Search Results - "Benesch, Justin L.P"

Collision Cross Sections for Structural Proteomics by Marklund, Erik G., Degiacomi, Matteo T., Robinson, Carol V., Baldwin, Andrew J., Benesch, Justin L.P.

“…Ion mobility mass spectrometry (IM-MS) allows the structural interrogation of biomolecules by reporting their collision cross sections (CCSs). The major…”
Get full text

Small Heat Shock Protein Activity Is Regulated by Variable Oligomeric Substructure by Benesch, Justin L.P., Ayoub, Marina, Robinson, Carol V., Aquilina, J. Andrew

“…The α-crystallins are members of the small heat shock protein family of molecular chaperones that have evolved to minimize intracellular protein aggregation;…”
Get full text

Defining the Structural Basis of Human Plasminogen Binding by Streptococcal Surface Enolase by Cork, Amanda J., Jergic, Slobodan, Hammerschmidt, Sven, Kobe, Bostjan, Pancholi, Vijay, Benesch, Justin L.P., Robinson, Carol V., Dixon, Nicholas E., Aquilina, J. Andrew, Walker, Mark J.

“…The flesh-eating bacterium group A Streptococcus (GAS) binds and activates human plasminogen, promoting invasive disease. Streptococcal surface enolase (SEN),…”
Get full text

Quantifying Protein–Protein Interactions by Molecular Counting with Mass Photometry by Soltermann, Fabian, Foley, Eric D. B., Pagnoni, Veronica, Galpin, Martin, Benesch, Justin L. P., Kukura, Philipp, Struwe, Weston B.

“…Interactions between biomolecules control the processes of life in health and their malfunction in disease, making their characterization and quantification…”
Get full text

Origin of complexity in haemoglobin evolution by Pillai, Arvind S., Chandler, Shane A., Liu, Yang, Signore, Anthony V., Cortez-Romero, Carlos R., Benesch, Justin L. P., Laganowsky, Arthur, Storz, Jay F., Hochberg, Georg K. A., Thornton, Joseph W.

“…Most proteins associate into multimeric complexes with specific architectures 1 , 2 , which often have functional properties such as cooperative ligand binding…”
Get full text

An ultra-stable gold-coordinated protein cage displaying reversible assembly by Malay, Ali D., Miyazaki, Naoyuki, Biela, Artur, Chakraborti, Soumyananda, Majsterkiewicz, Karolina, Stupka, Izabela, Kaplan, Craig S., Kowalczyk, Agnieszka, Piette, Bernard M. A. G., Hochberg, Georg K. A., Wu, Di, Wrobel, Tomasz P., Fineberg, Adam, Kushwah, Manish S., Kelemen, Mitja, Vavpetič, Primož, Pelicon, Primož, Kukura, Philipp, Benesch, Justin L. P., Iwasaki, Kenji, Heddle, Jonathan G.

“…Symmetrical protein cages have evolved to fulfil diverse roles in nature, including compartmentalization and cargo delivery 1 , and have inspired synthetic…”
Get full text

Ion mobility-mass spectrometry analysis of large protein complexes by Ruotolo, Brandon T, Robinson, Carol V, Benesch, Justin L P, Sandercock, Alan M, Hyung, Suk-Joon

“…Here we describe a detailed protocol for both data collection and interpretation with respect to ion mobility–mass spectrometry analysis of large protein…”
Get full text

The Role of the Detergent Micelle in Preserving the Structure of Membrane Proteins in the Gas Phase by Reading, Eamonn, Liko, Idlir, Allison, Timothy M., Benesch, Justin L. P., Laganowsky, Arthur, Robinson, Carol V.

“…Despite the growing importance of the mass spectrometry of membrane proteins, it is not known how their transfer from solution into vacuum affects their…”
Get full text

Optimal Synthetic Glycosylation of a Therapeutic Antibody by Parsons, Thomas B., Struwe, Weston B., Gault, Joseph, Yamamoto, Keisuke, Taylor, Thomas A., Raj, Ritu, Wals, Kim, Mohammed, Shabaz, Robinson, Carol V., Benesch, Justin L. P., Davis, Benjamin G.

“…Glycosylation patterns in antibodies critically determine biological and physical properties but their precise control is a significant challenge in biology…”
Get full text

Detergent-free mass spectrometry of membrane protein complexes by Hopper, Jonathan T S, Yu, Yvonne Ting-Chun, Li, Dianfan, Raymond, Alison, Bostock, Mark, Liko, Idlir, Mikhailov, Victor, Laganowsky, Arthur, Benesch, Justin L P, Caffrey, Martin, Nietlispach, Daniel, Robinson, Carol V

“…Amphipols, bicelles and nanodiscs are used to study intact membrane protein complexes by mass spectrometry, with better preservation of oligomeric complexes…”
Get full text

Accommodating Protein Dynamics in the Modeling of Chemical Crosslinks by Degiacomi, Matteo T., Schmidt, Carla, Baldwin, Andrew J., Benesch, Justin L.P.

