Search Results - "Beebe, Emily T"

Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function by Floyd, Brendan J., Wilkerson, Emily M., Veling, Mike T., Minogue, Catie E., Xia, Chuanwu, Beebe, Emily T., Wrobel, Russell L., Cho, Holly, Kremer, Laura S., Alston, Charlotte L., Gromek, Katarzyna A., Dolan, Brendan K., Ulbrich, Arne, Stefely, Jonathan A., Bohl, Sarah L., Werner, Kelly M., Jochem, Adam, Westphall, Michael S., Rensvold, Jarred W., Taylor, Robert W., Prokisch, Holger, Kim, Jung-Ja P., Coon, Joshua J., Pagliarini, David J.

“…Mitochondria are essential for numerous cellular processes, yet hundreds of their proteins lack robust functional annotation. To reveal functions for these…”
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Identification and characterization of a set of monocot BAHD monolignol transferases by Smith, Rebecca A, Beebe, Emily T, Bingman, Craig A, Vander Meulen, Kirk, Eugene, Alexis, Steiner, Alexander J, Karlen, Steven D, Ralph, John, Fox, Brian G

“…Plant BAHD acyltransferases perform a wide range of enzymatic tasks in primary and secondary metabolism. Acyl-CoA monolignol transferases, which couple a CoA…”
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Mitochondrial COQ9 is a lipid-binding protein that associates with COQ7 to enable coenzyme Q biosynthesis by Lohman, Danielle C, Forouhar, Farhad, Beebe, Emily T, Stefely, Matthew S, Minogue, Catherine E, Ulbrich, Arne, Stefely, Jonathan A, Sukumar, Shravan, Luna-Sánchez, Marta, Jochem, Adam, Lew, Scott, Seetharaman, Jayaraman, Xiao, Rong, Wang, Huang, Westphall, Michael S, Wrobel, Russell L, Everett, John K, Mitchell, Julie C, López, Luis C, Coon, Joshua J, Tong, Liang, Pagliarini, David J

“…Coenzyme Q (CoQ) is an isoprenylated quinone that is essential for cellular respiration and is synthesized in mitochondria by the combined action of at least…”
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A bacterial biosynthetic pathway for methylated furan fatty acids by Lemke, Rachelle A.S., Olson, Stephanie M., Morse, Kaitlin, Karlen, Steven D., Higbee, Alan, Beebe, Emily T., Ralph, John, Coon, Joshua J., Fox, Brian G., Donohue, Timothy J.

“…Fatty acids play many important roles in cells and also in industrial processes. Furan fatty acids (FuFAs) are present in the lipids of some plant, fish, and…”
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Robotic large-scale application of wheat cell-free translation to structural studies including membrane proteins by Beebe, Emily T., Makino, Shin-ichi, Nozawa, Akira, Matsubara, Yuko, Frederick, Ronnie O., Primm, John G., Goren, Michael A., Fox, Brian G.

“…The use of the Protemist XE, an automated discontinuous-batch protein synthesis robot, in cell-free translation is reported. The soluble Galdieria sulphuraria…”
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pHBMT1, a BAHD-family monolignol acyltransferase, mediates lignin acylation in poplar by de Vries, Lisanne, MacKay, Heather A, Smith, Rebecca A, Mottiar, Yaseen, Karlen, Steven D, Unda, Faride, Muirragui, Emilia, Bingman, Craig, Vander Meulen, Kirk, Beebe, Emily T, Fox, Brian G, Ralph, John, Mansfield, Shawn D

“…Poplar (Populus) lignin is naturally acylated with p-hydroxybenzoate ester moieties. However, the enzyme(s) involved in the biosynthesis of the…”
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Mannose- and Mannobiose-Specific Responses of the Insect-Associated Cellulolytic Bacterium Streptomyces sp. Strain SirexAA-E by Ohashi, Keisuke, Hataya, Shogo, Nakata, Akane, Matsumoto, Kazuki, Kato, Natsumi, Sato, Wakana, Carlos-Shanley, Camila, Beebe, Emily T, Currie, Cameron R, Fox, Brian G, Takasuka, Taichi E

“…The cellulolytic insect symbiont bacterium sp. strain SirexAA-E secretes a suite of arbohydrate- ctive en ymes (CAZymes), which are involved in the degradation…”
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An Isoprene Lipid-Binding Protein Promotes Eukaryotic Coenzyme Q Biosynthesis by Lohman, Danielle C., Aydin, Deniz, Von Bank, Helaina C., Smith, Robert W., Linke, Vanessa, Weisenhorn, Erin, McDevitt, Molly T., Hutchins, Paul, Wilkerson, Emily M., Wancewicz, Benjamin, Russell, Jason, Stefely, Matthew S., Beebe, Emily T., Jochem, Adam, Coon, Joshua J., Bingman, Craig A., Dal Peraro, Matteo, Pagliarini, David J.

“…The biosynthesis of coenzyme Q presents a paradigm for how cells surmount hydrophobic barriers in lipid biology. In eukaryotes, CoQ precursors—among nature’s…”
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Quantitative Analysis of The High‐Yield Hydrolysis of Kelp by Laminarinase and Alginate Lyase by Takasuka, Taichi E., Kim, Hoon, Deng, Kai, Bianchetti, Christopher M., Yamashita, Kaho, Beebe, Emily T., Bergeman, Lai F., Vander Meulen, Kirk A., Deutsch, Samuel, Ralph, John, Adams, Paul D., Northen, Trent R., Fox, Brian G.

