplasms - enzymology Uterine Cervical Neoplasms - pathology
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Summary:	Polylactosamine (polyLacNAc) is a fundamental structure in glycoconjugates and it is expressed in specific cells/tissues associated with the development and carcinogenesis. β1,3-N-acetylglucosaminyl transferases (β3GnTs) play an important role in polyLacNAc synthesis, however the roles of these glycosyltransferases and their products in cancer progression are still unclear. In this sense, this work aimed to evaluate differential expression pattern of the N-acetylglucosaminyl transferases and polylactosamines in invasive and premalignant lesions of the uterus cervix. The expression of β3GnT2 and β3GnT3 were evaluated in normal (n=10) and uterine cervix lesions (n= 120) malignant (squamous carcinoma - SC) and premalignant (cervical intraepithelial neoplasia - CIN - grades 1, 2 and 3) using immunohistochemistry. Besides, lectin histochemistry with Phytolacca americana lectin (PWM) and Wheat germ agglutinin (WGA) was also carried out to observe the presence of polyLacNAc chains and N-acetylglucosamine (GlcNAc), respectively. The β3GnT3 was expressed in almost all samples (99%) and β3GnT2 was higher expressed in disease samples mainly in CIN 3, when compared with normal (P=0.002), CIN 1 (P=0.009) and CIN 2 (P=0.03). The expression of polyLacNAc was higher is SC samples, when compared with normal (P=0.03), CIN 1 (P=0.02) and CIN 3 (P=0.004), and was observed only nuclear expression in nearly 50% of the SC samples, showing a statistically significant when compared with normal (P=0.01), CIN 1 (P=0.002), CIN 2 (P=0.007) and CIN 3 (P=0.04). Deferring from transferases and polyLacNAc chains, GlcNAc (WGA ligand) reveals a gradual staining pattern decrease with the increase of the lesion degree, being more expressed in CIN 1 lesions when compared with normal (P<0.0001), CIN 2 (P<0.0001), SC (P<0.0001) and CIN 3 (P=0.0003). Our data reveals β3GnT2 and polyLacNAc may be involved in the progression of the pre-malignant lesions of human the uterine cervix. In addition, polyLacNAc expression only in the nucleus can be associated a poor prognostic in uterine lesions.
ISSN:	1121-760X 2038-8306
DOI:	10.4081/ejh.2014.2334