Simple, rapid and on spot dye-based sensor for the detection of Vibrio load in shrimp culture farms

Simple, rapid and on spot dye-based sensor for the detection of Vibrio load in shrimp culture farms

For the detection of Vibrio bacteria, a kit involving two-step method was developed. In the in first step, a specific media was added in the water sample which selectively promote the growth of vibrios and inhibit the growth of other bacteria. The second step involved addition of dye-based sensor (a...

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Bibliographic Details
Published in:Archives of microbiology Vol. 203; no. 6; pp. 3525 - 3532
Main Authors: Kumar, Sweta Binod, Shinde, Ambika Hemant, Behere, Maheshawari Jagadishbhai, Italia, Dhruvi, Haldar, Soumya
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-08-2021
Springer Nature B.V
Subjects:
Aquaculture
Bacteria
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cell Biology
Coliforms
Dyes
E coli
Ecology
Farms
Life Sciences
Microbial Ecology
Microbiology
Original Paper
Ponds
Probiotics
Shellfish farming
Shrimp culture
Vibrio
Water analysis
Water sampling
Water
Probiotics
Detection
Kit
Aquaculture
Vibrio
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Summary:For the detection of Vibrio bacteria, a kit involving two-step method was developed. In the in first step, a specific media was added in the water sample which selectively promote the growth of vibrios and inhibit the growth of other bacteria. The second step involved addition of dye-based sensor (already developed in our previous work) in the sample which detect the active Vibrio and changed the colour of the sample to red/pink. The vibrio detection kit was optimised on five different species of Vibrio ( V. cholerae , V. parahaemolyticus , V. campbellii , V. harveyi and V. proteolyticus ) and two negative control bacteria ( Escherichia coli and Bacillus subtilis ). The kit was further evaluated on aquaculture pond water and probiotics used in aquaculture farms. It successfully estimated Vibrio concentration of all the five strains in aquaculture ponds. The negative control bacteria and probiotics were not sensed by the kit. Hence, the kit developed here is perfect for the detection of Vibrio , especially in aquaculture farms.
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ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-021-02333-3