Defence‐Related Enzymes in Soybean Resistance to Target Spot

Defence‐Related Enzymes in Soybean Resistance to Target Spot

Target spot, caused by the fungus Corynespora cassiicola, has become a serious foliar disease in soybean production in the Brazilian Cerrado. Information in the literature regarding the biochemical defence responses of soybean to C. cassiicola infection is rare. Therefore, the objective of this stud...

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Bibliographic Details
Published in:Journal of phytopathology Vol. 163; no. 9; pp. 731 - 742
Main Authors: Fortunato, Alessandro A, Debona, Daniel, Bernardeli, Arthur M. A, Rodrigues, Fabrício A
Format: Journal Article
Language:English
Published: Berlin Parey 01-09-2015
Blackwell Publishing Ltd
Wiley Subscription Services, Inc
Subjects:
cerrado
chitinase
Corynespora cassiicola
crop production
cultivars
enzyme activity
foliar diseases
fungi
Glycine max
leaves
lignin
mechanisms of host defence
peroxidases
phenolic compounds
phenylalanine ammonia-lyase
soybeans
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Summary:Target spot, caused by the fungus Corynespora cassiicola, has become a serious foliar disease in soybean production in the Brazilian Cerrado. Information in the literature regarding the biochemical defence responses of soybean to C. cassiicola infection is rare. Therefore, the objective of this study was to determine the biochemical features associated with soybean resistance to target spot. The activities of chitinases (CHI), β‐1‐3‐glucanases (GLU), phenylalanine ammonia‐lyases (PAL), peroxidases (POX), polyphenol oxidases (PPO) and lipoxygenases (LOX), as well as the concentrations of total soluble phenolics (TSP) and lignin‐thioglycolic acid (LTGA) derivatives, were determined in soybean leaves from both a resistant (FUNDACEP 59) and a susceptible (TMG 132) cultivar. The target spot severity, number of lesions per cm² of leaflet and area under the disease progress curve were significantly lower for plants from cv. FUNDACEP 59 compared to plants from cv. TMG 132. The GLU, CHI, PAL, POX and PPO activities and the concentration of LTGA derivatives increased significantly, whereas LOX activity decreased significantly on the leaves infected by C. cassiicola. Inoculated plants from cv. FUNDACEP 59 showed a higher PPO activity and concentrations of TSP and LTGA derivatives at 4 and 6 days after inoculation compared to plants from cv. TMG 132. In conclusion, the results of this study demonstrated that the defence‐related enzyme activities increased upon C. cassiicola infection, regardless of the basal level of resistance of the cultivar studied. The increases in PPO activity and concentrations of TSP and LTGA derivatives, but lower LOX activity, at early stages of C. cassiicola infection were highly associated with soybean resistance to target spot.
Bibliography:http://dx.doi.org/10.1111/jph.12370
ArticleID:JPH12370
istex:4C56F4A891B04D352D8A0071A05B5F1D4A58EA92
FAPEMIG
ark:/67375/WNG-80BX2F9Z-P
ISSN:0931-1785
1439-0434
DOI:10.1111/jph.12370