Search Results - "Bergquist, P. L"

Development of a Two-Color Fluorescence In Situ Hybridization Technique for Species-Level Identification of Human-Infectious Cryptosporidium spp by Alagappan, A, Bergquist, P.L, Ferrari, B.C

“…A two-color fluorescence in situ hybridization assay that allows for the simultaneous identification of Cryptosporidium parvum and C. hominis was developed…”
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Quantum dots as alternatives to organic fluorophores for Cryptosporidium detection using conventional flow cytometry and specific monoclonal antibodies: Lessons learned by Ferrari, B. C., Bergquist, P. L.

“…Background: Significant developments in biological applications are occurring through the incorporation of Quantum Dots (QDs) as biological labels. The…”
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Fluorescent reference strains of bacteria by chromosomal integration of a modified green fluorescent protein gene by Pinheiro, L. B, Gibbs, M. D, Vesey, G, Smith, J. J, Bergquist, P. L

“…Fluorescent reference strains of bacteria carrying a stable chromosomally integrated single copy of the gfp gene have been developed. A modified version of the…”
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Closed-tube DNA extraction using a thermostable proteinase is highly sensitive, capable of single parasite detection by Ferrari, B. C, Power, M. L, Bergquist, P. L

“…Current DNA extraction methods for parasites are labour-intensive and usually involve several steps, increasing the potential for cross-contamination. We…”
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Applying fluorescence based technology to the recovery and isolation of Cryptosporidium and Giardia from industrial wastewater streams by Ferrari, B.C., Stoner, K., Bergquist, P.L.

“…As increasing water shortages continue, water re-use is posing new challenges with treated wastewater becoming a significant source of non-potable water. Rapid…”
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Development of fluorescent in situ hybridisation for Cryptosporidium detection reveals zoonotic and anthroponotic transmission of sporadic cryptosporidiosis in Sydney by Alagappan, A., Tujula, N.A., Power, M., Ferguson, C.M., Bergquist, P.L., Ferrari, B.C.

“…Cryptosporidium, is the most common non-viral cause of diarrhea worldwide. Of the 5 described species that contribute to the majority of human infections, C…”
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Biolistic transformation of Trichoderma reesei using the Bio-Rad seven barrels Hepta Adaptor system by Te'o, V.S.J, Bergquist, P.L, Nevalainen, K.M.H

“…Effective biolistic transformation of intact conidia from the filamentous fungus Trichoderma reesei was achieved using the Bio-Rad Hepta Adaptor system with…”
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Rapid transformation of high cellulase‐producing mutant strains of Trichoderma reesei by microprojectile bombardment by Hazell, B.W., Te’O, V.S.J., Bradner, J.R., Bergquist, P.L., Nevalainen, K.M.H.

“…Intact conidia of three industrially relevant strains of Trichoderma reesei were effectively transformed by particle bombardment. Transformations were carried…”
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A novel thermostable multidomain 1,4-β-xylanase from 'Caldibacillus cellulovorans' and effect of its xylan-binding domain on enzyme activity by SUNNA, Anwar, GIBBS, Moreland D, BERGQUIST, Peter L

“…The nucleotide sequence of the complete xynA gene, encoding a novel multidomain xylanase XynA of 'Caldibacillus cellulovorans', was determined by…”
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Identification of novel beta-mannan- and beta-glucan-binding modules: evidence for a superfamily of carbohydrate-binding modules by Sunna, A, Gibbs, M D, Bergquist, P L

“…Many glycoside hydrolases, which degrade long-chain carbohydrate polymers, possess distinct catalytic modules and non-catalytic carbohydrate-binding modules…”
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Paenibacillus isolates possess diverse dextran-degrading enzymes by Finnegan, P.M, Brumbley, S.M, O'Shea, M.G, Nevalainen, K.M.H, Bergquist, P.L

“…Aims:  To isolate and identify dextran‐degrading organisms from sugar mill and compost samples, and to examine the diversity of the dextranolytic enzymes…”
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Description of Caldicellulosiruptor saccharolyticus gen. nov., sp. nov: an obligately anaerobic, extremely thermophilic, cellulolytic bacterium by Rainey, F A, Donnison, A M, Janssen, P H, Saul, D, Rodrigo, A, Bergquist, P L, Daniel, R M, Stackebrandt, E, Morgan, H W

“…A new obligately anaerobic, extremely thermophilic, cellulolytic bacterium is described. The strain designated Tp8T 6331 is differentiated from thermophilic…”
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Cloning, sequencing, and expression of a xylanase gene from the extreme thermophile Dictyoglomus thermophilum Rt46B.1 and activity of the enzyme on fiber-bound substrate by Gibbs, M.D. (University of Auckland, Auckland, New Zealand.), Reeves, R.A, Bergquist, P.L

“…A genomic library of the Dictyoglomus sp. strain Rt46B.1 was constructed in the phage vector lambda ZapII and screened for xylanase activity. A plaque…”
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Cloning of the xynB gene from Dictyoglomus thermophilum Rt46B.1 and action of the gene product on kraft pulp by MORRIS, D. D, GIBBS, M. D, CHIN, C. W. J, KOH, M.-H, WONG, K. K. Y, ALLISON, R. W, NELSON, P. J, BERGQUIST, P. L

“…A two-step PCR protocol was used to identify and sequence a family 11 xylanase gene from Dictyoglomus thermophilum Rt46B.1. Family 11 xylanase consensus…”
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Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilum for expression in the filamentous fungus Trichoderma reesei by Te'o, V.S.J, Cziferszky, A.E, Bergquist, P.L, Nevalainen, K.M.H

“…The catalytic domain of the xynB (xylanase) gene from the thermophilic bacterium Dictyoglomus thermophilum was reconstructed by PCR to match the codon…”
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Expression and processing of a major xylanase (XYN2) from the thermophilic fungus Humicola grisea var. thermoidea in Trichoderma reesei by De Faria, F.P., Te'O, V.S.J., Bergquist, P.L., Azevedo, M.O., Nevalainen, K.M.H.

“…Aims: To express a gene encoding a heterologous fungal xylanase in Trichoderma reesei. Methods and Results: Humicola grisea xylanase 2 (xyn2) cDNA was…”
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Correction of the beta-mannanase domain of the celC pseudogene from Caldocellulosiruptor saccharolyticus and activity of the gene product on kraft pulp by Morris, D.D. (University of Auckland, Auckland, New Zealand.), Reeves, R.A, Gibbs, M.D, Saul, D.J, Bergquist, P.L

“…The celA, manA, and celB genes from Caldocellulosiruptor saccharolyticus compose a cellulase-hemicellulase gene cluster and are arranged on a 12-kb C…”
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The beta-mannanase from "Caldocellum saccharolyticum" is part of a multidomain enzyme by GIBBS, M. D, SAUL, D. J, LÜTHI, E, BERGQUIST, P. L

“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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Heterologous protein expression in filamentous fungi by Nevalainen, K.M. Helena, Te'o, Valentino S.J., Bergquist, Peter L.

“…Filamentous fungi are commonly used in the fermentation industry for the large-scale production of proteins – mainly industrial enzymes. Recent advances in…”
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celA, another gene coding for a multidomain cellulase from the extreme thermophile Caldocellum saccharolyticum by Te'o, V S, Saul, D J, Bergquist, P L

“…Caldocellum saccharolyticum is an extremely thermophilic anaerobic bacterium capable of growth on cellulose and hemicellulose as sole carbon sources. Cellulase…”
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