Antimicrobial Contribution of Chitosan Surface-Modified Nanoliposomes Combined with Colistin against Sensitive and Colistin-Resistant Clinical Pseudomonas aeruginosa

Antimicrobial Contribution of Chitosan Surface-Modified Nanoliposomes Combined with Colistin against Sensitive and Colistin-Resistant Clinical Pseudomonas aeruginosa

Colistin is a re-emergent antibiotic peptide used as a last resort in clinical practice to overcome multi-drug resistant (MDR) Gram-negative bacterial infections. Unfortunately, the dissemination of colistin-resistant strains has increased in recent years and is considered a public health problem wo...

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Published in:Pharmaceutics Vol. 13; no. 1; p. 41
Main Authors: Laverde-Rojas, Valentina, Liscano, Yamil, Rivera-Sánchez, Sandra Patricia, Ocampo-Ibáñez, Ivan Darío, Betancourt, Yeiston, Alhajj, Maria José, Yarce, Cristhian J, Salamanca, Constain H, Oñate-Garzón, Jose
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 30-12-2020
MDPI
Subjects:
Antibiotics
Antimicrobial agents
Aqueous solutions
Bacterial infections
chitosan
colistin
Laboratories
Lipids
MDR-Bacteria
nanoliposomes
Particle size
Peptides
Polymers
Public health
Staphylococcus infections
United States > US
Germany
chitosan
colistin
MDR-Bacteria
nanoliposomes
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Summary:Colistin is a re-emergent antibiotic peptide used as a last resort in clinical practice to overcome multi-drug resistant (MDR) Gram-negative bacterial infections. Unfortunately, the dissemination of colistin-resistant strains has increased in recent years and is considered a public health problem worldwide. Strategies to reduce resistance to antibiotics such as nanotechnology have been applied successfully. In this work, colistin was characterized physicochemically by surface tension measurements. Subsequently, nanoliposomes coated with highly deacetylated chitosan were prepared with and without colistin. The nanoliposomes were characterized using dynamic light scattering and zeta potential measurements. Both physicochemical parameters fluctuated relatively to the addition of colistin and/or polymer. The antimicrobial activity of formulations increased by four-fold against clinical isolates of susceptible but did not have antimicrobial activity against multidrug-resistant (MDR) bacteria. Interestingly, the free coated nanoliposomes exhibited the same antibacterial activity in both sensitive and MDR strains. Finally, the interaction of colistin with phospholipids was characterized using molecular dynamics (MD) simulations and determined that colistin is weakly associated with micelles constituted by zwitterionic phospholipids.
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ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics13010041