Search Results - "Belardi, Brian"

Turn-on protein switches for controlling actin binding in cells by Effiong, Unyime M., Khairandish, Hannah, Ramirez-Velez, Isabela, Wang, Yanran, Belardi, Brian

“…Within a shared cytoplasm, filamentous actin (F-actin) plays numerous and critical roles across the cell body. Cells rely on actin-binding proteins (ABPs) to…”
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Biased localization of actin binding proteins by actin filament conformation by Harris, Andrew R., Jreij, Pamela, Belardi, Brian, Joffe, Aaron M., Bausch, Andreas R., Fletcher, Daniel A.

“…The assembly of actin filaments into distinct cytoskeletal structures plays a critical role in cell physiology, but how proteins localize differentially to…”
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Azobenzene-based sinusoidal surface topography drives focal adhesion confinement and guides collective migration of epithelial cells by Fedele, Chiara, Mäntylä, Elina, Belardi, Brian, Hamkins-Indik, Tiama, Cavalli, Silvia, Netti, Paolo A., Fletcher, Daniel A., Nymark, Soile, Priimagi, Arri, Ihalainen, Teemu O.

“…Surface topography is a key parameter in regulating the morphology and behavior of single cells. At multicellular level, coordinated cell displacements drive…”
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Imaging the Glycosylation State of Cell Surface Glycoproteins by Two-Photon Fluorescence Lifetime Imaging Microscopy by Belardi, Brian, de la Zerda, Adam, Spiciarich, David R., Maund, Sophia L., Peehl, Donna M., Bertozzi, Carolyn R.

“…Glycoproteins in focus: Metabolic labeling of glycans with azido sugars (see picture) in combination with two‐photon fluorescence lifetime imaging microscopy…”
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Investigating Cell Surface Galectin-Mediated Cross-Linking on Glycoengineered Cells by Belardi, Brian, O’Donoghue, Geoff P, Smith, Adam W, Groves, Jay T, Bertozzi, Carolyn R

“…The galectin family of glycan-binding proteins is thought to mediate many cellular processes by oligomerizing cell surface glycoproteins and glycolipids into…”
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Extracellular Electron Transfer Enables Cellular Control of Cu(I)-Catalyzed Alkyne–Azide Cycloaddition by Partipilo, Gina, Graham, Austin J, Belardi, Brian, Keitz, Benjamin K

“…Extracellular electron transfer (EET) is an anaerobic respiration process that couples carbon oxidation to the reduction of metal species. In the presence of a…”
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Storming the gate: New approaches for targeting the dynamic tight junction for improved drug delivery by Ramirez-Velez, Isabela, Belardi, Brian

“…[Display omitted] As biologics used in the clinic outpace the number of new small molecule drugs, an important challenge for their efficacy and widespread use…”
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Cell–cell interfaces as specialized compartments directing cell function by Belardi, Brian, Son, Sungmin, Felce, James H., Dustin, Michael L., Fletcher, Daniel A.

“…Cell–cell interfaces are found throughout multicellular organisms, from transient interactions between motile immune cells to long-lived cell–cell contacts in…”
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A Weak Link with Actin Organizes Tight Junctions to Control Epithelial Permeability by Belardi, Brian, Hamkins-Indik, Tiama, Harris, Andrew R., Kim, Jeongmin, Xu, Ke, Fletcher, Daniel A.

“…In vertebrates, epithelial permeability is regulated by the tight junction (TJ) formed by specialized adhesive membrane proteins, adaptor proteins, and the…”
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Light-Activated Assembly of Connexon Nanopores in Synthetic Cells by Sihorwala, Ahmed Z., Lin, Alexander J., Stachowiak, Jeanne C., Belardi, Brian

“…During developmental processes and wound healing, activation of living cells occurs with spatiotemporal precision and leads to rapid release of soluble…”
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Chemical Lectinology: Tools for Probing the Ligands and Dynamics of Mammalian Lectins In Vivo by Belardi, Brian, Bertozzi, Carolyn R.

“…The importance and complexity associated with the totality of glycan structures, i.e. the glycome, has garnered significant attention from chemists and…”
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Paracellular Delivery of Protein Drugs with Smart EnteroPatho Nanoparticles by Ramirez-Velez, Isabela, Namjoshi, Aditya A., Effiong, Unyime M., Peppas, Nicholas A., Belardi, Brian

“…A general platform for the safe and effective oral delivery of biologics would revolutionize the administration of protein-based drugs, improving access for…”
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One and one is not two: taking a fresh look at membrane interfaces by Belardi, Brian, Fletcher, Daniel A.

“…Plasma membranes sit at the divide — conceptually and literally — between the interior and exterior milieu of cells, coordinating communication between nearby…”
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Molecular height measurement by cell surface optical profilometry (CSOP) by Son, Sungmin, Takatori, Sho C., Belardi, Brian, Podolski, Marija, Bakalar, Matthew H., Fletcher, Daniel A.

“…The physical dimensions of proteins and glycans on cell surfaces can critically affect cell function, for example, by preventing close contact between cells…”
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Steric pressure between glycosylated transmembrane proteins inhibits internalization by endocytosis by Gollapudi, Sadhana, Jamal, Sabah, Kamatar, Advika, Yuan, Feng, Wang, Liping, Lafer, Eileen M, Belardi, Brian, Stachowiak, Jeanne C

“…Clathrin-mediated endocytosis is essential for the removal of transmembrane proteins from the plasma membrane in all eukaryotic cells. Many transmembrane…”
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Claudin-4 reconstituted in unilamellar vesicles is sufficient to form tight interfaces that partition membrane proteins by Belardi, Brian, Son, Sungmin, Vahey, Michael D, Wang, Jinzhi, Hou, Jianghui, Fletcher, Daniel A

“…Tight junctions have been hypothesized to act as molecular fences in the plasma membrane of epithelial cells, helping to form differentiated apical and…”
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Steric regulation of tandem calponin homology domain actin-binding affinity by Harris, Andrew R, Belardi, Brian, Jreij, Pamela, Wei, Kathy, Shams, Hengameh, Bausch, Andreas, Fletcher, Daniel A

“…Tandem calponin homology (CH1-CH2) domains are common actin-binding domains in proteins that interact with and organize the actin cytoskeleton. Despite regions…”
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Engineering Tissue-Scale Properties with Synthetic Cells: Forging One from Many by Lin, Alexander J., Sihorwala, Ahmed Z., Belardi, Brian

“…In metazoans, living cells achieve capabilities beyond individual cell functionality by assembling into multicellular tissue structures. These higher-order…”
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Nuclear repartitioning of galectin-1 by an extracellular glycan switch regulates mammary morphogenesis by Bhat, Ramray, Belardi, Brian, Mori, Hidetoshi, Kuo, Peiwen, Tam, Andrew, Hines, William C., Le, Quynh-Thu, Bertozzi, Carolyn R., Bissell, Mina J.

“…Branching morphogenesis in the mammary gland is achieved by the migration of epithelial cells through a microenvironment consisting of stromal cells and…”
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Piperidine-based glycodendrons as protein N-glycan prosthetics by Hudak, Jason E., Belardi, Brian, Appel, Mason J., Solania, Angelo, Robinson, Peter V., Bertozzi, Carolyn R.

“…[Display omitted] The generation of homogeneously glycosylated proteins is essential for defining glycoform-specific activity and improving protein-based…”
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