Search Results - "Benson, David E."

Role of global public sector research in discovering new drugs and vaccines by Stevens, Ashley J., Benson, David E., Dodson, Sara E., Jensen, Jonathan J., Rohrbaugh, Mark L.

“…Analysis of international public-sector contributions to Food and Drug Administration (FDA)-approved drugs and vaccines allows for a more thorough examination…”
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The Catalytic Pathway of Cytochrome P450cam at Atomic Resolution by Schlichting, Ilme, Berendzen, Joel, Chu, Kelvin, Stock, Ann M., Maves, Shelley A., Benson, David E., Sweet, Robert M., Ringe, Dagmar, Petsko, Gregory A., Sligar, Stephen G.

“…Members of the cytochrome P450 superfamily catalyze the addition of molecular oxygen to nonactivated hydrocarbons at physiological temperature-a reaction that…”
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Simulating Metal-Imidazole Complexes by Li, Zhen, Bhowmik, Subhamoy, Sagresti, Luca, Brancato, Giuseppe, Smith, Madelyn, Benson, David E., Li, Pengfei, Merz, Kenneth M.

“…One commonly observed binding motif in metalloproteins involves the interaction between a metal ion and histidine’s imidazole side chains. Although previous…”
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Rational Design of Nascent Metalloenzymes by Benson, David E., Wisz, Michael S., Hellinga, Homme W.

“…Understanding the early genesis of new enzymatic functions is one of the challenges in protein design, mechanistic enzymology, and molecular evolution. We have…”
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A Metalloantibody That Irreversibly Binds a Protein Antigen by Trisler, Kirk, Looger, Loren L., Sharma, Vikram, Baker, Martin, Benson, David E., Trauger, Sunia, Schultz, Peter G., Smider, Vaughn V.

“…Antibody affinity is critically important in therapeutic applications, as well as steady state diagnostic assays. Picomolar affinity antibodies, approaching…”
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Analysis of allosteric signal transduction mechanisms in an engineered fluorescent maltose biosensor by Dattelbaum, Jonathan D., Looger, Loren L., Benson, David E., Sali, Kevin M., Thompson, Richard B., Hellinga, Homme W.

“…We previously reported the construction of a family of reagentless fluorescent biosensor proteins by the structure‐based design of conjugation sites for a…”
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Metallothioneins Initiate Semiconducting Nanoparticle Cellular Toxicity by Aryal, Baikuntha P., Neupane, Kosh P., Sandros, Marinella G., Benson, David E.

“…The toxicity of CdSe nanoparticles is mediated by metallothioneins (MTs), which are ubiquitous and cysteine‐rich. Surface Cd2+ ions are depleted from…”
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A Modular Nanoparticle-Based System for Reagentless Small Molecule Biosensing by Sandros, Marinella G, Gao, De, Benson, David E

“…Metalloprotein tethered CdSe nanoparticles have been generated to provide selective and reagentless maltose biosensing. As opposed to cell or protein detection…”
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Protein Design Provides Lead(II) Ion Biosensors for Imaging Molecular Fluxes around Red Blood Cells by Shete, Vivekanand S, Benson, David E

“…Metalloprotein design and semiconductor nanoparticles have been combined to generate a reagent for selective fluorescence imaging of Pb2+ ions in the presence…”
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Protein oxidation involved in Cys-Tyr post-translational modification by Hromada, Susan E., Hilbrands, Adam M., Wolf, Elysa M., Ross, Jackson L., Hegg, Taylor R., Roth, Andrew G., Hollowell, Matthew T., Anderson, Carolyn E., Benson, David E.

“…Some post-translationally modified tyrosines can perform reversible redox chemistry similar to metal cofactors. The most studied of these tyrosine…”
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Unimolecular, Soluble Semiconductor Nanoparticle-Based Biosensors for Thrombin Using Charge/Electron Transfer by Swain, Marla D, Octain, Jashain, Benson, David E

“…Duplex DNA was attached to semiconductor nanoparticles providing selective detection of thrombin. Using the method reported here, semiconductor nanoparticles…”
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Wide Dynamic Range Sensing with Single Quantum Dot Biosensors by Opperwall, Stacey R, Divakaran, Anand, Porter, Elizabeth G, Christians, Jeffrey A, DenHartigh, Andrew J, Benson, David E

“…Single-particle analysis of biosensors that use charge transfer as the means for analyte-dependent signaling with semiconductor nanoparticles, or quantum dots,…”
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Electron Donor Solvent Effects Provide Biosensing with Quantum Dots by Aryal, Baikuntha P, Benson, David E

“…A palmitate biosensor that uses the emission intensity of a semiconducting nanoparticle to report palmitate concentration is presented. This method uses…”
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The development of new biotechnologies using metalloprotein design by Benson, David E, Wisz, Michael S, Hellinga, Homme W

“…A variety of methodologies are under development to alter the behavior of existing metal centers or create entirely new sites within a protein framework in…”
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Selective, reversible, reagentless maltose biosensing with core-shell semiconducting nanoparticles by Sandros, Marinella G, Shete, Vivekanand, Benson, David E

“…Reagentless and reversible maltose biosensors are demonstrated using ZnS coated CdSe (CdSe@ZnS) nanoparticle emission intensities. This method is based on…”
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Identifying proteins that can form tyrosine-cysteine crosslinks by Martinie, Ryan J, Godakumbura, Pahan I, Porter, Elizabeth G, Divakaran, Anand, Burkhart, Brandon J, Wertz, John T, Benson, David E

“…Protein cofactors represent a unique class of redox active posttranslational protein modifications formed in or by metalloproteins. Once formed, protein…”
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General, high-affinity approach for the synthesis of fluorophore appended protein nanoparticle assemblies by Sandros, Marinella G, Gao, De, Gokdemir, Cagil, Benson, David E

“…Metallothionein fusion proteins allow for site-specific, orthogonal functionalization of proteins to a variety of nanoparticles…”
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Design of Bioelectronic Interfaces by Exploiting Hinge-Bending Motions in Proteins by Benson, David E., Conrad, David W., de Lorimier, Robert M., Trammell, Scott A., Hellinga, Homme W.

“…We report a flexible strategy for transducing ligand-binding events into electrochemical responses for a wide variety of proteins. The method exploits…”
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Polyhistidine Fusion Proteins Can Nucleate the Growth of CdSe Nanoparticles by Aryal, Baikuntha P, Benson, David E

“…A (His-Asn)6 domain fused to fatty acid binding protein provides the low-temperature assembly of CdSe nanoparticles starting with common inorganic salt…”
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Converting a Maltose Receptor into a Nascent Binuclear Copper Oxygenase by Computational Design by Benson, David E, Haddy, Alice E, Hellinga, Homme W

“…Computational protein design methods were used to identify mutations that are predicted to introduce a binuclear copper center coordinated by six histidines,…”
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