Fungal contaminants observed during micropropagation of Lilium candidum L. and the effect of chemotherapeutic substances applied after sterilization

Fungal contaminants observed during micropropagation of Lilium candidum L. and the effect of chemotherapeutic substances applied after sterilization

Lilium candidum L. is a species which grows in the South West Anatolia region of Turkey. It is a bulbous plant with beautifully scented flowers and is used in the floral industry. The bulbs are produced by using traditional propagation and in vitro techniques. Micropropagation is a rapid propagation...

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Bibliographic Details
Published in:African journal of biotechnology Vol. 9; no. 7; pp. 991 - 995
Main Authors: Filiz, Altan, Betuuml l, Buuml ruuml n, Nurettin, Şahin
Format: Journal Article
Language:English
Published: 15-02-2010
Subjects:
Alternaria
Antibiotics
Aspergillus
Benomyl
Bulbs
Contaminants
Contamination
Cultural characteristics
Cylindrocarpon
Explants
Flowers
Fungi
Fungicides
Fusarium
Light effects
Lilium
micropropagation
Nystatin
Penicillin
Penicillium
Propagation
Rhizopus
Sterilization
Streptomycin
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Summary:Lilium candidum L. is a species which grows in the South West Anatolia region of Turkey. It is a bulbous plant with beautifully scented flowers and is used in the floral industry. The bulbs are produced by using traditional propagation and in vitro techniques. Micropropagation is a rapid propagation technique, but the greatest problem is contamination with fungi and bacteria. Antibiotic and fungicide treatments were done after sterilization for micropropagation of L. candidum. Fungal contaminants formed during the culture were determined. Bulb scales were used as explants (5 - 10 mm width) and were cultured in photoperiodic conditions (16 h light, 8 h dark) or complete darkness. Bulb scales rinsed in water were surface sterilized, then solutions containing chemotherapeutic substances (Benomyl, Nystatin, Streptomycin, Penicillin) in different combinations were applied for 30 min and subsequently were cultured in MS medium with supplement 0.1 mg dm super(-3) NAA + 0.01 mg dm super(-3) BA. During the experiment, fungal contaminants were observed in full treatments. Determined contaminants were identified according to their morphological and cultural characteristics by cultivation and were comprised of: Fusarium, Penicillium, Alternaria, Rhizopus, Cylindrocarpon and Aspergillus species. The most effective treatment against fungal contaminations was achieved by utilizing a Benomyl (100 mg dm super(-3)) + Nystatin (100 mg dm super(-3)) treatment combination.
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ISSN:1684-5315
1684-5315
DOI:10.5897/AJB08.090