OFF bipolar cell density varies by subtype, eccentricity, and along the dorsal ventral axis in the mouse retina

The neural retina is organized along central‐peripheral, dorsal‐ventral, and laminar planes. Cellular density and distributions vary along the central‐peripheral and dorsal‐ventral axis in species including primates, mice, fish, and birds. Differential distribution of cell types within the retina is...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of comparative neurology (1911) Vol. 529; no. 8; pp. 1911 - 1925
Main Authors:	Camerino, Michael J., Engerbretson, Ian J., Fife, Parker A., Reynolds, Nathan B., Berria, Mikel H., Doyle, Jamie R., Clemons, Mellisa R., Gencarella, Michael D., Borghuis, Bart G., Fuerst, Peter G.
Format:	Journal Article
Language:	English
Published:	Hoboken, USA John Wiley & Sons, Inc 01-06-2021 Wiley Subscription Services, Inc
Subjects:	Animal models Animals axon Bipolar cells Cell density dendrite development genetics Glaucoma Horizontal cells Macular degeneration Mice neural neuron Retina Retinal Bipolar Cells - cytology vision vision development genetics neural neuron dendrite axon
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	The neural retina is organized along central‐peripheral, dorsal‐ventral, and laminar planes. Cellular density and distributions vary along the central‐peripheral and dorsal‐ventral axis in species including primates, mice, fish, and birds. Differential distribution of cell types within the retina is associated with sensitivity to different types of damage that underpin major retinal diseases, including macular degeneration and glaucoma. Normal variation in retinal distribution remains unreported for multiple cell types in widely used research models, including mouse. Here we map the distribution of all known OFF bipolar cell (BC) populations and horizontal cells. We report significant variation in the distribution of OFF BC populations and horizontal cells along the dorsal‐ventral and central‐peripheral axes of the retina. Distribution patterns are much more pronounced for some populations of OFF BC cells than others and may correspond to the cell type's specialized functions. Density of OFF bipolar cells vary by subtype, eccentricity and along the dorsal ventral axis. (a) Retinas were disected into four quadrants labeled with short‐wave opsin to determine orientation. (b) Cells were marked and saved as series of individual coordinates which underwent Voronoi tessellation to analyze spatial heterogeneity. (c) Volumetric datasets were counted do determine cell count. (d) OFF‐bipolar cell density contour maps by subtype 1a, 1b, 2, 3a, 3b, 4.
Bibliography:	Funding information Idaho INBRE, Grant/Award Number: P20GM103408; National Eye Institute, Grant/Award Number: EY028297 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 AUTHOR CONTRIBUTIONS All authors had full access to all the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis. Study concept and design: M.J.C and P.G.F. Acquisition of data: M.J.C, I.J.E, N.B.R., M.R.C., M.H.B., P.A.F., J.R.D., and M.D.G. Analysis and interpretation of data: M.J.C and P.G.F. Drafting of the manuscript: M.J.C and P.G.F. Critical revision of the manuscript for important intellectual content: M.J.C. and P.G.F. Statistical analysis: M.J.C and P.G.F. Obtained funding: P.G.F. and B.G.B. Administrative, technical, and material support: P.G.F. Study supervision: P.G.F.
ISSN:	0021-9967 1096-9861
DOI:	10.1002/cne.25064