Search Results - "Berkhout, Ben"

Dicer-independent processing of small RNA duplexes: mechanistic insights and applications by Herrera-Carrillo, Elena, Berkhout, Ben

“…MicroRNAs (miRNAs) play a pivotal role in the regulation of cellular gene expression via the conserved RNA interference (RNAi) mechanism. Biogenesis of the…”
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Tet-On Systems For Doxycycline-inducible Gene Expression by Das, Atze T, Tenenbaum, Liliane, Berkhout, Ben

“…The tetracycline-controlled Tet-Off and Tet-On gene expression systems are used to regulate the activity of genes in eukaryotic cells in diverse settings,…”
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Extinction of all infectious HIV in cell culture by the CRISPR-Cas12a system with only a single crRNA by Gao, Zongliang, Fan, Minghui, Das, Atze T, Herrera-Carrillo, Elena, Berkhout, Ben

“…The CRISPR-Cas9 system has been used for genome editing of various organisms. We reported inhibition of the human immunodeficiency virus (HIV) in cell culture…”
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CRISPR-Cas9 Can Inhibit HIV-1 Replication but NHEJ Repair Facilitates Virus Escape by Wang, Gang, Zhao, Na, Berkhout, Ben, Das, Atze T

“…Several recent studies demonstrated that the clustered regularly interspaced short palindromic repeats (CRISPR)-associated endonuclease Cas9 can be used for…”
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What do we measure when we measure cell-associated HIV RNA by Pasternak, Alexander O, Berkhout, Ben

“…Cell-associated (CA) HIV RNA has received much attention in recent years as a surrogate measure of the efficiency of HIV latency reversion and because it may…”
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Novel AgoshRNA molecules for silencing of the CCR5 co-receptor for HIV-1 infection by Herrera-Carrillo, Elena, Berkhout, Ben

“…Allogeneic transplantation of blood stem cells from a CCR5-Δ32 homozygous donor to an HIV-infected individual, the "Berlin patient", led to a cure. Since then…”
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CRISPR-Cas based antiviral strategies against HIV-1 by Wang, Gang, Zhao, Na, Berkhout, Ben, Das, Atze T.

“…•CRISPR-Cas9 can inhibit HIV replication.•CRISPR-Cas9 can permanently inactivate latent HIV.•CRISPR-dCas9 systems can reactivate latent HIV.•Novel CRISPR-Cas…”
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A CRISPR-Cas Cure for HIV/AIDS by Hussein, Mouraya, Molina, Mariano A, Berkhout, Ben, Herrera-Carrillo, Elena

“…Human immunodeficiency virus (HIV) infections and HIV-induced acquired immunodeficiency syndrome (AIDS) continue to represent a global health burden. There is…”
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The Splice of Life: Does RNA Processing Have a Role in HIV-1 Persistence? by Pasternak, Alexander O, Berkhout, Ben

“…Antiretroviral therapy (ART) suppresses HIV-1 replication but does not eradicate the virus. Persistence of HIV-1 latent reservoirs in ART-treated individuals…”
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Delineation of the Exact Transcription Termination Signal for Type 3 Polymerase III by Gao, Zongliang, Herrera-Carrillo, Elena, Berkhout, Ben

“…Type 3 Pol III promoters such as U6 are widely used for expression of small RNAs, including short hairpin RNA for RNAi applications and guide RNA in CRISPR…”
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RNA interference against viruses: strike and counterstrike by Berkhout, Ben, Haasnoot, Joost, Westerhout, Ellen M

“…RNA interference (RNAi) is a conserved sequence-specific, gene-silencing mechanism that is induced by double-stranded RNA. RNAi holds great promise as a novel…”
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Comparison of droplet digital PCR and seminested real-time PCR for quantification of cell-associated HIV-1 RNA by Kiselinova, Maja, Pasternak, Alexander O, De Spiegelaere, Ward, Vogelaers, Dirk, Berkhout, Ben, Vandekerckhove, Linos

“…Cell-associated (CA) HIV-1 RNA is considered a potential marker for assessment of viral reservoir dynamics and antiretroviral therapy (ART) response in…”
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Integration of SARS-CoV-2 RNA in infected human cells by retrotransposons: an unlikely hypothesis and old viral relationships by Grandi, Nicole, Tramontano, Enzo, Berkhout, Ben

“…Abstract Zhang et al. (Proc Natl Acad Sci 118:e2105968118, 2021) recently reported that SARS-CoV-2 RNA can be retrotranscribed and integrated into the DNA of…”
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CRISPR-Cas12b enables a highly efficient attack on HIV proviral DNA in T cell cultures by Fan, Minghui, Bao, Yuanling, Berkhout, Ben, Herrera-Carrillo, Elena

“…The novel endonuclease Cas12b was engineered for targeted genome editing in mammalian cells and is a promising tool for certain applications because of its…”
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CRISPR therapy towards an HIV cure by Herrera-Carrillo, Elena, Gao, Zongliang, Berkhout, Ben

“…Tools based on RNA interference (RNAi) and the recently developed clustered regularly short palindromic repeats (CRISPR) system enable the selective…”
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The Quest for Cellular Markers of HIV Reservoirs: Any Color You Like by Darcis, Gilles, Berkhout, Ben, Pasternak, Alexander O

“…Combination antiretroviral therapy (ART) suppresses human immunodeficiency virus (HIV) replication and improves immune function, but is unable to eradicate the…”
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The novel human coronaviruses NL63 and HKU1 by PYRC, Krzysztof, BERKHOUT, Ben, VAN DER HOEK, Lia

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IFI16 Targets the Transcription Factor Sp1 to Suppress HIV-1 Transcription and Latency Reactivation by Hotter, Dominik, Bosso, Matteo, Jønsson, Kasper L., Krapp, Christian, Stürzel, Christina M., Das, Atze, Littwitz-Salomon, Elisabeth, Berkhout, Ben, Russ, Alina, Wittmann, Sabine, Gramberg, Thomas, Zheng, Yue, Martins, Laura J., Planelles, Vicente, Jakobsen, Martin R., Hahn, Beatrice H., Dittmer, Ulf, Sauter, Daniel, Kirchhoff, Frank

“…The interferon γ-inducible protein 16 (IFI16) is known as immune sensor of retroviral DNA intermediates. We show that IFI16 restricts HIV-1 independently…”
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Impact of the biased nucleotide composition of viral RNA genomes on RNA structure and codon usage by van Hemert, Formijn, van der Kuyl, Antoinette C, Berkhout, Ben

“…We are interested in the influence of nucleotide composition on the fundamental characteristics of the virus RNA genome. Most RNA viruses have genomes with a…”
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A combinatorial CRISPR-Cas12a attack on HIV DNA by Fan, Minghui, Berkhout, Ben, Herrera-Carrillo, Elena

“…CRISPR-Cas12a is an alternative class 2 gene editing tool that may cause less off-target effects than the original Cas9 system. We have previously demonstrated…”
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