Search Results - "Beldarraín, Alejandro"

Screening for stability and compatibility conditions of recombinant human epidermal growth factor for parenteral formulation: Effect of pH, buffers, and excipients by Santana, Héctor, González, Yaima, Campana, Patricia Targon, Noda, Jesús, Amarantes, Odalys, Itri, Rosangela, Beldarraín, Alejandro, Páez, Rolando

“…A successful parenteral formulation can be developed by studying stability and compatibility of biopharmaceuticals as a function of solution composition. Here,…”
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Stabilization of a recombinant human epidermal growth factor parenteral formulation through freeze-drying by Santana, Héctor, Sotolongo, Jorge, González, Yaima, Hernández, Gerardo, Chinea, Glay, Gerónimo, Haydée, Amarantes, Odalys, Beldarraín, Alejandro, Páez, Rolando

“…Development studies were performed to design a pharmaceutical composition that allows the stabilization of a parenteral rhEGF formulation in a lyophilized…”
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Conformational stability of apoflavodoxin by Genzor, Carlos G., Gómez‐Moreno, Carlos, Sancho, Javier, Beldarraín, Alejandro, López‐Lacomba, José Luis, Cortijo, Manuel

“…Flavodoxins are α/β proteins that mediate electron transfer reactions. The conformational stability of apoflavodoxin from Anabœna PCC 7119 has been studied by…”
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Aplicación del análisis de riesgo a la producción de proteínas recombinantes expresadas en Escherichia coli by García, José, Santana, Zeila, Zumalacárregui, Lourdes, Quintana, Marisel, Milá, Lorely, Ramos, Marbel, Beldarraín, Alejandro

“…En este trabajo se aplicó el análisis de riesgo, empleando la metodología de análisis de modos y efectos de fallas a los procesos de fermentación que utilizan…”
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Cromatografía de adsorción para purificar el disacárido espaciador del ingrediente farmacéutico activo de la vacuna QuimiHib by Díaz, Belinda, Heynngnezz, Lázaro, Beldarraín, Alejandro, Iser, Yadira, Fernández, Abel, Díaz, Jesús, Duarte, Rainier, García, Yuliasne, Martorell, Yaima, Sosa, Iván Manuel

“…En este trabajo se estudiaron las condiciones de la cromatografía de adsorción en Silicagel 60 para la purificación del disacárido espaciador (DSE), componente…”
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Optimisation of the coupled monoclonal antibody density for recombinant hepatitis B virus surface antigen immunopurification by Hernández, Raúl, Plana, Liuba, Gómez, Leonardo, Expósito, Néstor, Valdés, Jorge, Páez, Rolando, Martı́nez, Eduardo, Beldarraı́n, Alejandro

“…Using immunosorbents based upon cyanogen bromide-Sepharose CL-4B, we have examined different ligand densities in coupling of monoclonal antibody (MAb) to find…”
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Stability Studies of a Freeze-Dried Recombinant Human Epidermal Growth Factor Formulation for Wound Healing by Santana, Héctor, García, Gerardo, Vega, Maribel, Beldarraín, Alejandro, Páez, Rolando

“…We report on the stability assessment of a recombinant human epidermal growth factor (rhEGF) freeze-dried formulation for wound healing by intra-lesional…”
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Purification and conformational properties of a human interferon α2b produced in Escherichia coli by Beldarraín, Alejandro, Cruz, Yai, Cruz, Oscar, Navarro, Monica, Gil, Miriela

“…Recombinant human interferon α2b was expressed intracellularly in Escherichia coli as insoluble aggregates using a new expression vector, and was purified to…”
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Enzymic, spectroscopic and calorimetric studies of a recombinant dextranase expressed in Pichia pastoris by Beldarrain, A, Acosta, N, Betancourt, L, Gonzalez, L.J, Pons, T

“…Conformational stability and structural characterization of an rDex (recombinant dextranase) expressed in Pichia pastoris were studied by enzymic assays,…”
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Characterization of recombinant invertase expressed in methylotrophic yeasts by Acosta, Niuris, Beldarraín, Alejandro, Rodríguez, Luis, Alonso, Yamirka

