Micro‐Topographies Induce Epigenetic Reprogramming and Quiescence in Human Mesenchymal Stem Cells
Biomaterials can control cell and nuclear morphology. Since the shape of the nucleus influences chromatin architecture, gene expression and cell identity, surface topography can control cell phenotype. This study provides fundamental insights into how surface topography influences nuclear morphology...
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| Published in: | Advanced science Vol. 10; no. 1; pp. e2203880 - n/a |
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| Main Authors: | , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Germany
John Wiley & Sons, Inc
01-01-2023
John Wiley and Sons Inc Wiley |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Biomaterials can control cell and nuclear morphology. Since the shape of the nucleus influences chromatin architecture, gene expression and cell identity, surface topography can control cell phenotype. This study provides fundamental insights into how surface topography influences nuclear morphology, histone modifications, and expression of histone‐associated proteins through advanced histone mass spectrometry and microarray analysis. The authors find that nuclear confinement is associated with a loss of histone acetylation and nucleoli abundance, while pathway analysis reveals a substantial reduction in gene expression associated with chromosome organization. In light of previous observations where the authors found a decrease in proliferation and metabolism induced by micro‐topographies, they connect these findings with a quiescent phenotype in mesenchymal stem cells, as further shown by a reduction of ribosomal proteins and the maintenance of multipotency on micro‐topographies after long‐term culture conditions. Also, this influence of micro‐topographies on nuclear morphology and proliferation is reversible, as shown by a return of proliferation when re‐cultured on a flat surface. The findings provide novel insights into how biophysical signaling influences the epigenetic landscape and subsequent cellular phenotype.
Biomaterials can profoundly affect the size and shape of the nucleus, yet the phenotypical consequences of these alterations are still largely unknown. In this work, the effect of nuclear confinement on mesenchymal stem cell behavior is elucidated through histone mass‐spectrometry and microarray datasets. Here, a reduction of ribosomal and nucleoli proteins and histone acetylation is connected with a quiescent phenotype. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 2198-3844 2198-3844 |
| DOI: | 10.1002/advs.202203880 |