Search Results - "Begley, Tadhg"

Mechanistic Studies on CysS - A Vitamin B 12 -Dependent Radical SAM Methyltransferase Involved in the Biosynthesis of the tert -Butyl Group of Cystobactamid by Wang, Yuanyou, Begley, Tadhg P

“…Cobalamin (Cbl)-dependent radical -adenosylmethionine (SAM) methyltransferases catalyze methylation reactions at non-nucleophilic centers in a wide range of…”
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Alkylcysteine Sulfoxide C–S Monooxygenase Uses a Flavin-Dependent Pummerer Rearrangement by Hazra, Sohan, Begley, Tadhg P.

“…Flavoenzymes are highly versatile and participate in the catalysis of a wide range of reactions, including key reactions in the metabolism of sulfur-containing…”
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Dibenzothiophene Catabolism Proceeds via a Flavin-N5-oxide Intermediate by Adak, Sanjoy, Begley, Tadhg P

“…The dibenzothiophene catabolic pathway converts dibenzothiophene to 2-hydroxybiphenyl and sulfite. The third step of the pathway, involving the conversion of…”
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RutA-Catalyzed Oxidative Cleavage of the Uracil Amide Involves Formation of a Flavin-N5-oxide by Adak, Sanjoy, Begley, Tadhg P

“…RutA is a novel flavoenzyme on the uracil catabolic pathway that catalyzes uracil ring opening by a unique amide oxidation reaction. Here we provide evidence…”
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Hexachlorobenzene Catabolism Involves a Nucleophilic Aromatic Substitution and Flavin-N5-Oxide Formation by Adak, Sanjoy, Begley, Tadhg P

“…HcbA1 is a unique flavoenzyme that catalyzes the first step in the bacterial hexachlorobenzene catabolic pathway. Here we report in vitro reconstitution of the…”
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Mechanistic Studies on CysS - A Vitamin B12-Dependent Radical SAM Methyltransferase Involved in the Biosynthesis of the tert-Butyl Group of Cystobactamid by Wang, Yuanyou, Begley, Tadhg P

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Flavin-N5-oxide: A new, catalytic motif in flavoenzymology by Adak, Sanjoy, Begley, Tadhg P.

“…Flavin-N5-oxide is a recently discovered intermediate used by EncM (1,3-diketone oxidation), DszA (sulfone monooxygenase) and RutA (amide monooxygenase). This…”
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Radical SAM Enzyme HydE Generates Adenosylated Fe(I) Intermediates En Route to the [FeFe]-Hydrogenase Catalytic H-Cluster by Tao, Lizhi, Pattenaude, Scott A, Joshi, Sumedh, Begley, Tadhg P, Rauchfuss, Thomas B, Britt, R David

“…The H-cluster of [FeFe]-hydrogenase consists of a [4Fe-4S] -subcluster linked by a cysteinyl bridge to a unique organometallic [2Fe] -subcluster assigned as…”
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Mechanistic Studies on the Single-Turnover Yeast Thiamin Pyrimidine Synthase: Characterization of the Inactive Enzyme by Lai, Rung-Yi, Mondal, Anushree, Fedoseyenko, Dmytro, Begley, Tadhg P.

“…The eukaryotic thiamin pyrimidine synthase, THI5p, has been identified as a suicidal/single-turnover enzyme that catalyzes the conversion of its active site…”
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The structural and biochemical foundations of thiamin biosynthesis by Jurgenson, Christopher T, Begley, Tadhg P, Ealick, Steven E

“…Thiamin is synthesized by most prokaryotes and by eukaryotes such as yeast and plants. In all cases, the thiazole and pyrimidine moieties are synthesized in…”
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Biochemistry: Origin of a key player in methane biosynthesis by Begley, Tadhg P

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Menaquinone Biosynthesis: Formation of Aminofutalosine Requires a Unique Radical SAM Enzyme by Mahanta, Nilkamal, Fedoseyenko, Dmytro, Dairi, Tohru, Begley, Tadhg P

“…Menaquinone (MK, vitamin K2) is a lipid-soluble molecule that participates in the bacterial electron transport chain. In mammalian cells, MK functions as an…”
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Tryptophan Lyase (NosL): A Cornucopia of 5′-Deoxyadenosyl Radical Mediated Transformations by Bhandari, Dhananjay M, Fedoseyenko, Dmytro, Begley, Tadhg P

“…Tryptophan lyase (NosL) is a radical S-adenosyl-l-methionine (SAM) enzyme that catalyzes the formation of 3-methyl-2-indolic acid from l-tryptophan. In this…”
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Oxidative Dearomatization of PLP in Thiamin Pyrimidine Biosynthesis in Candida albicans by Mondal, Anushree, Lai, Rung-Yi, Fedoseyenko, Dmytro, Giri, Nitai, Begley, Tadhg P.

“…The yeast thiamin pyrimidine synthase THI5p catalyzes one of the most complex organic rearrangements found in primary metabolism. In this reaction, the active…”
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Novel enzymology in futalosine-dependent menaquinone biosynthesis by Joshi, Sumedh, Fedoseyenko, Dmytro, Mahanta, Nilkamal, Manion, Hannah, Naseem, Saad, Dairi, Tohru, Begley, Tadhg P

“…The recently discovered futalosine-dependent menaquinone biosynthesis pathway employs radical chemistry for the naphthoquinol core assembly. Mechanistic…”
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Biosynthesis of Branched Alkoxy Groups: Iterative Methyl Group Alkylation by a Cobalamin-Dependent Radical SAM Enzyme by Wang, Yuanyou, Schnell, Bastien, Baumann, Sascha, Müller, Rolf, Begley, Tadhg P

“…The biosynthesis of branched alkoxy groups, such as the unique t-butyl group found in a variety of natural products, is still poorly understood. Recently,…”
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Mechanistic Studies on Tryptophan Lyase (NosL): Identification of Cyanide as a Reaction Product by Bhandari, Dhananjay M, Fedoseyenko, Dmytro, Begley, Tadhg P

“…Tryptophan lyase (NosL) catalyzes the formation of 3-methylindole-2-carboxylic acid and 3-methylindole from l-tryptophan. In this paper, we provide evidence…”
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Cysteine Dealkylation in Bacillus subtilis by a Novel Flavin-Dependent Monooxygenase by Hazra, Sohan, Bhandari, Dhananjay M., Krishnamoorthy, Kalyanaraman, Sekowska, Agnieszka, Danchin, Antoine, Begley, Tadhg P.

“…In this paper, we describe the biochemical reconstitution of a cysteine salvage pathway and the biochemical characterization of each of the five enzymes…”
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Aminofutalosine Synthase: Evidence for Captodative and Aryl Radical Intermediates Using β‑Scission and SRN1 Trapping Reactions by Joshi, Sumedh, Mahanta, Nilkamal, Fedoseyenko, Dmytro, Williams, Howard, Begley, Tadhg P

“…Aminofutalosine synthase (MqnE) is a radical SAM enzyme involved in the menaquinone biosynthetic pathway. In this communication, we propose a novel mechanism…”
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Biosynthetic Versatility and Coordinated Action of 5′-Deoxyadenosyl Radicals in Deazaflavin Biosynthesis by Philmus, Benjamin, Decamps, Laure, Berteau, Olivier, Begley, Tadhg P

“…Coenzyme F420 is a redox cofactor found in methanogens and in various actinobacteria. Despite the major biological importance of this cofactor, the…”
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