Killer cell immunoglobulin-like receptor alleles influence susceptibility to occult hepatitis B infection in West African population
Occult hepatitis B infection (OBI) is a public health problem in Burkina Faso. OBI represents a risk factor for the development of cirrhosis and hepatocellular carcinoma (HCC). OBI could be due to mutant viruses undetectable by HBsAg assays or a strong suppression of viral replication and gene expre...
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| Published in: | Journal of public health in Africa Vol. 14; no. 9; p. 8 |
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| Main Authors: | , , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
PAGEPress Publications, Pavia, Italy
01-10-2023
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Occult hepatitis B infection (OBI) is a public health problem in Burkina Faso. OBI represents a risk factor for the development of cirrhosis and hepatocellular carcinoma (HCC). OBI could be due to mutant viruses undetectable by HBsAg assays or a strong suppression of viral replication and gene expression under the pression of the host immune system. To investigate the role of killer cell immunoglobulin‑like receptor (KIR) gene polymorphisms in patients with OBI in Burkina Faso compared to healthy and chronic hepatitis B subjects. A total of 286 participants was recruited, including 42 cases of OBI, 110 cases of chronic hepatitis B and 134 HBV negative subjects. SSP‑PCR was performed to search for the presence of KIR genes. The HBV viral load was determined by qPCR. The frequencies of the activator gene KIR2DS5 (P=0.045) and the pseudogene KIR2DP1 (P0.001) in patients with OBI were higher than those in patients with chronic hepatitis B. These genes are associated with susceptibility of occult hepatitis B infection. The frequencies of the inhibitory KIR gene KIR2DL3 (P=0.01) of patients with occult hepatitis B were lower than those in chronic hepatitis B patients. This gene KIR2DL3 is associated with protection against occult hepatitis B infection. Also, the frequencies of the inhibitory KIR genes KIR2DL2 (P0.001), KIR2DL3 (P0.001) and activators KIR2DS2 (P0.001) in chronic hepatitis B patients were higher compared to the frequencies of the KIR genes in healthy subjects. These genes KIR2DL3, KIR2DL5 (A, B), KIR3DL3, KIR3DS1, KIR2DL2 and KIR2DS2 are thought to be genes associated with the susceptibility to OBI. The KIR2DS5 and KIR2DP1 genes could be associated with susceptibility to OBI. As for the KIR gene KIR2DL3 could be associated with protection against occult hepatitis B infection. |
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| Bibliography: | The authors declare no potential conflict of interest. Contributions FWD, MS, JKS, study concept and design; MMB, PAS, NK, HKS, PB, ETY, ITK, sampling and laboratory analysis; AKO, PAS, MMB, statistical analysis and interpretation of data; FWD, MMB, MBN, ATY, drafting of the manuscript; FWD, MBN, DOY, JKS, critical revision of the manuscript for important intellectual content; ATY, JS, administrative, technical, and material support; FWD, JS, study supervision. All authors have read and approved the manuscript. Availability of data and materials All the participants gave informed consent for data sharing and the data presented in this paper are anonymized. Conflict of interest Ethics approval and consent to participate Informed consent Abbreviations: KIR, Killer cell immunoglobulin-like receptors; OBI, occult hepatitis B infection; HBsAg, hepatitis B surface antigen; HBV, hepatitis B virus; SSP-PCR, single specific primer-polymerase chain reaction The protocol for this research was approved by the Ethics Committee for Health Research (CERS) of Burkina Faso by deliberation number 2017-01-004 of January 11, 2017. Written informed consent was obtained from all the patients. All the data supported our finding are contained within this work. The datasets analyzed during the current study are available from the corresponding author upon request. |
| ISSN: | 2038-9922 2038-9930 |
| DOI: | 10.4081/jphia.2023.2586 |