Detection of Serum Allergen-Specific IgE in Atopic Dogs Tested in Northern Italy: Preliminary Study

Canine atopic dermatitis (CAD) is a pruritic allergic skin disease associated with IgE-mediated hypersensitivity. IgE is detected using Serum Allergen-Specific IgE test (SAT) in order to identify allergens. The present study aims to identify the environmental allergens in atopic dogs living in North...

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Bibliographic Details
Published in:	Animals (Basel) Vol. 11; no. 2; p. 358
Main Authors:	Di Tommaso, Morena, Luciani, Alessia, Crisi, Paolo Emidio, Beschi, Marica, Rosi, Paolo, Rocconi, Francesca, Miglio, Arianna
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 01-02-2021 MDPI
Subjects:	Allergens Allergies allergy testing Antigens Atopic dermatitis Clinical medicine Dermatitis dog Dogs Enzyme-linked immunosorbent assay Enzymes House dust Hypersensitivity IgE Immunoglobulin E Immunotherapy Indoor air pollution Indoor environments Laboratories Monoclonal antibodies Northern Italy Pollen Rumex acetosa serology Skin diseases Software United States > US Italy Northern Italy atopic dermatitis allergy testing dog IgE serology
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Summary:	Canine atopic dermatitis (CAD) is a pruritic allergic skin disease associated with IgE-mediated hypersensitivity. IgE is detected using Serum Allergen-Specific IgE test (SAT) in order to identify allergens. The present study aims to identify the environmental allergens in atopic dogs living in Northern Italy using SAT. The screening SAT (sSAT), using a monoclonal antibody cocktail-based ELISA to identify indoor and outdoor allergens, was performed. In all positive samples, an anti-IgE monoclonal antibody ELISA test was performed to extend panel of allergens. Out of 117 selected dogs, 69 were included in the study; 71% were positive and 29% were negative to sSAT. Among the 49 positive sSAT, 53% were positive for both indoor and outdoor, 38.8% only for indoor, and 8.2% only for outdoor allergens. This is the first study on the frequency of allergens involved in CAD in Italy using SAT. IgE hypersensitivity in atopic dogs of Northern Italy is usually associated with indoor allergens, primarily house dust mites. Among the outdoor allergens, an important role was played by . Polysensitization also commonly occurs. Therefore, since the numerous factors affect the IgE positivity in CAD, specific panels for geographical areas should be considered and re-evaluated at time intervals.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to the work.
ISSN:	2076-2615 2076-2615
DOI:	10.3390/ani11020358