Effect of Diluents and Storage Time on the Cryopreservation of Collared Peccary ( Pecari tajacu ) Semen after Cooling Storage in a Transport Container at 5 °C

Effect of Diluents and Storage Time on the Cryopreservation of Collared Peccary ( Pecari tajacu ) Semen after Cooling Storage in a Transport Container at 5 °C

We verified the possibility of cooling peccary semen for 4, 24, and 48 h before cryopreservation, using different dilution media (TRIS + egg yolk (20%) and PRIMXcell Ultra). Ten ejaculates were divided equally into six aliquots and then diluted. Two aliquots were stored in a biological incubator (4...

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Published in:Animals (Basel) Vol. 14; no. 6; p. 934
Main Authors: Santos, Romário P, Silva, Andréia M, Pereira, Ana G, Cavalcante, Yasmim C S, Matos, Yuri G, Bezerra, Gabriel S C, Dantas, Lilian L, Silva, Alexandre R
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 18-03-2024
MDPI
Subjects:
Animals
biobanking
Biobanks
Botutainer
Cooling
Cryopreservation
Excipients
Glycerol
Males
Morphology
PRIMXcell Ultra
semen transport
Sperm
tayassuids
Brazil
France
United States > US
tayassuids
Botutainer
semen transport
biobanking
PRIMXcell Ultra
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Summary:We verified the possibility of cooling peccary semen for 4, 24, and 48 h before cryopreservation, using different dilution media (TRIS + egg yolk (20%) and PRIMXcell Ultra). Ten ejaculates were divided equally into six aliquots and then diluted. Two aliquots were stored in a biological incubator (4 h), and the remaining aliquots were stored in a commercial container, the Botutainer (24 and 48 h), both at 5 °C. The samples were cryopreserved and then evaluated for kinetic parameters, functionality, integrity, mitochondrial activity, morphology, and sperm binding capacity. After thawing, samples diluted in TRIS showed total motility of 43.4 ± 6.8%, 48.4 ± 6.2%, and 38.6 ± 5.0% after cooling for 4, 24, and 48 h before cryopreservation, respectively. Such results are significantly greater than those achieved with the use of PRIMXcell diluent for 4 (8.3 ± 2.8%), 24 (4.7 ± 1.4%), and 48 h (4.8 ± 2.9%) storage ( < 0.05). Furthermore, TRIS provided better preservation of sperm membrane integrity when samples were cooled for 24 h (44.5 ± 4.7%) before cryopreservation compared to those samples diluted in PRIMXcell Ultra stored for 24 (25.7 ± 4.0%) and 48 h (25.2 ± 4.0%) before freezing ( < 0.05). In summary, we suggest TRIS diluent + egg yolk (20%) as an effective option to allow semen to cool for 24 or 48 h in a transport container before cryopreservation.
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ISSN:2076-2615
2076-2615
DOI:10.3390/ani14060934