Search Results - "Berger, Christopher L."

Acetylated Microtubules Are Preferentially Bundled Leading to Enhanced Kinesin-1 Motility by Balabanian, Linda, Berger, Christopher L., Hendricks, Adam G.

“…The motor proteins kinesin and dynein transport organelles, mRNA, proteins, and signaling molecules along the microtubule cytoskeleton. In addition to serving…”
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Tau directs intracellular trafficking by regulating the forces exerted by kinesin and dynein teams by Chaudhary, Abdullah R., Berger, Florian, Berger, Christopher L., Hendricks, Adam G.

“…Organelles, proteins, and mRNA are transported bidirectionally along microtubules by plus‐end directed kinesin and minus‐end directed dynein motors…”
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Polyglutamylation of tubulin's C-terminal tail controls pausing and motility of kinesin-3 family member KIF1A by Lessard, Dominique V., Zinder, Oraya J., Hotta, Takashi, Verhey, Kristen J., Ohi, Ryoma, Berger, Christopher L.

“…The kinesin-3 family member KIF1A plays a critical role in site-specific neuronal cargo delivery during axonal transport. KIF1A cargo is mislocalized in many…”
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Optogenetic control of kinesin-1, -2, -3 and dynein reveals their specific roles in vesicular transport by Nagpal, Sahil, Swaminathan, Karthikeyan, Beaudet, Daniel, Verdier, Maud, Wang, Samuel, Berger, Christopher L., Berger, Florian, Hendricks, Adam G.

“…Each cargo in a cell employs a unique set of motor proteins for its transport. To dissect the roles of each type of motor, we developed optogenetic inhibitors…”
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The Nucleotide-binding State of Microtubules Modulates Kinesin Processivity and the Ability of Tau to Inhibit Kinesin-mediated Transport by McVicker, Derrick P., Chrin, Lynn R., Berger, Christopher L.

“…The ability of Tau to act as a potent inhibitor of kinesin's processive run length in vitro suggests that it may actively participate in the regulation of…”
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Breaking the Millisecond Barrier: Single Molecule Motors Wobble to Find their Next Binding Sites by Berger, Christopher L.

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Single-Molecule Motility: Statistical Analysis and the Effects of Track Length on Quantification of Processive Motion by Thompson, Andrew R., Hoeprich, Gregory J., Berger, Christopher L.

“…In vitro, single-molecule motility assays allow for the direct characterization of molecular motor properties including stepping velocity and characteristic…”
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Tau, microtubule dynamics, and axonal transport: New paradigms for neurodegenerative disease by Cario, Alisa, Berger, Christopher L.

“…The etiology of Tauopathies, a diverse class of neurodegenerative diseases associated with the Microtubule Associated Protein (MAP) Tau, is usually described…”
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Kinesin’s Neck-Linker Determines its Ability to Navigate Obstacles on the Microtubule Surface by Hoeprich, Gregory J., Thompson, Andrew R., McVicker, Derrick P., Hancock, William O., Berger, Christopher L.

“…The neck-linker is a structurally conserved region among most members of the kinesin superfamily of molecular motor proteins that is critical for kinesin’s…”
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The types and numbers of kinesins and dyneins transporting endocytic cargoes modulate their motility and response to tau by Beaudet, Daniel, Berger, Christopher L., Hendricks, Adam G.

“…Organelles and vesicular cargoes are transported by teams of kinesin and dynein motors along microtubules. We isolated endocytic organelles from cells at…”
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Modification of the neck-linker of KIF18A alters Microtubule subpopulation preference by Queen, Katelyn A, Cario, Alisa, Berger, Christopher L, Stumpff, Jason

“…Kinesins support many diverse cellular processes, including facilitating cell division through mechanical regulation of the mitotic spindle. However, how…”
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Tau acts as a selective barrier along microtubules to regulate multi-motor cargo transport by Beaudet, Daniel, Berger, Christopher L., Hendricks, Adam G.

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The N-terminal disease–associated R5L Tau mutation increases microtubule shrinkage rate due to disruption of microtubule-bound Tau patches by Cario, Alisa, Wickramasinghe, Sanjula P., Rhoades, Elizabeth, Berger, Christopher L.

“…Regulation of the neuronal microtubule cytoskeleton is achieved through the coordination of microtubule-associated proteins (MAPs). MAP-Tau, the most abundant…”
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The pathogenic R5L mutation disrupts formation of Tau complexes on the microtubule by altering local N-terminal structure by Cario, Alisa, Savastano, Adriana, Wood, Neil B, Liu, Zhu, Previs, Michael J, Hendricks, Adam G, Zweckstetter, Markus, Berger, Christopher L

“…The microtubule-associated protein (MAP) Tau is an intrinsically disordered protein (IDP) primarily expressed in axons, where it functions to regulate…”
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Phosphoregulation of Tau modulates inhibition of kinesin-1 motility by Stern, Jamie L, Lessard, Dominique V, Hoeprich, Gregory J, Morfini, Gerardo A, Berger, Christopher L

“…Microtubule-based axonal transport is tightly regulated by numerous pathways, ensuring appropriate delivery of specific organelle cargoes to selected…”
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Tau differentially regulates the transport of early endosomes and lysosomes by Balabanian, Linda, Lessard, Dominique V, Swaminathan, Karthikeyan, Yaninska, Pamela, Sébastien, Muriel, Wang, Samuel, Stevens, Piper W, Wiseman, Paul W, Berger, Christopher L, Hendricks, Adam G

“…Microtubule-associated proteins (MAPs) modulate the motility of kinesin and dynein along microtubules to control the transport of vesicles and organelles. The…”
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The axonal transport motor kinesin‐2 navigates microtubule obstacles via protofilament switching by Hoeprich, Gregory J., Mickolajczyk, Keith J., Nelson, Shane R., Hancock, William O., Berger, Christopher L.

“…Axonal transport involves kinesin motors trafficking cargo along microtubules that are rich in microtubule‐associated proteins (MAPs). Much attention has…”
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Kinetics of Presynaptic Filament Assembly in the Presence of Single-Stranded DNA Binding Protein and Recombination Mediator Protein by Liu, Jie, Berger, Christopher L, Morrical, Scott W

“…Enzymes of the RecA/Rad51 family catalyze DNA strand exchange reactions that are important for homologous recombination and for the accurate repair of DNA…”
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Fluorescence anisotropy and resonance energy transfer: powerful tools for measuring real time protein dynamics in a physiological environment by Yengo, Christopher M, Berger, Christopher L

“…Fluorescence spectroscopy/microscopy is a versatile method for examining protein dynamics in vitro and in vivo that can be combined with other techniques to…”
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Actin-induced Closure of the Actin-binding Cleft of Smooth Muscle Myosin by Yengo, Christopher M., De La Cruz, Enrique M., Chrin, Lynn R., Gaffney, Donald P., Berger, Christopher L.

“…The putative actin-binding interface of myosin is separated by a large cleft that extends into the base of the nucleotide binding pocket, suggesting that it…”
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