Рroblems of PCR diagnostics OF COVID-19

Рroblems of PCR diagnostics OF COVID-19

To date, an adequate and timely assessment of the number of cases is the basis of effective measures aimed at preventing the spread of COVID-19 infection. Real-time reverse transcription polymerase chain reaction (RT-PCR) remains the gold standard for confirming COVID-19. The purpose of the work: to...

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Bibliographic Details
Published in:Zhurnal infektologii Vol. 14; no. 3; pp. 55 - 60
Main Authors: Grigorieva, T. D., Belopolskaya, M. А.
Format: Journal Article
Language:English
Russian
Published: Journal Infectology 22-10-2022
Subjects:
covid-19
pcr
sars-cov-2
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Summary:To date, an adequate and timely assessment of the number of cases is the basis of effective measures aimed at preventing the spread of COVID-19 infection. Real-time reverse transcription polymerase chain reaction (RT-PCR) remains the gold standard for confirming COVID-19. The purpose of the work: to analyze the experience of the city virological center of the S.P. Botkin Clinical Infectious Diseases Hospital (Botkin Hospital) for the examination for the presence of SARS-CoV-2 coronavirus by PCR in the period from 2020 to 2022. Materials and methods . The systematization of PCR studies on COVID-19 for the period 2020-2022 was carried out. A total of 221,901 people were examined, positive results were obtained in 55,372 (24.95%). Among the contingents of the examined patients, patients who underwent inpatient treatment at the Botkin Hospital. Conclusions. This study analyzed the possible causes of false-positive and false-negative PCR results. The correlation of the number of positive results with the dynamics of detection of new cases of COVID-19 in St. Petersburg during the 2020-2022 pandemic is shown. It has been established that the proportion of patients examined more than 3 times during the period of hospitalization remains significant. This fact requires the closest attention, given the high cost and laboriousness of PCR studies.
ISSN:2072-6732
DOI:10.22625/2072-6732-2022-14-3-55-60