A nationwide multicentre study in Turkey for establishing reference intervals of haematological parameters with novel use of a panel of whole blood

A nationwide multicentre study in Turkey for establishing reference intervals of haematological parameters with novel use of a panel of whole blood

A nationwide multicentre study was conducted to establish well-defined reference intervals (RIs) of haematological parameters for the Turkish population in consideration of sources of variation in reference values (RVs). K2-EDTA whole blood samples (total of 3363) were collected from 12 laboratories...

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Published in:Biochemia Medica Vol. 27; no. 2; pp. 350 - 377
Main Authors: Ozarda, Yesim, Ichihara, Kiyoshi, Bakan, Ebubekir, Polat, Harun, Ozturk, Nurinnisa, Baygutalp, Nurcan K, Taneli, Fatma, Guvenc, Yesim, Ormen, Murat, Erbayraktar, Zubeyde, Aksoy, Nurten, Sezen, Hatice, Demir, Meltem, Eskandari, Gulcin, Polat, Gurbuz, Mete, Nuriye, Yuksel, Hatice, Vatansev, Husamettin, Gun, Fatma, Akin, Okhan, Ceylan, Ozlem, Noyan, Tevfik, Gozlukaya, Ozgul, Aliyazicioglu, Yuksel, Kahraman, Sevim, Dirican, Melahat, Tuncer, Gul Ozlem, Kimura, Shogo, Eker, Pinar
Format: Journal Article Paper
Language:English
Published: Croatia Medicinska naklada 15-06-2017
Croatian Society of Medical Biochemistry and Laboratory Medicine
Subjects:
Adolescent
Adult
Aged
Blood Cell Count
complete blood count
Female
haematology
Hematologic Tests - instrumentation
Hematologic Tests - methods
Hematologic Tests - standards
Humans
Laboratories - standards
Laboratory Proficiency Testing - standards
Male
Middle Aged
multicentre study
Original Papers
Quality Control
reference intervals
Reference Values
Regression Analysis
Reproducibility of Results
Turkey
Young Adult
haematology
reference intervals
Turkey
multicentre study
complete blood count
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Summary:A nationwide multicentre study was conducted to establish well-defined reference intervals (RIs) of haematological parameters for the Turkish population in consideration of sources of variation in reference values (RVs). K2-EDTA whole blood samples (total of 3363) were collected from 12 laboratories. Sera were also collected for measurements of iron, UIBC, TIBC, and ferritin for use in the latent abnormal values exclusion (LAVE) method. The blood samples were analysed within 2 hours in each laboratory using Cell Dyn and Ruby (Abbott), LH780 (Beckman Coulter), or XT-2000i (Sysmex). A panel of freshly prepared blood from 40 healthy volunteers was measured in common to assess any analyser-dependent bias in the measurements. The SD ratio (SDR) based on ANOVA was used to judge the need for partitioning RVs. RIs were computed by the parametric method with/without applying the LAVE method. Analyser-dependent bias was found for basophils (Bas), MCHC, RDW and MPV from the panel test results and thus those RIs were derived for each manufacturer. RIs were determined from all volunteers' results for WBC, neutrophils, lymphocytes, monocytes, eosinophils, MCV, MCH and platelets. Gender-specific RIs were required for RBC, haemoglobin, haematocrit, iron, UIBC and ferritin. Region-specific RIs were required for RBC, haemoglobin, haematocrit, UIBC, and TIBC. With the novel use of a freshly prepared blood panel, manufacturer-specific RIs' were derived for Bas, Bas%, MCHC, RDW and MPV. Regional differences in RIs were observed among the 7 regions of Turkey, which may be attributed to nutritional or environmental factors, including altitude.
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content type line 23
183387
ISSN:1330-0962
1846-7482
DOI:10.11613/BM.2017.038