Inactivation of the Sinorhizobium fredii HH103 rhcJ gene abolishes nodulation outer proteins (Nops) secretion and decreases the symbiotic capacity with soybean

It has been postulated that nodulation outer proteins (Nops) avoid effective nodulation of Sinorhizobium fredii USDA257 to nodulate with American soybeans. S. fredii HH103 naturally nodulates with both Asiatic (non-commercial) and American (commercial) soybeans. Inactivation of the S. fredii HH103 g...

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Published in:	International microbiology Vol. 9; no. 2; pp. 125 - 133
Main Authors:	de Lyra, Maria do C C P, Lopez-Baena, F Javier, Madinabeitia, Nuria, Vinardell, Jośe María, Espuny, Maria del Rosario, Cubo, María Teresa, Belloguin, Ramón Andrés, Ruiz-Sainz, Jośe Enrique, Ollero, Francisco Javier
Format:	Journal Article
Language:	English
Published:	Switzerland Sociedad Española de Microbiología 01-06-2006
Subjects:	Amino Acid Sequence Bacterial Proteins - genetics Bacterial Proteins - metabolism Gene Silencing Genes, Bacterial Glycine max - growth & development Glycine max - microbiology Molecular Sequence Data Mutation Sinorhizobium fredii Sinorhizobium fredii - genetics Sinorhizobium fredii - metabolism Sinorhizobium fredii - physiology Sinorhizobium fredii HH103 · gene rhcJ · type III secretion system · nodulation outer proteins (Nops) · soybean Symbiosis
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Summary:	It has been postulated that nodulation outer proteins (Nops) avoid effective nodulation of Sinorhizobium fredii USDA257 to nodulate with American soybeans. S. fredii HH103 naturally nodulates with both Asiatic (non-commercial) and American (commercial) soybeans. Inactivation of the S. fredii HH103 gene rhcJ, which belongs to the tts (type III secretion) cluster, abolished Nop secretion and decreased its symbiotic capacity with the two varieties of soybeans. S. fredii strains HH103 and USDA257, that only nodulates with Asian soybeans, showed different SDS-PAGE Nop profiles, indicating that these strains secrete different sets of Nops. In coinoculation experiments, the presence of strain USDA257 provoked a clear reduction of the nodulation ability of strain HH103 with the American soybean cultivar Williams. These results suggest that S. fredii Nops can act as either detrimental or beneficial symbiotic factors in a strain-cultivar-dependent manner. Differences in the flavonoid-mediated expression of rhcJ with respect to nodA were also detected. In addition, one of the Nops secreted by strain HH103 was identified as NopA.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1139-6709 1618-1905