“…We studied, for the first time, characterization of the invertase expressed in the methylotrophic yeasts Hansenula polymorpha and Pichia pastoris in terms of…”
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DNA removal from a purification process of recombinant hepatitis B surface antigen by Iznaga, Alejandro Beldarraín, Frontera, Mayda Candelario, Uramis, Javier Rodríguez, González, José Blas Tejera, Parra, Yodelis Calvo, González, Yoel Madruga

“…We studied the capacity of an API-rHBsAg purification process to eliminate DNA contamination from yeast-host cell. Firstly, was demonstrated consistency of…”
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Structural model for family 32 of glycosyl-hydrolase enzymes by Pons, Tirso, Olmea, Osvaldo, Chinea, Glay, Beldarraín, Alejandro, Márquez, Gabriel, Acosta, Niuris, Rodríguez, Luis, Valencia, Alfonso

“…A structural model is presented for family 32 of the glycosyl‐hydrolase enzymes based on the beta‐propeller fold. The model is derived from the common…”
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Characterization of Mucor pusillus rennin expressed in Pichia pastoris: enzymic, spectroscopic and calorimetric studies by Beldarraín, Alejandro, Acosta, Niuris, Montesinos, Raquel, Mata, Marlene, Cremata, José

“…The aspartic proteinase gene of Mucor pusillus rennin expressed in Pichia pastoris was characterized in terms of structural and conformational stability…”
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Structural model of Dex protein fromPenicillium minioluteumand its implications in the mechanism of catalysis by Pons, Tirso, Chinea, Glay, Olmea, Osvaldo, Beldarraín, Alejandro, Roca, Hernán, Padrón, Gabriel, Valencia, Alfonso

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Structural model of Dex protein from Penicillium minioluteumand its implications in the mechanism of catalysis by Pons, Tirso, Chinea, Glay, Olmea, Osvaldo, Beldarraín, Alejandro, Roca, Hernán, Padrón, Gabriel, Valencia, Alfonso

“…The DEX gene encodes an extracellular dextranase (EC 3.2.1.11); this enzyme hydrolyzes the α(1,6) glucosidic bond contained in dextran to release small…”
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Structural model of Dex protein from Penicillium minioluteum and its implications in the mechanism of catalysis by Pons, T, Chinea, G, Olmea, O, Beldarraín, A, Roca, H, Padrón, G, Valencia, A

“…The DEX gene encodes an extracellular dextranase (EC 3.2.1.11); this enzyme hydrolyzes the alpha(1,6) glucosidic bond contained in dextran to release small…”
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Multidomain structure of a recombinant streptokinase. A differential scanning calorimetry study by Beldarraín, A, López-Lacomba, J L, Kutyshenko, V P, Serrano, R, Cortijo, M

“…The temperature dependence of the heat capacity function of a recombinant streptokinase (rSK) has been studied by high-sensitivity differential scanning…”
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Análisis de riesgos de la calidad en un cambio de campaña. Etapa de limpieza by López García, Yohanix, Beldarraín Iznaga, Alejandro, Alegret Savinova, Oleg Evio, Álvarez Betancourt, Denis, García Sánchez, Carlos Arturo, Ramos Alfonso, Marbel, Hernández Marrero, Luciano

“…Objetivo: identificar los riesgos generales en la etapa de limpieza de los cambios de campaña de una planta multipropósito para productos biológicos, así como…”
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Evaluating sterilizing filtration for API-HBsAg by Alejandro Beldarraín, Yanelis Robainas, Nancy Pentón, Francis Hernández, Yamilé Mendoza, Manuel Rodríguez, Juana M Hernández, Lisbet Melo, Yenay Díaz, Soraya Piloto, César Quintero, Daylon Pérez, Boris Menéndez, Ayankay Vidal

“…The sterilizing filtration procedure for the active pharmaceutical ingredient of the Cuban anti-hepatitis B vaccine (HBsAg API) was evaluated by using…”
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Thermal Denaturation of Human γ-Interferon. A Calorimetric and Spectroscopic Study by Beldarrain, Alejandro, López-Lacomba, José Luis, Furrazola, Gustavo, Barberia, Daysi, Cortijo, Manuel

“…The thermal denaturation of a recombinant human γ-interferon has been studied as a function of pH in the range from 2 to 10 and buffer concentration in the…”
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