Placental Dysferlin Expression is Reduced in Severe Preeclampsia

Abstract Dysferlin (DYSF) and myoferlin (MYOF), members of the ferlin family of membrane proteins, are co-expressed in human placental syncytiotrophoblast (STB). Although the role of these ferlin proteins in the placenta has yet to be established, it has been suggested that DYSF and MYOF may contrib...

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Bibliographic Details
Published in:	Placenta (Eastbourne) Vol. 30; no. 8; pp. 711 - 718
Main Authors:	Lang, C.T, Markham, K.B, Behrendt, N.J, Suarez, A.A, Samuels, P, Vandre, D.D, Robinson, J.M, Ackerman, W.E
Format:	Journal Article
Language:	English
Published:	Kidlington Elsevier Ltd 01-08-2009 Elsevier
Subjects:	Adolescent Adult Biological and medical sciences Calcium-Binding Proteins Case-Control Studies Cell Membrane - metabolism Cell-Derived Microparticles - metabolism Cross-Sectional Studies Down-Regulation Dysferlin Embryology: invertebrates and vertebrates. Teratology Female Fundamental and applied biological sciences. Psychology Humans Internal Medicine Membrane Proteins - genetics Membrane Proteins - metabolism Microscopy, Fluorescence Middle Aged Muscle Proteins - genetics Muscle Proteins - metabolism Myoferlin Obstetrics and Gynecology Placenta Placenta - metabolism Pre-Eclampsia - genetics Pre-Eclampsia - metabolism Preeclampsia Pregnancy RNA, Messenger - genetics RNA, Messenger - metabolism Tissue Distribution Trophoblasts - metabolism Young Adult Placenta Myoferlin Preeclampsia Dysferlin Vertebrata Mammalia Pregnancy disorders Fetal membrane Severe Pregnancy toxemia
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Summary:	Abstract Dysferlin (DYSF) and myoferlin (MYOF), members of the ferlin family of membrane proteins, are co-expressed in human placental syncytiotrophoblast (STB). Although the role of these ferlin proteins in the placenta has yet to be established, it has been suggested that DYSF and MYOF may contribute to the stability of the apical STB plasma membrane. The release of STB-derived cellular debris increases in the setting of preeclampsia (PE), suggesting relative destabilization of the hemochorial interface. To test whether PE was associated with alterations in placental expression of DYSF and/or MYOF, a cross-sectional study was performed using specimens of villous placenta collected form women with severe PE ( n = 10) and normotensive controls ( n = 10). DYSF and MYOF expression were examined using quantitative real–time RT-PCR, immunoblotting, and immunofluorescence labeling of tissue specimens. Placental DYSF expression was 57% lower at the mRNA level ( p = 0.03) and 38% lower at the protein level ( p = 0.026) in severe PE as compared to normotensive subjects. There were no differences in placental MYOF protein or mRNA expression between these groups. No appreciable changes in the distribution of DYSF or MYOF within placental villi was observed in PE relative to control specimens. We conclude that DYSF expression is reduced in severe PE relative to gestational age-matched controls. As DYSF has a role in membrane repair, these data suggest a role for DYSF in the stability of the apical STB plasma membrane and may account, at least in part, for the increased shedding of microparticles from this membrane in PE.
ISSN:	0143-4004 1532-3102
DOI:	10.1016/j.placenta.2009.05.008