Simultaneous determination of cefepime, vancomycin and imipenem in human plasma of burn patients by high-performance liquid chromatography

A liquid chromatographic method with UV detection for simultaneous determination of cefepime, vancomycin and imipenem has been developed. Cefuroxime was used as internal standard. After the clean up of samples by plasma protein precipitation, 5 μl of the extract were injected into the chromatograph...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 860; no. 2; pp. 241 - 245
Main Authors: López, K.J. Vera, Bertoluci, D. Faria, Vicente, K.M., Dell’Aquilla, A.M., Santos, S.R.C. Jorge
Format: Journal Article
Language:English
Published: Amsterdam Elsevier B.V 15-12-2007
Elsevier Science
Subjects:
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A liquid chromatographic method with UV detection for simultaneous determination of cefepime, vancomycin and imipenem has been developed. Cefuroxime was used as internal standard. After the clean up of samples by plasma protein precipitation, 5 μl of the extract were injected into the chromatograph and peaks were eluted from the Sulpelcosil™ LC-18 column using a mobile phase consisting of 0.075 M acetate buffer:acetonitrile (92:8, v/v), pH 5.0 at low rate (0.8 ml/min). The detection wavelength was 230 nm. The limit of detection was 0.4 μg/ml for cefepime and 0.2 μg/ml for vancomycin and imipenem. The method was applied to plasma samples of burn patients, and only small volumes of plasma were required for the simultaneous determination of those antimicrobial agents.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2007.10.041