Expression of glutamine synthetase during the anaerobic germination of Oryza sativa L

Glutamine synthetase (GS; EC.6.3.1.2.) occurs as cytosolic (GS1) and plastidic (GS2) polypeptides. This paper describes the expression of GS isoenzymes in coleoptile during the anaerobic germination of rice (Oryza sativa L.) and the influence of exogenous nitrate on this. By immunoprecipitation with...

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Bibliographic Details
Published in:	Planta Vol. 195; no. 1; pp. 147 - 149
Main Authors:	Mattana, M. (CNR, Milano (Italy). Ist. Biosintesi Vegetali), Bertani, Y, Reggiani, R
Format:	Journal Article
Language:	English
Published:	Berlin Springer-Verlag 01-11-1994 Springer
Subjects:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Agronomy. Soil science and plant productions Ammonia ANOXIA ANOXIE Antiserum Biological and medical sciences COLEOPTILE COLEOPTILES COLEOPTILOS Economic plant physiology Enzym ENZYMIC ACTIVITY Fundamental and applied biological sciences. Psychology Genexpression GERMINACION GERMINATION GLUTAMATE AMMONIA LIGASE GLUTAMINA SINTETASA GLUTAMINE SYNTHETASE Glutaminsynthetase GRAINE Immunoprecipitation Koleoptyle Luftmangel Metabolism Nitrates Nitrogen metabolism Nitrogen metabolism and other ones (excepting carbon metabolism) Nutrition. Photosynthesis. Respiration. Metabolism Oryza ORYZA SATIVA Plant cells Plant physiology and development Plants Rapid communication Rice Samenkeimung SEEDS SEMILLA Monocotyledones Carbon-nitrogen ligases Enzyme Isozyme Germination Nitrates Gene expression Cereal crop Oryza sativa Messenger RNA Gramineae Ligases Anaerobe Angiospermae Coleoptile Spermatophyta Glutamate-ammonia ligase
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Summary:	Glutamine synthetase (GS; EC.6.3.1.2.) occurs as cytosolic (GS1) and plastidic (GS2) polypeptides. This paper describes the expression of GS isoenzymes in coleoptile during the anaerobic germination of rice (Oryza sativa L.) and the influence of exogenous nitrate on this. By immunoprecipitation with anti-GS serum, two polypeptides of 41- and 44-kDa were detected of which the former was predominant. After fractionation by ion-exchange chromatography, the 41 and 44 kDa bands were identified as GS1 and GS2, respectively. Northern blot analysis with specific probes showed the presence of mRNA for cytosolic GS but not for the plastidic form. The presence of exogenous nitrate did not alter the activity and expression of GS in the coleoptile. The role of GS during the anaerobic germination of rice seems to induce the re-assimilation of ammonia rather than the assimilation of nitrate.
Bibliography:	94G1263 F62
ISSN:	0032-0935 1432-2048
DOI:	10.1007/BF00206303