Search Results - "Beekman, M J"

Mutation-specific reporter for optimization and enrichment of prime editing by Schene, I. F., Joore, I. P., Baijens, J. H. L., Stevelink, R., Kok, G., Shehata, S., Ilcken, E. F., Nieuwenhuis, E. C. M., Bolhuis, D. P., van Rees, R. C. M., Spelier, S. A., van der Doef, H. P. J., Beekman, J. M., Houwen, R. H. J., Nieuwenhuis, E. E. S., Fuchs, S. A.

“…Prime editing is a versatile genome-editing technique that shows great promise for the generation and repair of patient mutations. However, some genomic sites…”
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Syntenin-mediated regulation of Sox4 proteasomal degradation modulates transcriptional output by Beekman, J M, Vervoort, S J, Dekkers, F, van Vessem, M E, Vendelbosch, S, Brugulat-Panès, A, van Loosdregt, J, Braat, A K, Coffer, P J

“…The transcription factor Sox4 is aberrantly expressed in many human tumors and can modulate tumorigenesis and metastases of murine tumors in vivo. However,…”
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Intestinal organoids for Cystic Fibrosis research by de Poel, E., Lefferts, J.W., Beekman, J.M.

“…Significant progress has been made in the development of CFTR modulator therapy; however, current CFTR modulator therapies are only available for a minority of…”
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Transcriptional activation by the estrogen receptor requires a conformational change in the ligand binding domain by Beekman, J M, Allan, G F, Tsai, S Y, Tsai, M J, O'Malley, B W

“…The estrogen receptor (ER) is a strong hormone-inducible transcription factor that regulates the expression of many genes. It was shown for the human…”
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Functional Restoration of CFTR Nonsense Mutations in Intestinal Organoids by de Poel, E., Spelier, S., Suen, S.W.F., Kruisselbrink, E., Graeber, S.Y., Mall, M.A., Weersink, E.J.M., van der Eerden, M.M., Koppelman, G.H., van der Ent, C.K., Beekman, J.M.

“…•Pharmacological repair of CFTR function beyond F508del/F508del-VX809/VX770 in intestinal organoids with homozygous PTC mutations.•The read-through agent…”
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Forskolin induced swelling (FIS) assay in intestinal organoids to guide eligibility for compassionate use treatment in a CF patient with a rare genotype by Aalbers, B.L., Brunsveld, J.E., van der Ent, C.K., van den Eijnden, J.C., Beekman, J.M., Heijerman, H.G.M.

“…•Intestinal organoids were used to identify a rare CFTR mutation as minimal function.•Based on this minimal function, a patient was accepted to a compassionate…”
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Members of the steroid hormone receptor superfamily interact with TFIIB (S300-II) by Ing, N H, Beekman, J M, Tsai, S Y, Tsai, M J, O'Malley, B W

“…The S300-II factor was discovered as an activator of ovalbumin gene transcription with the chicken ovalbumin upstream promoter-transcription factor (COUP-TF,…”
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FDA-approved drug screening in patient-derived organoids demonstrates potential of drug repurposing for rare cystic fibrosis genotypes by de Poel, E., Spelier, S., Hagemeijer, M.C., van Mourik, P., Suen, S.W.F., Vonk, A.M., Brunsveld, J.E., Ithakisiou, G.N., Kruisselbrink, E., Oppelaar, H., Berkers, G., de Winter de Groot, K.M., Heida-Michel, S., Jans, S.R., van Panhuis, H., Bakker, M., van der Meer, R., Roukema, J., Dompeling, E., Weersink, E.J.M., Koppelman, G.H., Blaazer, A.R., Muijlwijk-Koezen, J.E., van der Ent, C.K., Beekman, J.M.

“…•We implemented a high-throughput 384-wells version of the functional FIS assay to screen a large number of PDIOs for compounds that enhance CFTR function.•We…”
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FcγRIII stimulation breaks the tolerance of human nasal epithelial cells to bacteria through cross-talk with TLR4 by Golebski, K., Hoepel, W., van Egmond, D., de Groot, E. J., Amatngalim, G. D., Beekman, J. M., Fokkens, W. J., van Drunen, C. M., den Dunnen, J.

“…The nasal cavity displays immune tolerance to commensal bacteria under homeostatic conditions, which is rapidly converted to a pro-inflammatory response upon…”
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Targeted locus amplification reveals heterogeneity between and within CFTR genotypes and association with CFTR function in patient-derived intestinal organoids by Lefferts, J.W., Boersma, V., Nieuwenhuijze, N.D.A., Suen, S.W.F., Hajo, K., Collantes, N. Sanchez, Vermeulen, C., Groeneweg, T., Hagemeijer, M.C., de Jonge, H.R., van der Ent, C.K., Splinter, E., Beekman, J.M.