“…Chemical crosslinking can identify the neighborhood relationships between specific amino-acid residues in proteins. The interpretation of crosslinking data is…”
Get full text

Real-Time Intrinsic Fluorescence Visualization and Sizing of Proteins and Protein Complexes in Microfluidic Devices by Challa, Pavan Kumar, Peter, Quentin, Wright, Maya A, Zhang, Yuewen, Saar, Kadi L, Carozza, Jacqueline A, Benesch, Justin L. P, Knowles, Tuomas P. J

“…Optical detection has become a convenient and scalable approach to read out information from microfluidic systems. For the study of many key biomolecules,…”
Get full text

αB-Crystallin Polydispersity Is a Consequence of Unbiased Quaternary Dynamics by Baldwin, Andrew J., Lioe, Hadi, Robinson, Carol V., Kay, Lewis E., Benesch, Justin L.P.

“…The inherent heterogeneity of many protein assemblies complicates characterization of their structure and dynamics, as most biophysical techniques require…”
Get full text

Quaternary dynamics and plasticity underlie small heat shock protein chaperone function by Stengel, Florian, Baldwin, Andrew J, Painter, Alexander J, Jaya, Nomalie, Basha, Eman, Kay, Lewis E, Vierling, Elizabeth, Robinson, Carol V, Benesch, Justin L.P

“…Small Heat Shock Proteins (sHSPs) are a diverse family of molecular chaperones that prevent protein aggregation by binding clients destabilized during cellular…”
Get full text

Terminal Regions Confer Plasticity to the Tetrameric Assembly of Human HspB2 and HspB3 by Clark, Alice R., Vree Egberts, Wilma, Kondrat, Frances D.L., Hilton, Gillian R., Ray, Nicholas J., Cole, Ambrose R., Carver, John A., Benesch, Justin L.P., Keep, Nicholas H., Boelens, Wilbert C., Slingsby, Christine

“…Heterogeneity in small heat shock proteins (sHsps) spans multiple spatiotemporal regimes—from fast fluctuations of part of the protein, to conformational…”
Get full text

Trajectory Taken by Dimeric Cu/Zn Superoxide Dismutase through the Protein Unfolding and Dissociation Landscape Is Modulated by Salt Bridge Formation by McAlary, Luke, Harrison, Julian A, Aquilina, J. Andrew, Fitzgerald, Steven P, Kelso, Celine, Benesch, Justin L.P, Yerbury, Justin J

“…Native mass spectrometry (MS) is a powerful means for studying macromolecular protein assemblies, including accessing activated states. However, much remains…”
Get full text

Quaternary Dynamics of αB-Crystallin as a Direct Consequence of Localised Tertiary Fluctuations in the C-Terminus by Baldwin, Andrew J., Hilton, Gillian R., Lioe, Hadi, Bagnéris, Claire, Benesch, Justin L.P., Kay, Lewis E.

“…The majority of proteins exist in vivo within macromolecular assemblies whose functions are dependent on dynamical processes spanning a wide range of time…”
Get full text

The Polydispersity of αB-Crystallin Is Rationalized by an Interconverting Polyhedral Architecture by Baldwin, Andrew J., Lioe, Hadi, Hilton, Gillian R., Baker, Lindsay A., Rubinstein, John L., Kay, Lewis E., Benesch, Justin L.P.

“…We report structural models for the most abundant oligomers populated by the polydisperse molecular chaperone αB-crystallin. Subunit connectivity is determined…”
Get full text

Coupling Microdroplet Microreactors with Mass Spectrometry: Reading the Contents of Single Droplets Online by Fidalgo, Luis M, Whyte, Graeme, Ruotolo, Brandon T, Benesch, Justin L.P, Stengel, Florian, Abell, Chris, Robinson, Carol V, Huck, Wilhelm T.S

“…Fully integrated: Mass spectrometry has been integrated into a detection scheme for microdroplets that are created within microfluidic channels (see picture,…”
Get full text

Phosphomimics Destabilize Hsp27 Oligomeric Assemblies and Enhance Chaperone Activity by Jovcevski, Blagojce, Kelly, Megan A., Rote, Anthea P., Berg, Tracey, Gastall, Heidi Y., Benesch, Justin L.P., Aquilina, J. Andrew, Ecroyd, Heath

“…Serine phosphorylation of the mammalian small heat-shock protein Hsp27 at residues 15, 78, and 82 is thought to regulate its structure and chaperone function;…”
Get full text