CXCR4 and CXCL12 immunoreactivities differentiate primary non-small-cell lung cancer with or without brain metastases

Synchronous or metachronous brain metastases (BMs) occur in about 33% of patients affected by non-small-cell lung cancer (NSCLC). To date, no reliable biological marker is able to identify patients who will develop BMs. In the present study, using a quantitative double-labeling immunofluorescence an...

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Bibliographic Details
Published in:	Cancer biomarkers : section A of Disease markers Vol. 10; no. 2; p. 79
Main Authors:	Paratore, Sabrina, Banna, Giuseppe Luigi, D'Arrigo, Maria, Saita, Salvatore, Iemmolo, Rosario, Lucenti, Letizia, Bellia, Domenico, Lipari, Helga, Buscarino, Calogero, Cunsolo, Rosario, Cavallaro, Sebastiano
Format:	Journal Article
Language:	English
Published:	Netherlands 01-01-2011
Subjects:	Area Under Curve Biomarkers, Tumor - analysis Biomarkers, Tumor - metabolism Brain Neoplasms - metabolism Brain Neoplasms - secondary Carcinoma, Non-Small-Cell Lung - metabolism Carcinoma, Non-Small-Cell Lung - pathology Chemokine CXCL12 - analysis Chemokine CXCL12 - biosynthesis Female Fluorescent Antibody Technique Humans Lung Neoplasms - metabolism Lung Neoplasms - pathology Male Middle Aged Neoplasm Staging Prognosis Receptors, CXCR4 - analysis Receptors, CXCR4 - biosynthesis ROC Curve Sensitivity and Specificity
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Summary:	Synchronous or metachronous brain metastases (BMs) occur in about 33% of patients affected by non-small-cell lung cancer (NSCLC). To date, no reliable biological marker is able to identify patients who will develop BMs. In the present study, using a quantitative double-labeling immunofluorescence analysis, we evaluated the expression of chemokine CXCL12 and its receptor, CXCR4, in primary NSCLC histological specimens of patients with and without BMs. The immunoreactivity of CXCL12 and CXCR4 was significantly higher in NSCLC samples of patients with BMs. We performed Receiver Operating Characteristics (ROC) analysis in order to define optimal cut-off values for CXCL12 and CXCR4 immunoreactivity that could discriminate between NSCLC patients without and with BMs. ROC curves showed a good diagnostic accuracy and adequate predictive power for both CXCL12 and CXCR4. These findings suggest a possible role for the CXCL12/CXCR4 axis in the metastatic evolution of NSCLC, and its potential use as prognostic markers and drug targets.
ISSN:	1875-8592
DOI:	10.3233/CBM-2011-0232