“…•Targeted locus amplification (TLA) allows for the allele-specific analysis of the complete CFTR gene.•Paired TLA and functional CFTR measurements in…”
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No proarrhythmic properties of the antibiotics Moxifloxacin or Azithromycin in anaesthetized dogs with chronic‐AV block by Thomsen, M B, Beekman, J D M, Attevelt, N J M, Takahara, A, Sugiyama, A, Chiba, K, Vos, M A

“…Background & purpose: The therapeutically available quinolone antibiotic moxifloxacin has been used as a positive control for prolonging the QT interval in…”
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Limited premature termination codon suppression by read-through agents in cystic fibrosis intestinal organoids by Zomer-van Ommen, D.D, Vijftigschild, L.A.W, Kruisselbrink, E, Vonk, A.M, Dekkers, J.F, Janssens, H.M, de Winter-de Groot, K.M, van der Ent, C.K, Beekman, J.M

“…Abstract Premature termination codon read-through drugs offer opportunities for treatment of multiple rare genetic diseases including cystic fibrosis. We here…”
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A bioassay using intestinal organoids to measure CFTR modulators in human plasma by Dekkers, R, Vijftigschild, L.A.W, Vonk, A.M, Kruisselbrink, E, de Winter-de Groot, K.M, Janssens, H.M, van der Ent, C.K, Beekman, J.M

“…Abstract Treatment efficacies of drugs depend on patient-specific pharmacokinetic and pharmacodynamic properties. Here, we developed an assay to measure…”
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CFTR-mutation specific applications of CFTR-directed monoclonal antibodies by van Meegen, M.A, Terheggen, S.W.J, Koymans, K.J, Vijftigschild, L.A.W, Dekkers, J.F, van der Ent, C.K, Beekman, J.M

“…Abstract Background Over the last decade novel monoclonal CFTR-specific antibodies have been developed. We here present a paired analysis to detect wild-type…”
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In vivo transcription of a progesterone-responsive gene is specifically inhibited by a triplex-forming oligonucleotide by Ing, Nancy H., Beekman, Johanna M., Kessler, Donald J., Murphy, Mark, Jayaraman, Krishna, Zendegui, Joseph G., Hogan, Michael E., O'Malley, Bert W., Tsai, Ming-Jer

“…Oligonucleotides provide novel reagents for Inhibition of gene expression because of their high affinity binding to specific nucleotide sequences. We describe…”
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Over-prescription of short-acting β2-agonists for asthma in South Africa: Results from the SABINA III study by Smith, C, Ambaram, A, Mitha, E, Abdullah, I, Abdullah, I A, Reddy, J, Trokis, J, Ramlachan, P, Govind, U, Lightfoot, K, Moodley, K, Smit, R, Beekman, M J H I

“…Background. Asthma medication prescription trends, including those of short-acting β2-agonists (SABAs), are not well documented for South Africa…”
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Evaluation of a novel assay of potential toxicity/neurotoxicity of carpet emissions (VOCs) in mice by Stott, W.T., Beekman, M.J., Johnson, K.A., Spencer, P.J.

“…A private testing laboratory utilizing the whole-body plethysmograph/head-only exposure apparatus outlined in the respiratory irritation assay ASTM E981-84,…”
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Hormone activation of baculovirus expressed progesterone receptors by ELLISTON, J. F, BEEKMAN, J. M, TSAI, S. Y, O'MALLEY, B. W, TSAI, M. J

“…Human and chicken progesterone receptors (A form) were overproduced in a baculovirus expression system. These recombinant progesterone receptors were…”
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4-Phenylcyclohexene: 2-week inhalation toxicity and neurotoxicity studies in swiss-webster mice by Beekman, M.J., Maurissen, J.P., Johnson, K.A.

“…4-Phenylcyclohexene (4-PCH) is a by-product formed during the polymerization of styrene-butadiene latex used in carpet backing. Limited reports suggest that…”
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MHC Class I Antigen Processing of an Adenovirus CTL Epitope Is Linked to the Levels of Immunoproteasomes in Infected Cells by Sijts, Alice J. A. M, Standera, Sybille, Toes, Rene E. M, Ruppert, Thomas, Beekman, Nico J. C. M, van Veelen, Peter A, Ossendorp, Ferry A, Melief, Cornelis J. M, Kloetzel, Peter M

“…Proteasomes are the major source for the generation of peptides bound by MHC class I molecules. To study the functional relevance of the IFN-gamma-inducible…”
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