Search Results - "Bertheleme, Nicolas"

Unlocking the secrets of the gatekeeper: Methods for stabilizing and crystallizing GPCRs by Bertheleme, Nicolas, Chae, Pil Seok, Singh, Shweta, Mossakowska, Danuta, Hann, Michael M., Smith, Kathrine J., Hubbard, Julia A., Dowell, Simon J., Byrne, Bernadette

“…G-protein coupled receptors (GPCRs) are integral membrane cell surface receptors with key roles in mediating the cellular responses to a wide range of…”
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Arginine 199 and leucine 208 have key roles in the control of adenosine A2A receptor signalling function by Bertheleme, Nicolas, Strege, Annette, Bunting, Sorrel E, Dowell, Simon J, Byrne, Bernadette

“…One successful approach to obtaining high-resolution crystal structures of G-protein coupled receptors is the introduction of thermostabilising mutations…”
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Heterologous expression of G-protein-coupled receptors in yeast by Bertheleme, Nicolas, Singh, Shweta, Dowell, Simon, Byrne, Bernadette

“…Heterologous yeast expression systems have been successfully used for the production of G-protein-coupled receptors (GPCRs) for both structural and functional…”
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Loss of constitutive activity is correlated with increased thermostability of the human adenosine A2A receptor by Bertheleme, Nicolas, Singh, Shweta, Dowell, Simon J, Hubbard, Julia, Byrne, Bernadette

“…Background and Purpose Thermostabilization by mutagenesis is one method which has facilitated the determination of high‐resolution structures of the adenosine…”
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Production of stable membrane proteins for the development of biologics by Duclos, Stephanie, Bertheleme, Nicolas, Yadav, Rahul, Hitchman, Richard, Singh, Shweta, Barker, John, Ullmann, Dirk

“…Abstract only…”
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Arginine 199 and Leucine 208 Have Key Roles in the Control of Adenosine A.sub.2A Receptor Signalling Function by Bertheleme, Nicolas, Strege, Annette, Bunting, Sorrel E, Dowell, Simon J, Byrne, Bernadette

“…One successful approach to obtaining high-resolution crystal structures of G-protein coupled receptors is the introduction of thermostabilising mutations…”
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Loss of constitutive activity is correlated with increased thermostability of the human adenosine A 2A receptor by Bertheleme, Nicolas, Singh, Shweta, Dowell, Simon J, Hubbard, Julia, Byrne, Bernadette

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Functional characterisation of a2a receptor thermostable mutants using a yeast signalling assay by Bertheleme, Nicolas

“…G-protein-coupled receptors (GPCR) are transmembrane proteins that play a crucial role in the communication of cells with their external environment. In the…”
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The identification a novel, selective, non-steroidal, functional glucocorticoid receptor antagonist by Rimland, Joseph, Dunne, Angela, Hunjan, Suchete S., Sasse, Rosemary, Uings, Iain, Montanari, Dino, Caivano, Matilde, Shah, Poonam, Standing, David, Gray, David, Brown, David, Cairns, William, Trump, Ryan, Smith, Paul W., Bertheleme, Nicolas, D’Alessandro, Pier, Gul, Sheraz, Vimal, Mythily, Smith, David N., Watson, Stephen P.

“…The identification of novel, potent, non-steroidal/small molecule functional GR antagonist GSK1564023A PR is described. Associated structure–activity…”
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Radioligand binding analysis as a tool for quality control of GPCR production for structural characterization: adenosine A(2a)R as a template for study by Singh, Shweta, Zhang, Minghao, Bertheleme, Nicolas, Kara, Elodie, Strange, Philip G, Byrne, Bernadette

“…Functional characterization of G protein-coupled receptors is essential to ascertain the suitability of a protein target for downstream studies and to help…”
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Purification of the human G protein-coupled receptor adenosine A(2a)R in a stable and functional form expressed in Pichia pastoris by Singh, Shweta, Zhang, Minghao, Bertheleme, Nicolas, Strange, Philip G, Byrne, Bernadette

“…The isolation of membrane proteins with the aim of producing highly pure, homogeneous, stable, and functional material remains challenging, and it is often…”
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