Ancylobacter radicis sp. nov., a novel aerobic methylotrophic bacteria associated with plants

The two novel bacterial strains, designated as VT T and ML, were isolated from roots of cinquefoil ( Potentilla sp.) and leaves of meadow-grass ( Poa sp.) on the flooded bank of lake, respectively. These isolates were Gram-negative, non-spore-forming, non-motile, rod-shaped cells, utilized methanol,...

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Bibliographic Details
Published in:	Antonie van Leeuwenhoek Vol. 116; no. 9; pp. 855 - 866
Main Authors:	Agafonova, Nadezhda V., Belova, Alina A., Kaparullina, Elena N., Tarlachkov, Sergey V., Kopitsyn, Dmitry S., Machulin, Andrey V., Doronina, Nina V.
Format:	Journal Article
Language:	English
Published:	Cham Springer International Publishing 01-09-2023 Springer Nature B.V
Subjects:	Amino acids Bacteria Biomedical and Life Sciences Biosynthesis Carbon sources Deoxyribonucleic acid DNA Energy sources Exopolysaccharides Fatty acids Gene sequencing Genes Genomes Hormones Hybridization Life Sciences Medical Microbiology Metabolism Methylamine Methylotrophic bacteria Microbiology Natural products New species Nucleotides Original Paper Phosphates Phosphorus metabolism Phylogenetics Phylogeny Plant metabolism Plant Sciences Polyhydroxybutyrate Polyhydroxybutyric acid rRNA 16S Siderophores Soil Science & Conservation Strains (organisms) Ribulose-bisphosphate pathway sp. nov Methylotrophic bacteria Ancylobacter radicis sp. nov
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Summary:	The two novel bacterial strains, designated as VT T and ML, were isolated from roots of cinquefoil ( Potentilla sp.) and leaves of meadow-grass ( Poa sp.) on the flooded bank of lake, respectively. These isolates were Gram-negative, non-spore-forming, non-motile, rod-shaped cells, utilized methanol, methylamine, and polycarbon compounds as carbon and energy sources. In the whole-cell fatty acid pattern of strains prevailed C 18:1 ω 7 c and C 19:0cyc . Based on the phylogenetic analysis of 16S rRNA gene sequences, strains VT T and ML were closely related to the representatives of the genus Ancylobacter (98.3–98.5%). The assembled genome of strain VT T has a total length of 4.22 Mbp, and a G + C content is 67.3%. The average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA–DNA hybridization (dDDH) values between strain VT T and closely related type strains of genus Ancylobacter were 78.0–80.6%, 73.8–78.3% and 22.1–24.0%, respectively, that clearly lower than proposed thresholds for species. On the basis of the phylogenetic, phenotypic, and chemotaxonomic analysis, isolates VT T and ML represent a novel species of the genus Ancylobacter , for which the name Ancylobacter radicis sp. nov. is proposed. The type strain is VT T (= VKM B-3255 T = CCUG 72400 T ). In addition, novel strains were able to dissolve insoluble phosphates, to produce siderophores and plant hormones (auxin biosynthesis). According to genome analysis genes involved in the biosynthesis of siderophores, polyhydroxybutyrate, exopolysaccharides and phosphorus metabolism, as well as the genes involved in the assimilation of C 1 -compounds (natural products of plant metabolism) were found in the genome of type strain VT T .
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0003-6072 1572-9699
DOI:	10.1007/s10482-023-01850-z