Quercetin enhances adiponectin secretion by a PPAR-γ independent mechanism

To study possible insulin sensitizing, anti-inflammatory and anti-oxidative effects of the flavonol quercetin, rats were fed a high-fat diet (19%, w/w) with (HFQ) or without (HF) 0.03% quercetin or a flavonoid-poor low-fat (5%, w/w) maintenance diet (LF) over 4 weeks. Body weight was measured weekly...

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Bibliographic Details
Published in:	European journal of pharmaceutical sciences Vol. 41; no. 1; pp. 16 - 22
Main Authors:	Wein, Silvia, Behm, Norma, Petersen, Rasmus K., Kristiansen, Karsten, Wolffram, Siegfried
Format:	Journal Article
Language:	English
Published:	Kindlington Elsevier B.V 11-09-2010 Elsevier
Subjects:	Adiponectin Adiponectin - blood Adiponectin - secretion Animals Biological and medical sciences Blood Glucose - analysis Cells, Cultured Cholesterol - blood Chromatography, High Pressure Liquid Flavonoids General pharmacology Insulin - blood Leptin - blood Male Medical sciences Mice Oxidative Stress Peroxisome-proliferator-activated-receptor Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments PPAR gamma - genetics PPAR gamma - metabolism Quercetin Quercetin - pharmacology Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Triglycerides - blood Peroxisome-proliferator-activated-receptor Oxidative stress Flavonoids Quercetin Adiponectin Pharmaceutical technology Secretion PPAR-γ receptor Flavonoid Polyphenol Phenols Mechanism of action Peroxisome proliferator activated receptor
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Summary:	To study possible insulin sensitizing, anti-inflammatory and anti-oxidative effects of the flavonol quercetin, rats were fed a high-fat diet (19%, w/w) with (HFQ) or without (HF) 0.03% quercetin or a flavonoid-poor low-fat (5%, w/w) maintenance diet (LF) over 4 weeks. Body weight was measured weekly, and plasma concentrations of adiponectin, leptin, insulin, glucose, triacylglycerols, total cholesterol, as well as of markers of inflammation and oxidative stress were measured (12h fasted) at the end of the feeding period. Adiponectin and peroxisome-proliferator-activated-receptor (PPAR)-γ mRNA were measured in adipose tissue (WAT) by real-time RT-PCR. PPAR-γ transactivation was investigated by means of a reporter gene assay. HF feeding resulted in elevated fasted plasma glucose concentrations, while HFQ did not differ from LF feeding. In the HFQ group plasma concentrations and WAT mRNA levels of adiponectin were elevated compared with the HF group, however, PPAR-γ mRNA concentration in WAT was decreased (HFQ vs. HF). Compared to both other groups quercetin feeding significantly reduced oxidative stress, measured by plasma 8-iso-PGF2α, while body weight gain, body composition and plasma leptin levels were not affected. Neither quercetin nor its metabolites induced PPAR-γ-mediated transactivation in vitro. Adiponectin stimulating effects of quercetin are PPAR-γ-independent and prevent impairment of insulin sensitivity without affecting body weight and composition.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0928-0987 1879-0720
DOI:	10.1016/j.ejps.2010.05.004