In vitro anti-bacterial and biological properties of magnetron co-sputtered silver-containing hydroxyapatite coating

In vitro anti-bacterial and biological properties of magnetron co-sputtered silver-containing hydroxyapatite coating

Bacterial infection after implant placement is a significant rising complication. In order to reduce the incidence of implant-associated infections, several biomaterial surface treatments have been proposed. In this study, the effect of in vitro antibacterial activity and in vitro cytotoxicity of co...

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Bibliographic Details
Published in:Biomaterials Vol. 27; no. 32; pp. 5512 - 5517
Main Authors: Chen, W., Liu, Y., Courtney, H.S, Bettenga, M., Agrawal, C.M., Bumgardner, J.D., Ong, J.L.
Format: Journal Article
Language:English
Published: Netherlands Elsevier Ltd 01-11-2006
Subjects:
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Bacterial adhesion
Cell Line
Cell Survival - drug effects
Cytotoxicity
Durapatite - chemistry
Durapatite - pharmacology
Durapatite - toxicity
Humans
Hydroxyapatite coating
Materials Testing
Silver
Silver - chemistry
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus epidermidis
Staphylococcus epidermidis - drug effects
X-Ray Diffraction
Silver
Cytotoxicity
Bacterial adhesion
Hydroxyapatite coating
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Summary:Bacterial infection after implant placement is a significant rising complication. In order to reduce the incidence of implant-associated infections, several biomaterial surface treatments have been proposed. In this study, the effect of in vitro antibacterial activity and in vitro cytotoxicity of co-sputtered silver (Ag)-containing hydroxyapatite (HA) coating was evaluated. Deposition was achieved by a concurrent supply of 10 W to the Ag target and 300 W to the HA target. Heat treatment at 400 °C for 4 h was performed after 3 h deposition. X-ray diffraction, contact angles measurements, and surface roughness were used to characterize the coating surfaces. The RP12 strain of Staphylococcus epidermidis (ATCC 35984) and the Cowan I strain of Staphylococcus aureus were used to evaluate the antibacterial activity of the Ag-HA coatings, whereas human embryonic palatal mesenchyme cells, an osteoblast precursor cell line, were used to evaluate the in vitro cytotoxicity of the coatings. X-ray diffraction analysis performed in this study indicated peaks corresponding to Ag and HA on the co-sputtered Ag-HA surfaces. The contact angles for HA and Ag-HA surfaces were observed to be significantly lower when compared to Ti surfaces, whereas no significant difference in surface roughness was observed for all groups. In vitro bacterial adhesion study indicated a significantly reduced number of S. epidermidis and S. aureus on Ag-HA surface when compared to titanium (Ti) and HA surfaces. In addition, no significant difference in the in vitro cytotoxicty was observed between HA and Ag-HA surfaces. Overall, it was concluded that the creation of a multifunctional surface can be achieved by co-sputtering the osteoconductive HA with antibacterial Ag.
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ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2006.07.003