Tendon healing induced by chemically modified mRNAs

Tendon healing induced by chemically modified mRNAs

Tendon disorders are frequent both in human and veterinary medicine with high re-injury rates and unsatisfactory therapeutic treatments. Application of naked, chemically-modified mRNA (cmRNA), encoding for therapeutic proteins, is an innovative approach to address tendon healing. In the current stud...

Full description

Saved in:
Bibliographic Details
Published in:European cells & materials Vol. 33; pp. 294 - 307
Main Authors: Groth, K, Berezhanskyy, T, Aneja, M K, Geiger, J, Schweizer, M, Maucksch, L, Pasewald, T, Brill, T, Tigani, B, Weber, E, Rudolph, C, Hasenpusch, G
Format: Journal Article
Language:English
Published: Switzerland Forum Multimedia Publishing LLC 24-05-2017
Subjects:
Animals
Calcaneus - injuries
Calcaneus - pathology
Female
gene therapy
Kinetics
mRNA
Rats, Sprague-Dawley
regenerative medicine
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Messenger - therapeutic use
Sheep
Solvents
tendinopathy
Tendon Injuries - pathology
Tendon Injuries - therapy
tendon rupture
Tendons - pathology
Transfection
Wound Healing
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tendon disorders are frequent both in human and veterinary medicine with high re-injury rates and unsatisfactory therapeutic treatments. Application of naked, chemically-modified mRNA (cmRNA), encoding for therapeutic proteins, is an innovative approach to address tendon healing. In the current study, we demonstrated that injection of naked cmRNA, diluted in a glucose-containing solution, into tendons resulted in high protein expression in healthy and experimentally-injured tendons. Injection of bone morphogenetic protein 7 (BMP-7)-encoding cmRNA resulted in a significantly higher expression of BMP-7 protein and reduced formation of collagen type III, compared to vehicle control. Moreover, in a large animal model, reporter protein expression was detectable not only in healthy, but also in experimentally-injured, severely inflamed tendons. Summarising, these results demonstrated the potential of cmRNAs encoding for therapeutic proteins as a new class of drugs for the treatment of tendon disorders.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1473-2262
DOI:10.22203/eCM.v033a22