“…Kelp is an abundant, farmable biomass‐containing laminarin and alginate as major polysaccharides, providing an excellent model substrate to study their…”
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A cell-free method for expressing and reconstituting membrane proteins enables functional characterization of the plant receptor-like protein kinase FERONIA by Minkoff, Benjamin B., Makino, Shin-ichi, Haruta, Miyoshi, Beebe, Emily T., Wrobel, Russell L., Fox, Brian G., Sussman, Michael R.

“…There are more than 600 receptor-like kinases (RLKs) in Arabidopsis, but due to challenges associated with the characterization of membrane proteins, only a…”
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Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling by Walker, Johnnie A, Pattathil, Sivakumar, Bergeman, Lai F, Beebe, Emily T, Deng, Kai, Mirzai, Maryam, Northen, Trent R, Hahn, Michael G, Fox, Brian G

“…Glycoside hydrolases (GHs) are enzymes that hydrolyze polysaccharides into simple sugars. To better understand the specificity of enzyme hydrolysis within the…”
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A heterodimeric glutathione S-transferase that stereospecifically breaks lignin's β(R)-aryl ether bond reveals the diversity of bacterial β-etherases by Kontur, Wayne S., Olmsted, Charles N., Yusko, Larissa M., Niles, Alyssa V., Walters, Kevin A., Beebe, Emily T., Vander Meulen, Kirk A., Karlen, Steven D., Gall, Daniel L., Noguera, Daniel R., Donohue, Timothy J.

“…Lignin is a heterogeneous polymer of aromatic subunits that is a major component of lignocellulosic plant biomass. Understanding how microorganisms deconstruct…”
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Function of Shaker potassium channels produced by cell-free translation upon injection into Xenopus oocytes by Jarecki, Brian W., Makino, Shin-ichi, Beebe, Emily T., Fox, Brian G., Chanda, Baron

“…Voltage-gated ion channels are a class of membrane proteins that temporally orchestrate the ion flux critical for chemical and electrical signaling in…”
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The Serine-Rich N-Terminal Domain of Oat Phytochrome a Helps Regulate Light Responses and Subnuclear Localization of the Photoreceptor by Casal, Jorge J., Davis, Seth J., Kirchenbauer, Daniel, Viczian, Andras, Yanovsky, Marcelo J., Richard C. Clough, Kircher, Stefan, Emily T. Jordan-Beebe, Schäfer, Eberhard, Nagy, Ferenc, Vierstra, Richard D.

“…Phytochrome (phy) A mediates two distinct photobiological responses in plants: the very-low-fluence response (VLFR), which can be saturated by short pulses of…”
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Cell-free translation and purification of Arabidopsis thaliana regulator of G signaling 1 protein by Li, Bo, Makino, Shin-ichi, Beebe, Emily T., Urano, Daisuke, Aceti, David J., Misenheimer, Tina M., Peters, Jonathan, Fox, Brian G., Jones, Alan M.

“…Arabidopsis thaliana Regulator of G protein Signalling 1 (AtRGS1) is a protein with a predicted N-terminal 7-transmembrane (7TM) domain and a C-terminal…”
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Automated cell-free protein production methods for structural studies by Beebe, Emily T, Makino, Shin-Ichi, Markley, John L, Fox, Brian G

“…In contrast to cell-based protein expression, cell-free production is highly consistent, scalable, and amenable to automation. Robots can handle many samples…”
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Structural and Functional Characterization of CalS11, a TDP-Rhamnose 3′‑O‑Methyltransferase Involved in Calicheamicin Biosynthesis by Singh, Shanteri, Chang, Aram, Helmich, Kate E, Bingman, Craig A, Wrobel, Russell L, Beebe, Emily T, Makino, Shin-ichi, Aceti, David J, Dyer, Kevin, Hura, Greg L, Sunkara, Manjula, Morris, Andrew J, Phillips, George N, Thorson, Jon S

“…Sugar methyltransferases (MTs) are an important class of tailoring enzymes that catalyze the transfer of a methyl group from S-adenosyl-l-methionine to…”
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Cell‐free production of integral membrane aspartic acid proteases reveals zinc‐dependent methyltransferase activity of the Pseudomonas aeruginosa prepilin peptidase PilD by Aly, Khaled A., Beebe, Emily T., Chan, Chi H., Goren, Michael A., Sepúlveda, Carolina, Makino, Shin‐ichi, Fox, Brian G., Forest, Katrina T.

“…Integral membrane aspartic acid proteases are receiving growing recognition for their fundamental roles in cellular physiology of eukaryotes and prokaryotes,…”
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Cell-free protein synthesis for functional and structural studies by Makino, Shin-ichi, Beebe, Emily T, Markley, John L, Fox, Brian G

“…Recent advances in cell-free protein expression systems have made them reliable and practical for functional and structural studies of a wide variety of…”
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Cell‐free production of integral membrane aspartic acid proteases reveals zinc‐dependent methyltransferase activity of the P seudomonas aeruginosa prepilin peptidase PilD by Aly, Khaled A., Beebe, Emily T., Chan, Chi H., Goren, Michael A., Sepúlveda, Carolina, Makino, Shin‐ichi, Fox, Brian G., Forest, Katrina T.

“…Abstract Integral membrane aspartic acid proteases are receiving growing recognition for their fundamental roles in cellular physiology of eukaryotes and…”
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