Effects of natural flavones on membrane properties and citotoxicity of HeLa cells

Effects of natural flavones on membrane properties and citotoxicity of HeLa cells

The aim of this study was to determine whether eupafolin and hispidulin, flavones extracted from Eupatorium littorale Cabrera, Asteraceae, have the ability to change properties of biological membranes and promote cytotoxic effects. Eupafolin (50-200 µM) decreased approximately 30% the rate and total...

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Bibliographic Details
Published in:Revista brasileira de farmacognosia Vol. 20; no. 3; pp. 403 - 408
Main Authors: Herrerias, Tatiana, Oliveira, Alexandre A., Belem, Maurício L., Oliveira, Brás H., Carnieri, Eva G. S., Cadena, Sílvia M. S. C., Noleto, Guilhermina R., Martinez, Glaucia R., Oliveira, Maria B. M., Rocha, Maria E. M.
Format: Journal Article
Language:English
Published: Sociedade Brasileira de Farmacognosia 01-07-2010
Subjects:
PHARMACOLOGY & PHARMACY
inchamento mitocondrial
hispidulina
Eupafolina
hispidulin
HeLa cells
células HeLa
Eupafolin
mitochondrial swelling
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Summary:The aim of this study was to determine whether eupafolin and hispidulin, flavones extracted from Eupatorium littorale Cabrera, Asteraceae, have the ability to change properties of biological membranes and promote cytotoxic effects. Eupafolin (50-200 µM) decreased approximately 30% the rate and total amplitude of valinomycin induced swelling and 60-100% the energy-dependent mitochondrial swelling. Moreover, eupafolin (200 µM) reduced 35% the mitochondrial permeability transition, and hispidulin did not change this parameter in any of the doses tested. The evaluation of phase transition of DMPC liposomes with the probe DPH demonstrated that hispidulin and eupafolin affect gel and fluid phase. With mitochondrial membrane as model, hispidulin increased the polarization of fluorescence when used DPH-PA probe. Eupafolin and hispidulin (100 µM) promoted a reduction of 40% in cellular viability of HeLa cells in 24 h. Our results suggest that eupafolin and hispidulin have cytotoxic effects that can be explained, in part, by alterations promoted on biological membranes properties and mitochondrial bioenergetics.
ISSN:0102-695X
1981-528X
DOI:10.1590/S0102-695X2010000300018