Investigation of biota associated and natural infection by sporulated bacteria in Culicidae larvae from São Paulo state, Brazil

11,992 of Culex spp. and Aedes spp. larvae were collected, of which 10,321 larvae did not exhibit any apparent symptoms and 1671 exhibited some type of symptom that could not be attributed to a particular pathogen. [Display omitted] ► The aim was to investigate naturally occurring bacteria from Culi...

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Bibliographic Details
Published in:	Journal of invertebrate pathology Vol. 107; no. 1; pp. 11 - 15
Main Authors:	Araújo-Coutinho, Carlos José P.C., Bernardino, Thaissa C., Pires, Eder S., Esch, Lygia V.S., Viviani, Andrea B.P., Cavados, Clara F.G.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier Inc 01-05-2011 Elsevier
Subjects:	Animal, plant and microbial ecology Animals Bacillus - classification Bacillus - isolation & purification Bacillus cereus Bacillus thuringiensis bacteria bioactive properties Biological and medical sciences Brazil Culicidae Culicidae - microbiology Culicidae larvae and bacterial biodiversity DNA electrophoresis enterotoxins Fundamental and applied biological sciences. Psychology heat treatment isozymes Larva larvae Lysinibacillus sphaericus Microbial ecology Normal microflora of man and animals. Rumen Brazil South America America Bacillus cereus Lysinibacillus sphaericus Bacillus thuringiensis Culicidae larvae and bacterial biodiversity Insecta Parasite Bacillaceae Biodiversity Infection Bacillales Larva Arthropoda Culicidae Bacteria Associated microflora Ectoparasite Invertebrata Diptera Microbial community
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Summary:	11,992 of Culex spp. and Aedes spp. larvae were collected, of which 10,321 larvae did not exhibit any apparent symptoms and 1671 exhibited some type of symptom that could not be attributed to a particular pathogen. [Display omitted] ► The aim was to investigate naturally occurring bacteria from Culicidae larvae. ► Identification was determined by cytomorphology, biochemical and physiological tests. ► One hundred and sixteen strains of bacteria were isolated and identified. ► Bacteria belonging to the genus Bacillus, Lysinibacillus and Brevibacillus. The aim of this study was to investigate the naturally occurring bacteria from Culicidae larvae found in São Paulo state between 2006 and 2008 from the Tiete Ecological Park in the municipality of Caraguatatuba. Bacterial strains were obtained after surface sterilization of larvae followed by thermal treatment and incubation in bacteriological media under laboratory conditions. Identification was determined using cytomorphology, biochemical and physiological tests. Strains were characterized by qualitative evaluation of biological activity against Culicidae larvae, comparing protein profiles obtained by electrophoresis of crystal protoxins, electrophoresis of Lysinibacillus sphaericus isoenzymes, detection of the L 2 fraction of Hemolytic BL enterotoxin and amplification of DNA using Multiplex-PCR to detect HBL, NHE, CytK, BceT and EntFM enterotoxins. The results obtained in this study demonstrated that the endosymbiotic bacterial diversity belonging to Bacillus and related genera associated with Culicidae larvae is limited to a number of species that does not vary throughout the seasons.
Bibliography:	http://dx.doi.org/10.1016/j.jip.2011.01.003
ISSN:	0022-2011 1096-0805
DOI:	10.1016/j.jip.2011.01.003