Microscopic diagnosis of the leaf and stem of Piper solmsianum C.DC

Piper solmsianum C.DC., which is popularly known as pariparoba, is a shrub that measures 1–3 m in height and it inhabits areas with wet tropical soils. The objective of this study was to analyze the leaf and stem anatomy using light microscopy, scanning electron micrographs, and energy‐dispersive X‐...

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Published in:Microscopy research and technique Vol. 80; no. 8; pp. 831 - 837
Main Authors: Bertocco, A. R. P., Migacz, I. P., Santos, V. L. P., Franco, C. R. C., Silva, R. Z., Yunes, R. A., Cechinel‐Filho, V., Budel, J. M.
Format: Journal Article
Language:English
Published: United States Wiley Subscription Services, Inc 01-08-2017
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Summary:Piper solmsianum C.DC., which is popularly known as pariparoba, is a shrub that measures 1–3 m in height and it inhabits areas with wet tropical soils. The objective of this study was to analyze the leaf and stem anatomy using light microscopy, scanning electron micrographs, and energy‐dispersive X‐ray spectroscopy in order to provide information for species identification. The anatomical profile showed the following main microscopic markers: hypostomatic leaf; hypodermis layer on both sides; pearl glands; biconvex midrib shape; five collateral vascular bundles in open arc with the central bundle larger than the others; circular stem shape; collateral vascular bundles arranged in two rings; sinuous sclerenchymatic sheath in the pith; secretory idioblasts; and starch grains in the mesophyll, in the ground parenchyma of the midrib, petiole, and in the stem; and six morphotypes of calcium oxalate crystals (styloids, cuneiform, tabular crystal rosettes, cuneiform crystal rosettes, elongated square dipyramids, as well as very elongated square dipyramids). The main microscopic markers are pearl glands; biconvex midrib and circular petiole and stem shape; secretory idioblasts and six morphotypes of calcium oxalate crystals.
Bibliography:Review Editor: Prof. Alberto Diaspro
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ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.22870